Nonlinear microscopy: New techniques and applications

Current Opinion in Neurobiology

Volumn 14, Issue 5, 2004, Pages 610-616

  Mertz, Jerome ^a  

^a Boston University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MARKER;

BIOSENSOR; BRAIN NERVE CELL; CELL FUNCTION; CELL STRUCTURE; CONTRAST ENHANCEMENT; FLUORESCENCE MICROSCOPE; FLUORESCENCE MICROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER; IMAGING; MICROSCOPE; MICROSCOPY; NEUROBIOLOGY; NONLINEAR SYSTEM; OPTICS; PHOTOCHEMISTRY; PHOTON; PRIORITY JOURNAL; PROCESS DEVELOPMENT; REVIEW; SPECTRUM; TECHNIQUE; THREE DIMENSIONAL IMAGING;

ANIMALS; BIOLOGICAL MARKERS; FLUORESCENT DYES; HUMANS; MICROSCOPY; MICROSCOPY, FLUORESCENCE; NERVOUS SYSTEM; OPTICS; PHOTIC STIMULATION; STAINING AND LABELING;

EID: 5344259630     PISSN: 09594388     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.conb.2004.08.013     Document Type: Review

References (79)

1. W.R. Zipfel, R.M. Williams, and W.W. Webb Nonlinear magic: multiphoton microscopy in the biosciences Nat Biotechnol 21 2003 1369 1377
2. F. Bestvater, E. Spiess, G. Stobrawa, M. Hacker, T. Feurer, T. Porwol, U. Berchner-Pfannschmidt, C. Wotzlaw, and H. Acker Two-photon fluorescence absorption and emission spectra of dyes relevant for cell imaging J Microsc 208 2002 108 115
3. D.L. Wokosin, C.M. Loughrey, and G.L. Smith Characterization of a range of Fura dyes with two-photon excitation Biophys J 86 2004 1726 1738
4. N. Marandi, A. Konnerth, and O. Garaschuk Two-photon chloride imaging in neurons of brain slices Pflugers Arch 445 2002 357 365
5. M. Taki, J.L. Wolford, and T.V. O'Halloran Emission ratiometric imaging of intracellular zinc: design of benzoxazole fluorescent sensor and its application in two-photon microscopy J Am Chem Soc 126 2004 712 713
6. 2+ dynamics J Physiol 551.1 2003 67 78
7. + channels control action potential backpropagation J Physiol 551.1 2003 49 65
8. V. Egger, K. Svoboda, and Z.F. Mainen Mechanisms of lateral inhibition in the olfactory bulb: efficiency and modulation of spike-evoked calcium influx into granule cells J Neurosci 23 2003 7551 7558
9. 2+ signals evoked by coincident EPSPs and backpropagating action potentials in spiny stellate cells of layer 4 in the juvenile rate somatosensory barrel cortex J Neurosci 24 2004 1689 1699
10. 2+ influx dynamics into dendritic tufts of layer 2/3 neocortical pyramidal neurons in-vitro and in-vivo J Neurosci 23 2003 8558 8567
11. F. Debarbieux, E. Audinat, and S. Charpak Action potential propagation in dendrites of rat mitral cells in vivo J Neurosci 23 2003 5553 5560
12. J.H. Goldberg, G. Tamas, D. Aronov, and R. Yuste Calcium microdomains in aspiny dendrites Neuron 40 2003 807 821
13. R. Yasuda, B.L. Sabatini, and K. Svoboda Plasticity of calcium channels in dendritic spines Nat Neurosci 6 2003 948 955
14. E.A. Nimchinsky, R. Yasuda, T.G. Oertner, and K. Svoboda The number of glutamate receptors opened by synaptic stimulation in single hippocampal spines J Neurosci 24 2004 2054 2064
15. R. Cossart, D. Aronov, and R. Yuste Attractor dynamics of network UP states in the neocortex Nature 423 2003 283 288 The authors investigate the calcium dynamics of up to 1,400 cells in mice brain slices bathed in a Fura2-AM solution, revealing the occurrence of spontaneous synchronized high-frequency firing in coordinated networks of neurons. Cells involved in synchronized activity exhibit bimodal membrane potentials that simultaneously shift to suprathreshold UP (versus DOWN) states.
16. C. Stosiek, O. Garaschuk, K. Holthoff, and A. Konnerth In vivo two-photon calcium imaging of neuronal networks Proc Natl Acad Sci USA 100 2003 7319 7324 The authors use a patch pipette and a pressure ejection-based protocol to deliver locally a variety of AM esters of calcium sensitive dyes into mouse brain tissue in vivo. The ejection volume was ∼400 femtoliters, allowing the possibility of TPEF intracellular calcium response imaging of targeted neuronal networks with single cell resolution. The technique is suitable for mice of all ages.
17. 2+ imaging Pflugers Arch 446 2003 766 773
18. B.J. Backsai, G.A. Hickey, J. Skoch, S.T. Kajdasz, Y. Wang, G.-F. Huang, C.A. Mathis, W.E. Klunk, and B.T. Hyman Four-dimensional multiphoton imaging of brain entry, amyloid binding, and clearance of an amyloid-β ligand in transgenic mice Proc Natl Acad Sci USA 100 2003 12462 12467 TPEF microscopy reveals that Pittsburg compound B, which traverses the blood-brain barrier, is an excellent biomarker for the detection of amyloid-β plaques. This molecule can be readily tagged with a positron emission tomography contrast agent, paving the way for the early detection of Alzheimer's disease.
19. D. Kleinfeld, P. Mitra, F. Helmchen, and W. Denk Fluctuations and stimulus induced changes in blood flow observed in individual capillaries in layers 2 through 4 of rat neocortex Proc Natl Acad Sci USA 95 1998 15741 15746
20. E. Chaigneau, M. Oheim, E. Audinat, and S. Charpak Two-photon imaging of capillary blood flow in olfactory bulb glomeruli Proc Natl Acad Sci USA 100 2003 13081 13086
21. D.R. Larson, W.R. Zipfel, R.M. Williams, S.W. Clark, M.P. Bruchez, F.W. Wise, and W.W. Webb Water-soluble quantum dots for multiphoton fluorescence imaging in vivo Science 300 2003 1434 1436 The authors show that water-soluble QDs are more efficient at producing TPEF by at least two orders of magnitude compared to the best standard water-soluble fluorescent dyes. These enable exceptionally deep imaging of capillaries in the skin tissue of living mice, and allow the possibility of multicolor emission imaging.
22. M. Dahan, S. Levi, C. Luccardi, P. Rostaing, B. Riveau, and A. Triller Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking Science 302 2003 442 445
23. G.T. Hanson, T.B. McAnaney, E.S. Park, E.P. Rendell, D.K. Yarbrough, S. Chu, L. Xi, S.G. Boxer, M.H. Montrose, and S.J. Remington Green fluorescent protein variant as ratiometric dual emission pH sensors. 1. Structural characterization and preliminary application Biochemistry 41 2002 15477 15488
24. W.R. Zipfel, R.M. Williams, R. Christie, A.Y. Nikitin, B.T. Hyman, and W.W. Webb Live tissue intrinsic emission microscopy using multi-photon excited native fluorescence and second harmonic generation Proc Natl Acad Sci USA 100 2003 7075 7080
25. J. Balaji, C.S. Reddy, S.K. Kaushalya, and S. Maiti Microfluometric detection of catecholamines with multiphoton-excited fluorescence Appl Opt 43 2004 2412 2417
26. M.L. Gostkowski, R. Allen, M.L. Plenert, E. Okerberg, M.J. Gordon, and J.B. Shear Multiphoton-excited serotonin photochemistry Biophys J 86 2004 3223 3229
27. J. Rathenberg, T. Nevian, and V. Witzemann High-efficiency transfection of individual neurons using modified electrophysiology techniques J Neurosci Methods 126 2003 91 98
28. U.K. Tirlapur, and K. Konig Targeted transfection by femtosecond laser Nature 418 2002 290 291
29. A. Tashiro, A. Dunaevsky, R. Blazeski, C.A. Mason, and R. Yuste Bidirectional regulation of hippocampal mossy fiber filopodial motility by kainate receptors: a two-step model of synaptogenesis Neuron 38 2003 773 784
30. T.W. Margrie, A.H. Meyer, A. Caputi, H. Monyer, M.T. Hasan, A.T. Schaefer, W. Denk, and M. Brecht Targeted whole-cell recordings in the mammalian brain in-vivo Neuron 39 2003 911 918 The authors use TPEF from GFP labeled cortical interneurons to guide a pipette and enable targeted whole-cell patch recordings in vivo. In effect, GFP contrast is used here in vivo in a similar way as differential interference contrast (DIC) is used in standard electrophysiology experiments with slices. GFP labeling provides the added benefit that it facilitates identification of specific cellular populations.
31. J.T. Trachtenberg, B.E. Chen, G.E. Knott, G. Feng, J.R. Sanes, E. Welker, and K. Svoboda Long-term in vivo imaging of experience-dependent synaptic plasticity in adult cortex Nature 420 2002 788 793
32. A. Mizrahi, and L.C. Katz Dendritic stability in the adult olfactory bulb Nat Neurosci 6 2003 1201 1207
33. A. Majewska, and M. Sur Motility of dendritic spines in visual cortex in vivo: changes during the critical period and effects of visual deprivation Proc Natl Acad Sci USA 100 2003 16024 16029
34. J. Grutzendler, N. Kasthuri, and W.-B. Gan Long-term dendritic spine stability in the adult cortex Nature 420 2002 812 816
35. T. Das, B. Payer, M. Cayouette, and W.A. Harris In vivo time-lapse imaging of cell divisions during neurogenesis in the developing zebrafish retina Neuron 37 2003 597 609
36. 2+ imaging.
37. A.G. Harpur, F.S. Wouters, and Bastiaens PIH Imaging FRET between spectrally similar GFP molecules in single cells Nat Biotechnol 19 2001 167 169
38. Y. Chen, J.D. Mills, and A. Periasamy Protein localization in living cells and tissues using FRET and FLIM Differentiation 71 2003 528 541
39. B.J. Backsai, J. Skoch, G.A. Hickey, R. Allen, and B.T. Hyman Fluorescence resonance energy transfer determinations using multiphoton fluorescence lifetime imaging microscopy to characterize amyloid-beta plaques J Biomed Opt 8 2003 368 375
40. R.V. Krishnan, A. Masuda, V.E. Centonze, and B. Herman Quantitative imaging of protein-protein interactions by multiphoton fluorescence lifetime imaging microscopy using a streak camera J Biomed Opt 8 2003 362 367
41. W. Denk Two-photon scanning photochemical microscopy: mapping ligand-gated ion channel distributions Proc Natl Acad Sci USA 91 1994 6629 6633
42. 2+ movements J Biomed Opt 8 2003 418 427
43. 2+ signaling in mouse cortical neurons studied by two-photon imaging and photoreleased inositol triphosphate J Neurosci 23 2003 758 765
44. E.M. Callaway, and R. Yuste Stimulating neurons with light Curr Opin Neurobiol 12 2002 587 592
45. H. Hirase, V. Nikolenko, J.H. Goldberg, and R. Yuste Multiphoton stimulation of neurons J Neurobiol 51 2002 237 247
46. T. Pons, L. Moreaux, and J. Mertz Photoinduced flip-flop of amphiphilic molecules in lipid bilayer membranes Phys Rev Lett 89 2002 288104
47. K. Konig, I. Riemann, and W. Fritzsche Nanodissection of human chromosomes with near-infrared femtosecond laser pulses Opt Lett 26 2001 819 821
48. Shen N, Schaffer CB, Datta D, Mazur E: Photodisruption in biological tissues and single cells using femtosecond laser pulses. In Conference on Lasers and ElectroOptics (CLEO); Baltimore, MD: Optical Society of America: 2001:403-404.
49. E. Beaurepaire, M. Oheim, and J. Mertz Ultra-deep two-photon fluorescence excitation in turbid media Opt Commun 188 2001 25 29
50. 3 regenerative amplifier Opt Lett 28 2003 1022 1024 The authors further extend the principle of using pulsed excitation to produce TPEF. By amplifying laser pulse power at the expense of pulse repetition rate (keeping average power unchanged), they demonstrate a significant gain in depth penetration in brain tissue (see Figure 2). This strategy is appropriate when multiphoton induced photodamage is negligible and the only limit to depth penetration is insufficient laser power.
51. W. Denk, K.R. Delaney, A. Gelperin, D. Kleinfeld, B.W. Strowbridge, D.W. Tank, and R. Yuste Anatomical and functional imaging of neurons using 2-photon laser-scanning microscopy J Neurosci Methods 54 1994 151 162
52. E. Beaurepaire, and J. Mertz Epi-fluorescence collection in two-photon microscopy Appl Opt 41 2002 5376 5382
53. Neil MAA, R. Juskaitis, M.J. Booth, T. Wilson, T. Tanaka, and S. Kawata Adaptive aberration correction in a two-photon microscope J Microsc 200 2000 105 108
54. M.J. Booth, M.A.A. Neil, and T. Wilson New modal wave-front sensor: application to adaptive confocal fluorescence microscopy and two-photon excitation fluorescence microscopy J Opt Soc Am A Opt Image Sci Vis 19 2002 2112 2120
55. L. Sherman, J.Y. Ye, O. Albert, and T.B. Norris Adaptive correction of depth-induced aberrations in multiphoton scanning microscopy using a deformable mirror J Microsc 206 2002 65 71
56. P.N. Marsh, D. Burns, and J.M. Girkin Practical implementation of adaptive optics in multiphoton microscopy Opt Express 11 2003 1123 1130
57. A. Mizrahi, J.C. Crowley, E. Shtoyerman, and L.C. Katz High-resolution in-vivo imaging of hippocampal dendrites and spines J Neurosci 24 2004 3147 3151
58. P.S. Tsai, B. Friedman, A.I. Ifarraguerri, B.D. Thompson, V. Lev-Ram, C.B. Schaffer, Q. Xiong, R.Y. Tsien, J.A. Squier, and D. Kleinfeld All-optical histology using ultrashort laser pulses Neuron 39 2003 27 41
59. J.C. Jung, and M.J. Schnitzer Multiphoton endoscopy Opt Lett 28 2003 902 904
60. M.J. Levene, D.A. Dombeck, K.A. Kasischke, R.P. Molloy, and W.W. Webb In vivo multiphoton microscopy of deep brain tissue J Neurophysiol 91 2004 1908 1912 The authors demonstrate a panoply of TPEF neuroimaging possibilities with a needle-like GRIN lens, including in vivo microangiography with QDs, deep cortical imaging of yellow fluorescent protein marked neurons and axon bundles, and imaging of the hippocampal neuropil down to a depth of ∼1.5 mm. The insertion of the GRIN lens into the brain apparently leads to little tissue trauma.
61. F. Helmchen, D.W. Tank, and W. Denk Enhanced two-photon excitation through optical fiber by single-mode propagation in a large core Appl Opt 41 2002 2930 2934
62. D.G. Ouzounov, K.D. Moll, M.A. Foster, W.R. Zipfel, W.W. Webb, and A.L. Gaeta Delivery of nanojoule femtosecond pulses through large-core microstructured fibers Opt Lett 27 2002 1513 1515
63. V. Andresen, A. Egner, and S.W. Hell Time-multiplexed multifocal multiphoton microscope Opt Lett 26 2001 75 77
64. M. Kobayashi, K. Fujita, T. Kaneko, T. Takamatsu, O. Nakamura, and S. Kawata Second-harmonic-generation microscope with a microlens array scanner Opt Lett 27 2002 1324 1326
65. L. Sacconi, E. Fromer, R. Antolini, M.R. Taghizadeh, A. Choudhury, and F.S. Pavone Multiphoton multifocal microscopy exploiting a diffractive optical element Opt Lett 28 2003 1918 1920
66. J.D. Lechleiter, D.-T. Lin, and I. Sieneart Multi-photon laser scanning microscopy using an acoustic optical deflector Biophys J 83 2002 2292 2299
67. V. Iyer, B.E. Losavio, and P. Saggau Compensation of spatial and temporal dispersion for acousto-optic multiphoton laser-scanning microscopy J Biomed Opt 8 2003 460 471
68. R.D. Roorda, T.M. Hohl, R. Toledo-Crow, and G. Miesenbock Video-rate nonlinear microscopy of neuronal membrane dynamics with genetically encoded probes J Neurophysiol 69 2004 609 621
69. J. Mertz, and L. Moreaux Second harmonic generation by focused excitation of inhomogeneously distributed scatterers Opt Commun 196 2001 325 330
70. S.-W. Chu, T.-M. Liu, C.-K. Sun, C.-Y. Lin, and H.-J. Tsai Real-time second-harmonic-generation microscopy based on a 2-GHz repetition rate Ti:sapphire laser Opt Express 11 2003 933 938
71. A.C. Millard, L. Jin, M.-D. Wei, J.P. Wuskell, A. Lewis, and L.M. Loew Sensitivity of second harmonic generation from styryl dyes to transmembrane potential Biophys J 86 2004 1169 1176
72. L. Moreaux, T. Pons, V. Dambrin, M. Blanchard-Desce, and J. Mertz Electro-optic response of second harmonic generation membrane potential sensors Opt Lett 28 2003 625 627
73. T. Pons, L. Moreaux, O. Mongin, M. Blanchard-Desce, and J. Mertz Mechanisms of membrane potential sensing with second-harmonic generation microscopy J Biomed Opt 8 2003 428 431
74. D.A. Dombeck, M. Blanchard-Desce, and W.W. Webb Optical recording of action potentials with second-harmonic generation microscopy J Neurosci 24 2004 999 1003
75. L. Moreaux, O. Sandre, S. Charpak, M. Blanchard-Desce, and J. Mertz Coherent scattering in multi-harmonic light microscopy Biophys J 80 2001 1568 1574
76. P.J. Campagnola, and L.M. Loew Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms Nat Biotechnol 21 2003 1356 1360
77. D.A. Dombeck, K.A. Kasischke, H.D. Vishwasrao, M. Ingelsson, B.T. Hyman, and W.W. Webb Uniform polarity microtubule assemblies imaged in native brain tissue by second harmonic generation microscopy Proc Natl Acad Sci USA 100 2003 7081 7086 SHG produces contrast in native unlabeled brain tissue, which the authors demonstrate to arise from microtubule assemblies in neuronal processes. Because SHG requires an asymmetric source, it is only generated when the individual tubulin strands in these assemblies exhibit the same polarity, allowing the identification of both structure and function of developing neuronal processes (see Figure 3).
78. E.O. Potma, W.P. de Boeij, van Haastert PJM, and D.A. Wiersma Real-time visualization of intracellular hydrodynamics in single living cells Proc Natl Acad Sci USA 98 2001 1577 1582
79. J.-X. Cheng, S. Pautot, D.A. Weitz, and X. Sunney Xie Ordering of water molecules between phospholipid bilayers visualized by coherent anti-Stokes Raman scattering microscopy Proc Natl Acad Sci USA 100 2003 9826 9830