High-efficiency transfection of individual neurons using modified electrophysiology techniques

Journal of Neuroscience Methods

Volumn 126, Issue 1, 2003, Pages 91-98

  Rathenberg, Jan ^a   Nevian, Thomas ^a   Witzemann, Veit ^a  

^a MAX PLANCK INSTITUTE FOR MEDICAL RESEARCH   (Germany)

Author keywords

Electrophysiology; Green fluorescent protein; Neuron; Organotypic brain slice culture; Single cell electroporation; Transfection

Indexed keywords

GREEN FLUORESCENT PROTEIN; OLIGONUCLEOTIDE; PLASMID DNA;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN CORTEX; BRAIN SLICE; CELL MEMBRANE TRANSPORT; ELECTRON MICROSCOPY; ELECTROPHYSIOLOGY; ELECTROPORATION; FLUORESCENCE; GENETIC TRANSFECTION; HIPPOCAMPUS; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; RAT;

EID: 0038208235     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(03)00069-4     Document Type: Article

References (15)

1. Carroll P.T. Membrane-bound choline-O-acetyltransferase in rat hippocampal tissue is associated with synaptic vesicles. Brain Res. 633:1994;112-118.
2. Denk W., Strickler J.H., Webb W.W. Two-photon laser scanning fluorescence microscopy. Science. 248:1990;73-76.
3. Golzio M., Teissie J., Rols M.P. Direct visualization at the single-cell level of electrically mediated gene delivery. Proc. Natl. Acad. Sci. USA. 99:2002;1292-1297.
4. Haas K., Sin W.C., Javaherian A., Li Z., Cline H.T. Single-cell electroporation for gene transfer in vivo. Neuron. 29:2001;583-591.
5. Kaether C., Skehel P., Dotti C.G. Axonal membrane proteins are transported in distinct carriers: a two-color video microscopy study in cultured hippocampal neurons. Mol. Biol. Cell. 11:2000;1213-1224.
6. Neumann E., Kakorin S., Toensing K. Fundamentals of electroporative delivery of drugs and genes. Bioelectrochem. Bioenerg. 48:1999;3-16.
7. Rae J.L., Levis R.A. Single-cell electroporation. Pflugers Arch. 443:2002;664-670.
8. Rathenberg J., Gartner A., Koenen M., Witzemann V. Modification of choline acetyltransferase by integration of green fluorescent protein does not affect enzyme activity and subcellular distribution. Cell Tissue Res. 308:2002;1-6.
9. Schiefer J., Kampe K., Dodt H.U., Zieglgansberger W., Kreutzberg G.W. Microglial motility in the rat facial nucleus following peripheral axotomy. J. Neurocytol. 28:1999;439-453.
10. Shi S., Hayashi Y., Esteban J.A., Malinow R. Subunit-specific rules governing AMPA receptor trafficking to synapses in hippocampal pyramidal neurons. Cell. 105:2001;331-343.
11. Stoppini L., Buchs P.A., Muller D. A simple method for organotypic cultures of nervous tissue. J. Neurosci. Methods. 37:1991;173-182.
12. Stuart G.J., Dodt H.U., Sakmann B. Patch-clamp recordings from the soma and dendrites of neurons in brain slices using infrared video microscopy. Pflugers Arch. 423:1993;511-518.
13. Teruel M.N., Meyer T. Electroporation-induced formation of individual calcium entry sites in the cell body and processes of adherent cells. Biophys. J. 73:1997;1785-1796.
14. Teruel M.N., Blanpied T.A., Shen K., Augustine G.J., Meyer T. A versatile microporation technique for the transfection of cultured CNS neurons. J. Neurosci. Methods. 93:1999;37-48.
15. Van den Pol A.N., Ghosh P.K. Selective neuronal expression of green fluorescent protein with cytomegalovirus promoter reveals entire neuronal arbour in transgenic mice. J. Neurosci. 18:1998;10640-10651.