Imaging FRET between spectrally similar GFP molecules in single cells

Nature Biotechnology

Volumn 19, Issue 2, 2001, Pages 167-169

  Harpur, Ailsa G ^a   Wouters, Fred S ^a   Bastiaens, Philippe I H ^a,b  

^a IMPERIAL CANCER RESEARCH FUND   (United Kingdom)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM ION; CASPASE; GREEN FLUORESCENT PROTEIN; PROTEINASE;

APOPTOSIS; ARTICLE; CALCIUM TRANSPORT; ENERGY TRANSFER; FLUORESCENCE; IMAGE ANALYSIS; MICROSCOPIC ANATOMY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN VARIANT; REPRODUCIBILITY;

ANIMALS; APOPTOSIS; BACTERIAL PROTEINS; CASPASE 3; CASPASES; CELL LINE; ENERGY TRANSFER; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MAMMALS; MICROSCOPY, FLUORESCENCE; PROTEIN CONFORMATION; RATS; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, FLUORESCENCE; TRANSFECTION; VARIATION (GENETICS);

EID: 0035122528     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/84443     Document Type: Article

References (20)

1. Zwischenmolekulare energiewanderung und fluoreszenz
2. Using GFP in FRET-based applications
3. 2+ based on green fluorescent proteins and calmodulin
4. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluoresence resonance energy transfer
5. Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein
6. Detection of programmed cell death using fluorescence energy transfer
7. Novel mutant green fluorescent protein protease substrates reveal the activation of specific caspases during apoptosis
8. A fluorescent indicator for visualizing cAMP-induced phosphorylation in vivo
9. Three dimensional image restoration in fluorescence lifetime imaging
10. Time resolved imaging fluorescence microscopy
11. Fluorescence lifetime imaging microscopy (FLIM) - Spatial resolution of microstructures on the nanosecond time-scale
12. Lifetime-selective fluorescence imaging using an rf phase-sensitive camera
13. Degradation of retinoblastoma protein in tumor necrosis factor- and CD95-induced cell death
14. Proteolysis of fodrin (non-erythroid spectrin) during apoptosis
15. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE
16. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis
17. Specific cleavage of the 70-kDa protein component of the U1 small nuclear ribonucleoprotein is a characteristic biochemical feature of apoptotic cell death
18. Simultaneous detection of multiple green fluorescent proteins in live cells
19. A modified oestrogen receptor ligand-binding domain as an improved switch for the regulation of heterologous proteins
20. Requirement for the CD95 receptor-ligand pathway in c-Myc-induced apoptosis