Two-photon fluorescence absorption and emission spectra of dyes relevant for cell imaging

Journal of Microscopy

Volumn 208, Issue 2, 2002, Pages 108-115

  Bestvater, F ^a   Spiess, E ^a   Stobrawa, G ^b   Hacker, M ^b   Feurer, T ^b   Porwol, T ^c   Berchner Pfannschmidt, U ^c   Wotzlaw, C ^c   Acker, H ^c  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^b FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^c MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

Author keywords

3D reconstruction; Fluorochromes; Live cell imaging; MPM; Non linear microscopy; Spectra; TPE; Two photon excitation

Indexed keywords

ABSORPTION SPECTROSCOPY; CELL PROLIFERATION; EMISSION SPECTROSCOPY; FLUORESCENCE MICROSCOPY; FLUOROPHORES; IMAGE RECONSTRUCTION; LASER EXCITATION; OPTICAL PARAMETRIC AMPLIFIERS; PARAMETRIC AMPLIFIERS; SAPPHIRE; TWO PHOTON PROCESSES;

3D RECONSTRUCTION; ABSORPTION AND EMISSION SPECTRA; CELL IMAGING; FLUOROCHROMES; LIVE-CELL IMAGING; MPM; NONLINEAR MICROSCOPY; SPECTRA'S; TWO PHOTON FLUORESCENCE; TWO-PHOTON EXCITATIONS;

PHOTONS;

4',6 DIAMIDINO 2 PHENYLINDOLE; ALEXA FLUOR 488; ALEXA FLUOR 546; ALEXA FLUOR 594; ANTIBODY; BODIPY FLA; BODIPY TROAD; CY2 DYE; CY3 DYE; DNA; DYE; FLUORESCEIN ISOTHIOCYANATE; FLUORESCENT DYE; FLUOROCHROME; HOE 33342; PROPIDIUM IODIDE; RHODAMINE; UNCLASSIFIED DRUG;

AMPLIFIER; ANALYZER; ARTICLE; CELL; CELL ORGANELLE; CELL STRUCTURE; CONTROLLED STUDY; FLAME PHOTOMETRY; FLUORESCENCE MICROSCOPY; FLUORESCENCE SPECTROSCOPY; HUMAN; HUMAN CELL; LASER; LASER MICROSCOPY; PRIORITY JOURNAL; THEORY; THREE DIMENSIONAL IMAGING;

ALEXA;

EID: 0036434346     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.2002.01074.x     Document Type: Article

References (23)

1. Albota, M.A., Xu, C. & Webb, W.W. (1998) Two-photon fluorescence excitation cross sections of biomeolecular probes from 690 to 960 nm. Appl. Opt.37, 7352-7356.
2. Bestvater, F., Knoch, T.A., Langowski, J. & Spiess, E. (2002) Construct conversions caused by simultaneous cotransfection: 'GFP-walking'. Biotechniques, 32, 830-836.
3. Birge, R.R. (1986) Two-photon spectroscopy of protein bound chromophores. Acc. Chem. Res. 19, 138-146.
4. Brown, E.B., Campell, R.B., Tsuzuki, Y., Xu, L., Carmeliet, P., Fukumura, D. & Jain, R. (2001)In vivo measurement of gene expression, angiogenesis and physiological function in tumours using multiphoton laser scanning microscopy. Nature Med. 7, 864-868.
5. Centonze, V.E. & White, J.G. (1998) Multiphoton excitation provides optical sections from deeper within scattering specimens than confocal imaging. Biophys. J. 75, 2015-2024.
6. Denk, W., Piston, D.W. & Webb, W.W. (1995) Two-photon molecular excitation in laser scanning microscopy. Handbook of Biological Confocal Microscopy (ed. by J. B. Pawley), pp. 445-458. Plenum Press, New York.
7. Denk, W., Strickler, J.H. & Webb, W. (1990) Two-photon laser scanning fluorescence microscopy. Science, 248, 73-76.
8. Denk, W. & Svoboda, K. (1997) Photon upmanship. Why multiphoton imaging is more than a gimmick. Neuron, 18, 351-357.
9. Fisher, W.G., Wachter, E.A., Lytle, F.E., Armas, M. & Seaton, C. (1998) Source-corrected two-photon excited fluorescence measurements between 700 and 880 nm. Appl. Spect. 52, 536-545.
10. Gratton, E., Barry, N.P., Beretta, S. & Celli, A. (2001) Multiphoton fluorescence microscopy. Methods, 25, 103-110.
11. Hell, S.W., Booth, M., Wilms, S., Schnetter, C.M., Kirsch, A.K., Arndt-Jovin, D.J. & Jovin, T. (1998) Two-photon near and far-field fluorescence microscopy with continuous-wave excitation. Opt. Lett. 23, 1238-1240.
12. König, K. (2000) Multiphoton microscopy in life sciences. J. Microsc. 200, 82-104.
13. Malak, H., Castellano, F.N., Gryczinski, I. & Lakowicz, J.R. (1997) Two-photon excitation of ethidium bromide labeled DNA. Biophys. Chem. 67, 35-41.
14. Patterson, G.H. & Piston, D.W. (2000) Photobleaching in two-photon excitation microscopy. Biophys. J. 78, 2159-2162.
15. Periasamy, A., Skoglund, P., Noakes, C. & Keller, R. (1999) An evaluation of two-photon excitation versus confocal and digital deconvolution fluorescence microscopy imaging inXenopus morphogenesis. Microsc. Res. Techn, 47, 172-181.
16. Piston, D.W. (1999) Imaging living cells and tissues by two-photon excitation microscopy. Trends Cell Biol. 9, 66-69.
17. Sheppard, C.J.R. (1977) The use of lenses with annular aperture in scanning optical microscopy. Optik, 48, 329-334.
18. Smith, W.L. (1986) Two-photon absorption in condensed media.Hand-book of Laser Science and Technology (ed. by J. Weber), pp. 229-258. CRC, Boca Raton, Florida
19. Straub, M., Lodemann, P., Holroyd, P., Jahn, R. & Hell, S.W. (2000) Live cell imaging by multiphoton microscopy. Eur. J. Cell Biol. 79, 726-734.
20. Williams, R.M., Piston, D.W. & Webb, W.W. (1994) Two-photon molecular excitation provides intrinsic 3-dimensional resolution for laser-based microscopy and microphotochemistry. FASEB J. 8, 804-813.
21. Williams, R.M., Zipfel, W.R. & Watt, W.W. (2001) Multiphoton microscopy in biological research. Curr. Opin. Chem. Biol. 5, 603-608.
22. Xu, C. & Webb, W. (1996) Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm. J. Opt. Soc. Am. B. 13, 481-491.
23. Xu, C., Zipfel, W., Shear, J.B., Williams, R.M. & Webb, W.W. (1996) Multiphoton fluorescence excitation: New spectral windows for biological non-linear microscopy. Proc. Natl. Acad. Sci. USA, 93, 10763-10768.