Optical Recording of Action Potentials with Second-Harmonic Generation Microscopy

Journal of Neuroscience

Volumn 24, Issue 4, 2004, Pages 999-1003

  Dombeck, Daniel A ^a   Blanchard Desce, Mireille ^b   Webb, Watt W ^a  

^a School of Operations Research and Industrial Engineering   (United States)

^b UNIVERSITÉ DE RENNES 1   (France)

Author keywords

Action potential; Imaging; Membrane potential; Microscopy; Nonlinear; Optical; Second harmonic generation; Voltage sensitive dye

Indexed keywords

4 [4 (DIHEXYLAMINO)PHENYL]ETHYNYL 1 (SULFOBUTYL)PYRIDINIUM; PYRIDINIUM DERIVATIVE; UNCLASSIFIED DRUG;

ACTION POTENTIAL; ANIMAL CELL; APLYSIA; ARTICLE; BLEACHING; CELL STRUCTURE; CULTURE MEDIUM; ELECTRIC ACTIVITY; ELECTROPHYSIOLOGY; IMAGE ANALYSIS; IMAGE PROCESSING; IMAGE QUALITY; IMAGING SYSTEM; LASER; MICROSCOPY; NERVE CELL; NERVE CELL CULTURE; NERVE CELL MEMBRANE; NERVE CELL MEMBRANE POTENTIAL; NEURITE; NEUROSCIENCE; NONHUMAN; OPTICAL RESOLUTION; OPTICS; PHOTOSTIMULATION; PRIORITY JOURNAL; SECOND HARMONIC GENERATION MICROSCOPY; SIGNAL NOISE RATIO;

ACTION POTENTIALS; ANIMALS; APLYSIA; CELLS, CULTURED; DERMATITIS, PHOTOTOXIC; ELECTRIC STIMULATION; FLUORESCENT DYES; GANGLIA, INVERTEBRATE; MICROSCOPY; NEURONS; OPTICS; PATCH-CLAMP TECHNIQUES; REACTION TIME; SENSITIVITY AND SPECIFICITY;

EID: 0742287636     PISSN: 02706474     EISSN: None     Source Type: Journal    
DOI: 10.1523/JNEUROSCI.4840-03.2004     Document Type: Article

References (39)

1. Antic S, Major G, Zecevic D (1999) Fast optical recordings of membrane potential changes from dendrites of pyramidal neurons. J Neurophysiol 82:1615-1621.
2. Bedi SS, Salim A, Chen S, Glanzman DL (1998) Long-term effects of axotomy on excitability and growth of isolated Aplysia sensory neurons in cell culture: potential role of cAMP. J Neurophysiol 79:1371-1383.
3. Ben-Oren I, Peleg G, Lewis A, Minke B, Loew L (1996) Infrared nonlinear optical measurements of membrane potential in photoreceptor cells. Biophys J 71:1616-1620.
4. Bouevitch O, Lewis A, Pinevsky I, Wuskell JP, Loew LM (1993) Probing membrane potential with nonlinear optics. Biophys J 65:672-679.
5. Bullen A, Saggau P (1999) High-speed, random-access fluorescence microscopy. 11. Fast quantitative measurements with voltage-sensitive dyes. Biophys J 76:2272-2287.
6. Bullen A, Patel SS, Saggau P (1997) High-speed, random-access fluorescence microscopy. I. High-resolution optical recording with voltage-sensitive dyes and ion indicators. Biophys J 73:477-491.
7. Campagnola PJ, Wei MD, Lewis A, Loew LM (1999) High-resolution non-linear optical imaging of live cells by second harmonic generation. Biophys J 77:3341-3349.
8. Cohen LB, Keynes RD, Hille B (1968) Light scattering and birefringence changes during nerve activity. Nature 218:438-441.
9. Delaney KR, Gelperin A, Fee MS, Flores JA, Gervais R, Tank DW, Kleinfeld D (1994) Waves and stimulus-modulated dynamics in an oscillating olfactory network. Proc Natl Acad Sci USA 91:669-673.
10. Denk W, Svoboda K (1997) Photon upmanship: why multiphoton imaging is more than a gimmick. Neuron 18:351-357.
11. Denk W, Strickler JH, Webb WW (1990) Two-photon laser scanning fluorescence microscopy. Science 248:73-76.
12. Dombeck DA, Kasischke KA, Vishwasrao HD, Ingelsson M, Hyman BT, Webb WW (2003) Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy. Proc Natl Acad Sci USA 100:7081-7086.
13. Fromherz P, Muller CO (1994) Cable properties of a straight neurite of a leech neuron probed by a voltage-sensitive dye. Proc Natl Acad Sci USA 91:4604-4608.
14. Gan WB, Grutzendler J, Wong WT, Wong RO, Lichtman JW (2000) Multicolor "DiOlistic" labeling of the nervous system using lipophilic dye combinations. Neuron 27:219-225.
15. Gardner DR, Kerkut GA (1968) A comparison of the effects of sodium and lithium ions on action potentials from Helix aspersa neurones. Comp Biochem Physiol 25:33-48.
16. Goldberg D, Schacher S (1998) Culturing large neurons of Aplysia. In: Culturing nerve cells, Ed 2 (Banker G, Goslin K, eds), pp 213-236. Cambridge, MA: MIT.
17. Gonzalez JE, Tsien RY (1997) Improved indicators of cell membrane potential that use fluorescence resonance energy transfer. Chem Biol 4:269-277.
18. Grinvald A, Hildesheim R, Farber IC, Anglister L (1982) Improved fluorescent probes for the measurement of rapid changes in membrane potential. Biophys J 39:301-308.
19. Knopfel T, Tomita K, Shimazaki R, Sakai R (2003) Optical recordings of membrane potential using genetically targeted voltage-sensitive fluorescent proteins. Methods 30:42-48.
20. Kobayashi M, Fujita K, Kaneko T, Takamatsu T, Nakamura O, Kawata S (2002) Second-harmonic generation microscope with a microlens array scanner. Opt Lett 27:1324-1326.
21. Loew LM, Cohen LB, Dix J, Fluhler EN, Montana V, Salama G, Wu JY (1992) A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations. J Membr Biol 130:1-10.
22. Meyer E, Muller CO, Fromherz P (1997) Cable properties of dendrites in hippocampal neurons of the rat mapped by a voltage-sensitive dye. Eur J Neurosci 9:778-785.
23. Millard AC, Jin L, Lewis A, Loew LM (2003) Direct measurement of the voltage sensitivity of second-harmonic generation from a membrane dye in patch-clamped cells. Opt Lett 28:1221-1223.
24. Moreaux L, Sandre O, Mertz J (2000) Membrane imaging by second-harmonic generation microscopy. J Opt Soc Am B 17:1685-1694.
25. Moreaux L, Sandre O, Charpak S, Blanchard-Desce M, Mertz J (2001) Coherent scattering in multi-harmonic light microscopy. Biophys J 80:1568-1574.
26. Moreaux L, Pons T, Dambrin V, Blanchard-Desce M, Mertz J (2003) Electro-optic response of second-harmonic generation membrane potential sensors. Opt Lett 28:625-627.
27. Pons T, Moreaux L, Mongin O, Blanchard-Desce M, Mertz J (2003) Mechanisms of membrane potential sensing with second-harmonic generation microscopy. J Biomed Opt 8:428-431.
28. Rink TJ, Montecucco C, Hesketh TR, Tsien RY (1980) Lymphocyte membrane potential assessed with fluorescent probes. Biochim Biophys Acta 595:15-30.
29. Rohr S, Salzberg BM (1994) Multiple site optical recording of transmembrane voltage (MSORTV) in patterned growth heart cell cultures: assessing electrical behavior, with microsecond resolution, on a cellular and subcellular scale. Biophys J 67:1301-1315.
30. Rubakhin SS, Li L, Moroz TP, Sweedler JV (1999) Characterization of the Aplysia californica cerebral ganglion F cluster. J Neurophysiol 81:1251-1260.
31. So PTC (2003) Modern applications of 3-D optical microscopy in biology and medicine. Conference on Lasers and Electro-Optics-Quantum Electronics and Laser Science Conference, Baltimore, June.
32. Stepnoski RA, LaPorta A, Raccuia-Behling F, Blonder GE, Slusher RE, Kleinfeld D (1991) Noninvasive detection of changes in membrane potential in cultured neurons by light scattering. Proc Natl Acad Sci USA 88:9382-9386.
33. Stosiek C, Garaschuk O, Hohhoff K, Konnerth A (2003) In vivo two-photon calcium imaging of neuronal networks. Proc Natl Acad Sci USA 100:7319-7324.
34. Stuart GJ, Sakmann B (1994) Active propagation of somatic action potentials into neocortical pyramidal cell dendrites. Nature 367:69-72.
35. Szmacinski H, Gryczynski I, Lakowicz JR (1993) Calcium-dependent fluorescence lifetimes of Indo-1 for one- and two-photon excitation of fluorescence. Photochem Photobiol 58:341-345.
36. Tsien RY, Bacskai BJ (1995) Video-rate confocal microscopy. In: Handbook of biological con focal microscopy, Ed 2 (Pawley JB, ed), pp 459-477. New York: Plenum.
37. Yuste R, Denk W (1995) Dendritic spines as basic functional units of neuronal integration. Nature 375:682-684.
38. Zecevic D (1996) Multiple spike-initiation zones in single neurons revealed by voltage-sensitive dyes. Nature 381:322-325.
39. Zochowski M, Wachowiak M, Falk CX, Cohen LB, Lam YW, Antic S, Zecevic D (2000) Imaging membrane potential with voltage-sensitive dyes. Biol Bull 198:1-21.