Ribosome display for selection of active dihydrofolate reductase mutants using immobilized methotrexate on agarose beads

FEBS Letters

Volumn 514, Issue 1, 2002, Pages 106-110

  Takahashi, Fumio ^a   Ebihara, Takashi ^a   Mie, Masayasu ^a   Yanagida, Yasuko ^a   Endo, Yaeta ^b   Kobatake, Eiry ^a   Aizawa, Masuo ^a  

^a Tokyo Institute of Technology ^*  (Japan)

^b EHIME UNIVERSITY   (Japan)

Author keywords

Active enzyme mutant; Dihydrofolate reductase; In vitro protein synthesis; Ribosome display

Indexed keywords

AGAROSE; DIHYDROFOLATE REDUCTASE; METHOTREXATE;

BINDING AFFINITY; CELL FREE SYSTEM; CONFERENCE PAPER; ENZYME ACTIVITY; GENE AMPLIFICATION; GENE SEQUENCE; MUTANT; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN SYNTHESIS; RIBOSOME; SEQUENCE ANALYSIS; WHEAT GERM; WILD TYPE;

CELL-FREE SYSTEM; GENE AMPLIFICATION; METHOTREXATE; MUTATION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RIBOSOMES; SEPHAROSE; TETRAHYDROFOLATE DEHYDROGENASE;

TRITICUM AESTIVUM;

EID: 0037029135     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(02)02334-7     Document Type: Conference Paper

References (20)