An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system

Proceedings of the National Academy of Sciences of the United States of America

Volumn 99, Issue 15, 2002, Pages 9715-9720

  Kiga, Daisuke ^a,b   Sakamoto, Kensaku ^c   Kodama, Koichiro ^a,c   Kigawa, Takanori ^a   Matsuda, Takayoshi ^a   Yabuki, Takashi ^a   Shirouzu, Mikako ^a   Harada, Yoko ^a,b   Nakayama, Hiroshi ^d   Takio, Koji ^d   Hasegawa, Yoshinori ^a   Endo, Yaeta ^a,e   Hirao, Ichiro ^a,b,f   Yokoyama, Shigeyuki ^a,b,c  

^a RIKEN YOKOHAMA INSTITUTE   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

^d RIKEN   (Japan)

^e EHIME UNIVERSITY   (Japan)

^f UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; BACTERIAL ENZYME; ENZYME VARIANT; IODOTYROSINE; PROTEIN; TYROSINE; TYROSINE TRANSFER RNA LIGASE;

AMINO ACID METABOLISM; AMINO ACID SUBSTITUTION; AMINOACYLATION; ARTICLE; CELL FREE SYSTEM; CRYSTAL STRUCTURE; ENZYME ENGINEERING; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENETIC RECOMBINATION; MASS SPECTROMETRY; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; RNA TRANSLATION; SITE DIRECTED MUTAGENESIS; WHEAT GERM;

AMINO ACID SUBSTITUTION; AMINO ACIDS; CELL-FREE SYSTEM; DNA, BACTERIAL; ESCHERICHIA COLI; KINETICS; MONOIODOTYROSINE; MUTAGENESIS, SITE-DIRECTED; PROTEIN BIOSYNTHESIS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SEEDS; TRITICUM; TYROSINE; TYROSINE-TRNA LIGASE; VARIATION (GENETICS);

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; GEOBACILLUS STEAROTHERMOPHILUS; PROKARYOTA; TRITICUM AESTIVUM;

EID: 0037162453     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.142220099     Document Type: Article

References (45)