Single-step single-molecule PCR of DNA with a homo-priming sequence using a single primer and hot-startable DNA polymerase

Journal of Bioscience and Bioengineering

Volumn 90, Issue 4, 2000, Pages 456-458

  Nakano, Hideo ^a   Kobayashi, Koei ^a   Ohuchi, Shoji ^a   Sekiguchi, Satoshi ^b   Yamane, Tsuneo ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b Kanagawa 243 0041   (Japan)

Author keywords

PCR; Protein library; Single molecule; Single primer

Indexed keywords

BIOSYNTHESIS; ENZYMES; ESCHERICHIA COLI; PROTEINS;

HOMO-PRIMING SEQUENCES; MUTATIONS; POLYMERASE; PROTEIN LIBRARY;

DNA SEQUENCES;

DNA POLYMERASE;

ARTICLE; DNA DETERMINATION; DNA TEMPLATE; GENE AMPLIFICATION; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN SYNTHESIS;

EID: 0034302304     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(01)80019-0     Document Type: Article

References (13)

1. Filamentous fusion phage: Novel expression vectors that display cloned antigens on the virion surface
2. Screening for receptor ligands using large libraries of peptides linked to the C terminus of the lac repressor
3. Display of heterologous proteins on the surface of microorganisms: From the screening of the combinatorial libraries to live recombinant vaccines
4. An in vitro polysome display system for identifying ligands from very large peptide libraries
5. RNA-peptide fusions for the in vitro selection of peptides and proteins
6. In vitro virus: Bonding of mRNA bearing puromycin at the 3'-terminal end to the C-terminal end of its encoded protein on the ribosome in vitro
7. In vitro method for the generation of protein libraries using PCR amplification of a single DNA molecule and coupled transcription/translation
8. The elimination of primer-dimer accumulation in PCR
9. PCR fidelity of Pfu DNA polymerase and other thermostable DNA polymerases
10. Characterization of DNA polymerase from Pyrococcus sp. strain KOD1 and its application to PCR
11. Digital PCR
12. Modulator-mediated synthesis of active lipase of Pseudomonas sp. 109 by Escherichia coli cell-free coupled transcription/translation system
13. In vitro analysis of roles of a disulfide bridge and a calcium binding site in activation of Pseudomonas sp. strain KWI-56 lipase