Reduction of protein degradation by use of protease-deficient mutants in cell-free protein synthesis system of Escherichia coli

Journal of Bioscience and Bioengineering

Volumn 93, Issue 2, 2002, Pages 151-156

  Jiang, Xiuping ^a   Oohira, Kazuyo ^a   Iwasaki, Yugo ^a   Nakano, Hideo ^a   Ichihara, Shigeyuki ^b   Yamane, Tsuneo ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b MEIJO UNIVERSITY   (Japan)

Author keywords

Cell free protein synthesis; Mutant; Protease; Protein degradation

Indexed keywords

ANTIBIOTICS; DEGRADATION; ELECTROPHORESIS; GENES; PROTEINS; RADIOGRAPHY; SYNTHESIS (CHEMICAL);

PROTEASE-DEFICIENT MUTANTS;

ESCHERICHIA COLI;

BACTERIAL PROTEIN; CARBON 14; DEGP PROTEIN; DODECYL SULFATE SODIUM; ENDOPEPTIDASE LA; GLYCOPROTEIN GP 120; LEUCINE; OMPT PROTEIN; PHOSPHOLIPASE D; POLYACRYLAMIDE; PROTEINASE; UNCLASSIFIED DRUG;

ARTICLE; AUTORADIOGRAPHY; BACTERIAL MUTATION; CELL FREE SYSTEM; DELETION MUTANT; ENZYME DEFICIENCY; ESCHERICHIA COLI; GENE DELETION; GENE MUTATION; GENETIC TRANSCRIPTION; LON GENE; NONHUMAN; OMPT GENE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DEGRADATION; PROTEIN SYNTHESIS; STREPTOMYCES ANTIBIOTICUS; STRUCTURAL GENE;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; STREPTOMYCES; STREPTOMYCES ANTIBIOTICUS; UNCULTURED ACTINOMYCETE;

EID: 0036205202     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(02)80007-X     Document Type: Article

References (22)

1. Subcellular distribution of various proteases in Escherichia coli
2. The DegP and DegQ periplasmic endoproteases of Escherichia coli: Specificity for cleavage sites and substrate conformation
3. Purification and characterization of two novel proteolytic enzymes in membranes of Escherichia coli
4. A novel outer-membrane-associated protease in Escherichia coli
5. An Escherichia coli mutation preventing degradation of abnormal periplasmic proteins
6. ompT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification
7. Regulation by proteolysis: Energy-dependent proteases and their targets
8. ATP hydrolysis-dependent activity of the lon (capR) protein of E. coli K12
9. The product of the lon (capR) gene in Escherichia coli is the ATP-dependent protease, protease La
10. Cell-free production and stable-isotope labeling of milligram quantities of proteins
11. Prolonging cell-free protein synthesis by selective reagent additions
12. Dosage effect of minor arginyl- and isoleucyl-tRNAs on protein synthesis in Escherichia coli in vitro coupled transcription/translation system
13. Isolation and properties of E. coli strains mutant for RNase I
14. The ompB and the regulation of the major outer membrane porin proteins of Escherichia coli K12
15. Crystallization, sequence, and preliminary crystallographic data for an antipeptide fab 50.1 and peptide complexes with the principal neutralizing determinant of HIV-1 gp120
16. Importance of disulfide formation on folding of phospholipase D from Streptomyces antibioticus
17. Characterization of the sppA gene coding for the protease IV, a signal peptide peptidase of Escherichia coli
18. Coupled transcription-translation in prokaryotic cell-free systems
19. In vitro method for the generation of protein liberies using PCR amplification of a single DNA molecule and coupled transcription/translation
20. Crystal structure of a human immunodeficiency virus type 1 neutralizing antibody, 50.1, in complex with its V3 loop peptide antigen
21. A spectrophotometric assay for phospholipase D