Broad network-based predictability of Saccharomyces cerevisiae gene loss-of-function phenotypes

Genome Biology

Volumn 8, Issue 12, 2007, Pages

  McGary, Kriston L ^a   Lee, Insuk ^a   Marcotte, Edward M ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL GROWTH; CELL METABOLISM; CELL SHAPE; CELL STRUCTURE; CONTROLLED STUDY; FUNCTIONAL GENOMICS; GENE DISRUPTION; GENE FUNCTION; GENE LOSS; GENE REGULATORY NETWORK; GENETIC ANALYSIS; GENETIC DISORDER; GENETIC SCREENING; GENETIC VARIABILITY; NONHUMAN; PHENOTYPE; PREDICTION; SACCHAROMYCES CEREVISIAE; YEAST CELL; GENE EXPRESSION REGULATION; GENETICS; GENOMICS; HUMAN; METABOLISM;

SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE PROTEIN;

GENE EXPRESSION REGULATION, FUNGAL; GENE REGULATORY NETWORKS; GENOMICS; HUMANS; PHENOTYPE; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 40449107017     PISSN: 14747596     EISSN: 1474760X     Source Type: Journal    
DOI: 10.1186/gb-2007-8-12-r258     Document Type: Article

References (96)

1. Eisenberg D Marcotte EM Xenarios I Yeates TO Protein function in the post-genomic era. Nature 2000 405 823-826 10.1038/35015694 10866208
2. Hirschhorn JN Daly MJ Genome-wide association studies for common diseases and complex traits. Nat Rev Genet 2005 6 95-108 10.1038/nrg1521 15716906
3. Moffatt MF Kabesch M Liang L Dixon AL Strachan D Heath S Depner M von Berg A Bufe A Rietschel E Genetic variants regulating ORMDL3 expression contribute to the risk of childhood asthma. Nature 2007 448 470-473 10.1038/nature06014 17611496
4. Giaever G Chu AM Ni L Connelly C Riles L Veronneau S Dow S Lucau-Danila A Anderson K Andre B Functional profiling of the Saccharomyces cerevisiae genome. Nature 2002 418 387-391 10.1038/nature00935 12140549
5. Winzeler EA Shoemaker DD Astromoff A Liang H Anderson K Andre B Bangham R Benito R Boeke JD Bussey H Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 1999 285 901-906 10.1126/science.285.5429.901 10436161
6. Kamath RS Fraser AG Dong Y Poulin G Durbin R Gotta M Kanapin A Le Bot N Moreno S Sohrmann M Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature 2003 421 231-237 10.1038/nature01278 12529635
7. Fraser AG Kamath RS Zipperlen P Martinez-Campos M Sohrmann M Ahringer J Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature 2000 408 325-330 10.1038/35042517 11099033
8. Downward J Use of RNA interference libraries to investigate oncogenic signalling in mammalian cells. Oncogene 2004 23 8376-8383 10.1038/ sj.onc.1208073 15517019
9. Marcotte EM Pellegrini M Thompson MJ Yeates TO Eisenberg D A combined algorithm for genome-wide prediction of protein function. Nature 1999 402 83-86 10.1038/47048 10573421
10. Pellegrini M Marcotte EM Thompson MJ Eisenberg D Yeates TO Assigning protein functions by comparative genome analysis: Protein phylogenetic profiles. Proc Natl Acad Sci USA 1999 96 4285-4288 16324 10200254 10.1073/pnas.96.8.4285
11. Bowers PM Pellegrini M Thompson MJ Fierro J Yeates TO Eisenberg D Prolinks: A database of protein functional linkages derived from coevolution. Genome Biol 2004 5 R35 416471 15128449 10.1186/ gb-2004-5-5-r35
12. Myers CL Robson D Wible A Hibbs MA Chiriac C Theesfeld CL Dolinski K Troyanskaya OG Discovery of biological networks from diverse functional genomic data. Genome Biol 2005 6 R114 1414113 16420673 10.1186/ gb-2005-6-13-r114
13. Troyanskaya OG Dolinski K Owen AB Altman RB Botstein D A Bayesian framework for combining heterogeneous data sources for gene function prediction (in Saccharomyces cerevisiae). Proc Natl Acad Sci USA 2003 100 8348-8353 166232 12826619 10.1073/pnas.0832373100
14. von Mering C Huynen M Jaeggi D Schmidt S Bork P Snel B STRING: A database of predicted functional associations between proteins. Nucleic Acids Res 2003 31 258-261 165481 12519996 10.1093/nar/gkg034
15. Mellor JC Yanai I Clodfelter KH Mintseris J DeLisi C Predictome: A database of putative functional links between proteins. Nucleic Acids Res 2002 30 306-309 99135 11752322 10.1093/nar/30.1.306
16. Rhodes DR Tomlins SA Varambally S Mahavisno V Barrette T Kalyana-Sundaram S Ghosh D Pandey A Chinnaiyan AM Probabilistic model of the human protein-protein interaction network. Nat Biotechnol 2005 23 951-959 10.1038/nbt1103 16082366
17. Lee I Date SV Adai AT Marcotte EM A probabilistic functional network of yeast genes. Science 2004 306 1555-1558 10.1126/science.1099511 15567862
18. Fraser AG Marcotte EM Development through the eyes of functional genomics. Curr Opin Genet Dev 2004 14 336-342 10.1016/j.gde.2004.06.015 15261648
19. Sharan R Ulitsky I Shamir R Network-based prediction of protein function. Mol Syst Biol 2007 3 88 1847944 17353930 10.1038/msb4100129
20. Karaoz U Murali TM Letovsky S Zheng Y Ding C Cantor CR Kasif S Whole-genome annotation by using evidence integration in functional-linkage networks. Proc Natl Acad Sci USA 2004 101 2888-2893 365715 14981259 10.1073/pnas.0307326101
21. Schwikowski B Uetz P Fields S A network of protein-protein interactions in yeast. Nat Biotechnol 2000 18 1257-1261 10.1038/82360 11101803
22. Huynen M Snel B Lathe W III Bork P Predicting protein function by genomic context: Quantitative evaluation and qualitative inferences. Genome Res 2000 10 1204-1210 310926 10958638 10.1101/gr.10.8.1204
23. Walker MG Volkmuth W Sprinzak E Hodgson D Klingler T Prediction of gene function by genome-scale expression analysis: Prostate cancer-associated genes. Genome Res 1999 9 1198-1203 310991 10613842 10.1101/gr.9.12.1198
24. Lee I Li Z Marcotte EM An improved, bias-reduced probabilistic functional gene network of Baker's Yeast, Saccharomyces cerevisiae. PLoS ONE 2007 2 e988 1991590 17912365 10.1371/journal.pone.0000988
25. Gandhi TK Zhong J Mathivanan S Karthick L Chandrika KN Mohan SS Sharma S Pinkert S Nagaraju S Periaswamy B Analysis of the human protein interactome and comparison with yeast, worm and fly interaction datasets. Nat Genet 2006 38 285-293 10.1038/ng1747 16501559
26. Hart GT Ramani AK Marcotte EM How complete are current yeast and human protein-interaction networks? Genome Biol 2006 7 120 1794583 17147767 10.1186/gb-2006-7-11-120
27. Jeong H Mason SP Barabasi AL Oltvai ZN Lethality and centrality in protein networks. Nature 2001 411 41-42 10.1038/35075138 11333967
28. Dezso Z Oltvai ZN Barabasi AL Bioinformatics analysis of experimentally determined protein complexes in the yeast Saccharomyces cerevisiae. Genome Res 2003 13 2450-2454 403764 14559778 10.1101/gr.1073603
29. Hart GT Lee I Marcotte EM A high-accuracy map of yeast protein complexes reveals modular basis of gene essentiality. BMC Bioinformatics 2007 8 236 1940025 17605818 10.1186/1471-2105-8-236
30. Willer M Regnacq M Reid PJ Tyson JR Cui W Wilkinson BM Stirling CJ Disruption and functional analysis of six ORFs on chromosome XII of Saccharomyces cerevisiae: YLR124w, YLR125w, YLR126c, YLR127c, YLR128w and YLR129w. Yeast 2000 16 1429-1435 10.1002/ 1097-0061(200011)16:15<1429::AID-YEA629>3.0.CO;2-S 11054824
31. Dwight SS Harris MA Dolinski K Ball CA Binkley G Christie KR Fisk DG Issel-Tarver L Schroeder M Sherlock G Saccharomyces Genome Database (SGD) provides secondary gene annotation using the Gene Ontology (GO). Nucleic Acids Res 2002 30 69-72 99086 11752257 10.1093/nar/30.1.69
32. Askree SH Yehuda T Smolikov S Gurevich R Hawk J Coker C Krauskopf A Kupiec M McEachern MJ A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci USA 2004 101 8658-8663 423251 15161972 10.1073/pnas.0401263101
33. Lesage G Shapiro J Specht CA Sdicu AM Menard P Hussein S Tong AH Boone C Bussey H An interactional network of genes involved in chitin synthesis in Saccharomyces cerevisiae. BMC Genet 2005 6 8 554099 15715908 10.1186/ 1471-2156-6-8
34. Bonangelino CJ Chavez EM Bonifacino JS Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae. Mol Biol Cell 2002 13 2486-2501 117329 12134085 10.1091/mbc.02-01-0005
35. Lesuisse E Knight SA Courel M Santos R Camadro JM Dancis A Genome-wide screen for genes with effects on distinct iron uptake activities in Saccharomyces cerevisiae. Genetics 2005 169 107-122 1448889 15489514 10.1534/genetics.104.035873
36. Markovich S Yekutiel A Shalit I Shadkchan Y Osherov N Genomic approach to identification of mutations affecting caspofungin susceptibility in Saccharomyces cerevisiae. Antimicrob Agents Chemother 2004 48 3871-3876 521896 15388447 10.1128/AAC.48.10.3871-3876.2004
37. Aouida M Page N Leduc A Peter M Ramotar D A genome-wide screen in Saccharomyces cerevisiae reveals altered transport as a mechanism of resistance to the anticancer drug bleomycin. Cancer Res 2004 64 1102-1109 10.1158/0008-5472.CAN-03-2729 14871844
38. Schuldiner M Collins SR Thompson NJ Denic V Bhamidipati A Punna T Ihmels J Andrews B Boone C Greenblatt JF Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile. Cell 2005 123 507-519 10.1016/j.cell.2005.08.031 16269340
39. Kelley R Ideker T Systematic interpretation of genetic interactions using protein networks. Nat Biotechnol 2005 23 561-566 10.1038/nbt1096 15877074
40. Lage K Karlberg EO Storling ZM Olason PI Pedersen AG Rigina O Hinsby AM Tumer Z Pociot F Tommerup N A human phenome-interactome network of protein complexes implicated in genetic disorders. Nat Biotechnol 2007 25 309-316 10.1038/nbt1295 17344885
41. Oti M Snel B Huynen MA Brunner HG Predicting disease genes using protein-protein interactions. J Med Genet 2006 43 691-698 10.1136/ jmg.2006.041376 16611749
42. King OD Lee JC Dudley AM Janse DM Church GM Roth FP Predicting phenotype from patterns of annotation. Bioinformatics 2003 Suppl 1 i183-i189 10.1093/bioinformatics/btg1024
43. Collins SR Kemmeren P Zhao XC Greenblatt JF Spencer F Holstege FC Weissman JS Krogan NJ Towards a comprehensive atlas of the physical interactome of Saccharomyces cerevisiae. Mol Cell Proteomics 2007 6 439-450 17200106
44. Guldener U Munsterkotter M Oesterheld M Pagel P Ruepp A Mewes HW Stumpflen V MPact: The MIPS protein interaction resource on yeast. Nucleic Acids Res 2006 34 D436-441 1347366 16381906 10.1093/nar/gkj003
45. Xenarios I Salwinski L Duan XJ Higney P Kim SM Eisenberg D DIP, the Database of Interacting Proteins: A research tool for studying cellular networks of protein interactions. Nucleic Acids Res 2002 30 303-305 99070 11752321 10.1093/nar/30.1.303
46. Ohya Y Sese J Yukawa M Sano F Nakatani Y Saito TL Saka A Fukuda T Ishihara S Oka S High-dimensional and large-scale phenotyping of yeast mutants. Proc Natl Acad Sci USA 2005 102 19015-19020 1316885 16365294 10.1073/pnas.0509436102
47. Mnaimneh S Davierwala AP Haynes J Moffat J Peng WT Zhang W Yang X Pootoolal J Chua G Lopez A Exploration of essential gene functions via titratable promoter alleles. Cell 2004 118 31-44 10.1016/ j.cell.2004.06.013 15242642
48. Mewes HW Frishman D Mayer KF Munsterkotter M Noubibou O Pagel P Rattei T Oesterheld M Ruepp A Stumpflen V MIPS: Analysis and annotation of proteins from whole genomes in 2005. Nucleic Acids Res 2006 34 D169-D172 1347510 16381839 10.1093/nar/gkj148
49. Robinson MD Grigull J Mohammad N Hughes TR FunSpec: A web-based cluster interpreter for yeast. BMC Bioinformatics 2002 3 35 139976 12431279 10.1186/1471-2105-3-35
50. Saito TL Ohtani M Sawai H Sano F Saka A Watanabe D Yukawa M Ohya Y Morishita S SCMD: Saccharomyces cerevisiae Morphological Database. Nucleic Acids Res 2004 32 D319-D322 308847 14681423 10.1093/nar/gkh113
51. Online Mendelian Inheritance in Man, http://www.ncbi.nlm.nih.gov/omim
52. Scherens B Goffeau A The uses of genome-wide yeast mutant collections. Genome Biol 2004 5 229 463272 15239820 10.1186/gb-2004-5-7-229
53. Network Based Phenotype Prediction http://www.yeastnet.org
54. Kim H Melen K Osterberg M von Heijne G A global topology map of the Saccharomyces cerevisiae membrane proteome. Proc Natl Acad Sci USA 2006 103 11142-11147 1544055 16847258 10.1073/pnas.0604075103
55. Sopko R Huang D Preston N Chua G Papp B Kafadar K Snyder M Oliver SG Cyert M Hughes TR Mapping pathways and phenotypes by systematic gene overexpression. Mol Cell 2006 21 319-330 10.1016/j.molcel.2005.12.011 16455487
56. Gelperin DM White MA Wilkinson ML Kon Y Kung LA Wise KJ Lopez-Hoyo N Jiang L Piccirillo S Yu H Biochemical and genetic analysis of the yeast proteome with a movable ORF collection. Genes Dev 2005 19 2816-2826 1315389 16322557 10.1101/gad.1362105
57. Dennis G Jr Sherman BT Hosack DA Yang J Gao W Lane HC Lempicki RA DAVID: Database for Annotation, Visualization, and Integrated Discovery. Genome Biol 2003 4 P3 10.1186/gb-2003-4-5-p3 12734009
58. Reagan MS Pittenger C Siede W Friedberg EC Characterization of a mutant strain of Saccharomyces cerevisiae with a deletion of the RAD27 gene, a structural homolog of the RAD2 nucleotide excision repair gene. J Bacteriol 1995 177 364-371 176599 7814325
59. Hastings PJ Quah SK von Borstel RC Spontaneous mutation by mutagenic repair of spontaneous lesions in DNA. Nature 1976 264 719-722 10.1038/ 264719a0 796728
60. Giot L Bader JS Brouwer C Chaudhuri A Kuang B Li Y Hao YL Ooi CE Godwin B Vitols E A protein interaction map of Drosophila melanogaster. Science 2003 302 1727-1736 10.1126/science.1090289 14605208
61. Li S Armstrong CM Bertin N Ge H Milstein S Boxem M Vidalain PO Han JD Chesneau A Hao T A map of the interactome network of the metazoan C. elegans. Science 2004 303 540-543 1698949 14704431 10.1126/ science.1091403
62. Rual JF Venkatesan K Hao T Hirozane-Kishikawa T Dricot A Li N Berriz GF Gibbons FD Dreze M Ayivi-Guedehoussou N Towards a proteome-scale map of the human protein-protein interaction network. Nature 2005 437 1173-1178 10.1038/nature04209 16189514
63. Lehner B Fraser AG A first-draft human protein-interaction map. Genome Biol 2004 5 R63 522870 15345047 10.1186/gb-2004-5-9-r63
64. Ramani AK Bunescu RC Mooney RJ Marcotte EM Consolidating the set of known human protein-protein interactions in preparation for large-scale mapping of the human interactome. Genome Biol 2005 6 R40 1175952 15892868 10.1186/gb-2005-6-5-r40
65. Stelzl U Worm U Lalowski M Haenig C Brembeck FH Goehler H Stroedicke M Zenkner M Schoenherr A Koeppen S A human protein-protein interaction network: A resource for annotating the proteome. Cell 2005 122 957-968 10.1016/j.cell.2005.08.029 16169070
66. Joshi-Tope G Gillespie M Vastrik I D'Eustachio P Schmidt E de Bono B Jassal B Gopinath GR Wu GR Matthews L Reactome: A knowledgebase of biological pathways. Nucleic Acids Res 2005 33 D428-432 540026 15608231 10.1093/nar/gki072
67. Franke L Bakel H Fokkens L de Jong ED Egmont-Petersen M Wijmenga C Reconstruction of a functional human gene network, with an application for prioritizing positional candidate genes. Am J Hum Genet 2006 78 1011-1025 1474084 16685651 10.1086/504300
68. Ni L Snyder M A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell 2001 12 2147-2170 55669 11452010
69. Cherry JM Adler C Ball C Chervitz SA Dwight SS Hester ET Jia Y Juvik G Roe T Schroeder M SGD: Saccharomyces Genome Database. Nucleic Acids Res 1998 26 73-79 147204 9399804 10.1093/nar/26.1.73
70. Deane CM Salwinski L Xenarios I Eisenberg D Protein interactions: Two methods for assessment of the reliability of high-throughput observations. Mol Cell Proteomics 2002 1 349-356 10.1074/ mcp.M100037-MCP200 12118076
71. Hu Z Killion PJ Iyer VR Genetic reconstruction of a functional transcriptional regulatory network. Nat Genet 2007 39 683-687 10.1038/ ng2012 17417638
72. Remm M Storm CE Sonnhammer EL Automatic clustering of orthologs and in-paralogs from pairwise species comparisons. J Mol Biol 2001 314 1041-1052 10.1006/jmbi.2000.5197 11743721
73. Shannon P Markiel A Ozier O Baliga NS Wang JT Ramage D Amin N Schwikowski B Ideker T Cytoscape: A software environment for integrated models of biomolecular interaction networks. Genome Res 2003 13 2498-2504 403769 14597658 10.1101/gr.1239303
74. Narayanaswamy R Niu W Scouras AD Hart GT Davies J Ellington AD Iyer VR Marcotte EM Systematic profiling of cellular phenotypes with spotted cell microarrays reveals mating-pheromone response genes. Genome Biol 2006 7 R6 1431703 16507139 10.1186/gb-2006-7-1-r6
75. Birrell GW Giaever G Chu AM Davis RW Brown JM A genome-wide screen in Saccharomyces cerevisiae for genes affecting UV radiation sensitivity. Proc Natl Acad Sci USA 2001 98 12608-12613 60101 11606770 10.1073/ pnas.231366398
76. Huang ME Rio AG Nicolas A Kolodner RD A genomewide screen in Saccharomyces cerevisiae for genes that suppress the accumulation of mutations. Proc Natl Acad Sci USA 2003 100 11529-11534 208792 12972632 10.1073/pnas.2035018100
77. Deutschbauer AM Jaramillo DF Proctor M Kumm J Hillenmeyer ME Davis RW Nislow C Giaever G Mechanisms of haploinsufficiency revealed by genome-wide profiling in yeast. Genetics 2005 169 1915-1925 1449596 15716499 10.1534/genetics.104.036871
78. Chang M Bellaoui M Boone C Brown GW A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage. Proc Natl Acad Sci USA 2002 99 16934-16939 139247 12482937 10.1073/pnas.262669299
79. Zhang J Schneider C Ottmers L Rodriguez R Day A Markwardt J Schneider BL Genomic scale mutant hunt identifies cell size homeostasis genes in S. cerevisiae. Curr Biol 2002 12 1992-2001 10.1016/S0960-9822(02)01305-2 12477387
80. Page N Gerard-Vincent M Menard P Beaulieu M Azuma M Dijkgraaf GJ Li H Marcoux J Nguyen T Dowse T A Saccharomyces cerevisiae genome-wide mutant screen for altered sensitivity to K1 killer toxin. Genetics 2003 163 875-894 1462477 12663529
81. Steinmetz LM Scharfe C Deutschbauer AM Mokranjac D Herman ZS Jones T Chu AM Giaever G Prokisch H Oefner PJ Systematic screen for human disease genes in yeast. Nat Genet 2002 31 400-404 12134146
82. Griffith JL Coleman LE Raymond AS Goodson SG Pittard WS Tsui C Devine SE Functional genomics reveals relationships between the retrovirus-like Ty1 element and its host Saccharomyces cerevisiae. Genetics 2003 164 867-879 1462630 12871900
83. Bennett CB Lewis LK Karthikeyan G Lobachev KS Jin YH Sterling JF Snipe JR Resnick MA Genes required for ionizing radiation resistance in yeast. Nat Genet 2001 29 426-434 10.1038/ng778 11726929
84. Jorgensen P Nishikawa JL Breitkreutz BJ Tyers M Systematic identification of pathways that couple cell growth and division in yeast. Science 2002 297 395-400 10.1126/science.1070850 12089449
85. Blackburn AS Avery SV Genome-wide screening of Saccharomyces cerevisiae to identify genes required for antibiotic insusceptibility of eukaryotes. Antimicrob Agents Chemother 2003 47 676-681 151751 12543677 10.1128/AAC.47.2.676-681.2003
86. Fleming JA Lightcap ES Sadis S Thoroddsen V Bulawa CE Blackman RK Complementary whole-genome technologies reveal the cellular response to proteasome inhibition by PS-341. Proc Natl Acad Sci USA 2002 99 1461-1466 122213 11830665 10.1073/pnas.032516399
87. Desmoucelles C Pinson B Saint-Marc C Daignan-Fornier B Screening the yeast "disruptome" for mutants affecting resistance to the immunosuppressive drug, mycophenolic acid. J Biol Chem 2002 277 27036- 27044 10.1074/jbc.M111433200 12016207
88. Deutschbauer AM Williams RM Chu AM Davis RW Parallel phenotypic analysis of sporulation and postgermination growth in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 2002 99 15530-15535 137751 12432101 10.1073/ pnas.202604399
89. Enyenihi AH Saunders WS Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae. Genetics 2003 163 47-54 1462418 12586695
90. Xie MW Jin F Hwang H Hwang S Anand V Duncan MC Huang J Insights into TOR function and rapamycin response: Chemical genomic profiling by using a high-density cell array method. Proc Natl Acad Sci USA 2005 102 7215- 7220 1091748 15883373 10.1073/pnas.0500297102
91. Chan TF Carvalho J Riles L Zheng XF A chemical genomics approach toward understanding the global functions of the target of rapamycin protein (TOR). Proc Natl Acad Sci USA 2000 97 13227-13232 27207 11078525 10.1073/ pnas.240444197
92. Warringer J Ericson E Fernandez L Nerman O Blomberg A High-resolution yeast phenomics resolves different physiological features in the saline response. Proc Natl Acad Sci USA 2003 100 15724-15729 307635 14676322 10.1073/pnas.2435976100
93. Wilson WA Wang Z Roach PJ Systematic identification of the genes affecting glycogen storage in the yeast Saccharomyces cerevisiae: implication of the vacuole as a determinant of glycogen level. Mol Cell Proteomics 2002 1 232-242 10.1074/mcp.M100024-MCP200 12096123
94. Zewail A Xie MW Xing Y Lin L Zhang PF Zou W Saxe JP Huang J Novel functions of the phosphatidylinositol metabolic pathway discovered by a chemical genomics screen with wortmannin. Proc Natl Acad Sci USA 2003 100 3345-3350 152295 12615994 10.1073/pnas.0530118100
95. Riles L Shaw RJ Johnston M Reines D Large-scale screening of yeast mutants for sensitivity to the IMP dehydrogenase inhibitor 6-azauracil. Yeast 2004 21 241-248 10.1002/yea.1068 14968429
96. Huang RY Eddy M Vujcic M Kowalski D Genome-wide screen identifies genes whose inactivation confer resistance to cisplatin in Saccharomyces cerevisiae. Cancer Res 2005 65 5890-5897 10.1158/0008-5472.CAN-04-4093 15994967