Relevant double mutations in bioengineered Streptomyces clavuligerus deacetoxycephalosporin C synthase result in higher binding specificities which improve penicillin bioconversion

Applied and Environmental Microbiology

Volumn 74, Issue 4, 2008, Pages 1167-1175

  Kian, Sim Goo ^a   Chun, Song Chua ^a   Sim, Tiow Suan ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

AMPICILLIN CONVERSION; CEPHALOSPORIN SYNTHESIS; ENZYME CATALYSIS; SUBSTRATE BINDING AFFINITY;

BACTERIA; BINDING ENERGY; BIOSYNTHESIS; MUTAGENESIS;

ENZYME KINETICS;

ALANINE; AMPICILLIN; ARGININE; CARBENICILLIN; CEFALEXIN; DEACETOXYCEPHALOSPORIN C SYNTHASE; ENZYME; LEUCINE; LYSINE; METHIONINE; PENICILLIN G; PHENETICILLIN; UNCLASSIFIED DRUG;

BACTERIUM; BIOASSAY; BIOTRANSFORMATION; CATALYSIS; ENZYME ACTIVITY; GENETIC ENGINEERING; MUTATION;

ARTICLE; BINDING AFFINITY; BIOASSAY; BIOENGINEERING; BIOTRANSFORMATION; CATALYSIS; ENZYME ACTIVITY; ENZYME MODIFICATION; KINETICS; MUTATION; NUCLEOTIDE SEQUENCE; STREPTOMYCES CLAVULIGERUS;

AMPICILLIN; BASE SEQUENCE; CATALYSIS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COMPUTATIONAL BIOLOGY; DNA PRIMERS; INTRAMOLECULAR TRANSFERASES; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PENICILLIN-BINDING PROTEINS; PENICILLINS; PROTEIN BINDING; PROTEIN ENGINEERING; SEQUENCE ALIGNMENT; STREPTOMYCES; SUBSTRATE SPECIFICITY;

STREPTOMYCES CLAVULIGERUS;

EID: 39649095868     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.02230-07     Document Type: Article

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