Mutation of N304 to leucine in Streptomyces clavuligerus deacetoxycephalosporin C synthase creates an enzyme with increased penicillin analogue conversion

Biochemical and Biophysical Research Communications

Volumn 287, Issue 2, 2001, Pages 507-513

  Chin, Hong Soon ^a   Sim, Janet ^a   Sim, Tiow Suan ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Deacetoxycephalosporin C synthase; Isopenicillin N synthase; Penicillin analogue; Site directed mutagenesis; Superimposition

Indexed keywords

AMOXICILLIN; AMPICILLIN; CEPHALOSPORIN DERIVATIVE; DEACETOXYCEPHALOSPORIN C; DEACETOXYCEPHALOSPORIN C SYNTHASE; ISOPENICILLIN N SYNTHETASE; LEUCINE; LIGAND; PENICILLIN DERIVATIVE; PENICILLIN G; SYNTHETASE; UNCLASSIFIED DRUG;

ARTICLE; BIOTRANSFORMATION; CATALYSIS; ENZYME ACTIVITY; ENZYME BINDING; ENZYME STRUCTURE; GENE MUTATION; HYDROPHOBICITY; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STREPTOMYCES CLAVULIGERUS;

STREPTOMYCES CLAVULIGERUS;

EID: 0035929372     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.5552     Document Type: Article

References (23)

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