Directed evolution of Streptomyces clavuligerus deacetoxycephalosporin C synthase for enhancement of penicillin G expansion

Applied and Environmental Microbiology

Volumn 71, Issue 12, 2005, Pages 8873-8880

  Wei, Chia Li ^a   Yang, Yunn Bor ^b   Deng, Chan Hui ^b   Liu, Wen Chi ^b   Hsu, Jyh Shing ^a,b   Lin, Yu Ching ^a   Liaw, Shwu Huey ^a   Tsai, Ying Chieh ^a  

^a NATIONAL YANG MING UNIVERSITY   (Taiwan)

^b Synmax Biochemical Co Ltd   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTICS; BIOSYNTHESIS; CLONING; DNA; MUTAGENS; RANDOM PROCESSES;

BARREL SUBDOMAIN; MUTANTS; MUTATIONS; SUBDOMAINS;

ENZYMES;

ADICILLIN; ALANINE; BACTERIAL ENZYME; CEPHALOSPORANIC ACID DERIVATIVE; CEPHALOSPORIN DERIVATIVE; CYSTEINE; DEACETOXYCEPHALOSPORIN C SYNTHASE; GLUTAMINE; HISTIDINE; ISOLEUCINE; LEUCINE; METHIONINE; PENICILLIN G; PHENYLACETYL 7 AMINODEACETOXYCEPHALOSPORANIC ACID; THREONINE; TYROSINE; UNCLASSIFIED DRUG; VALINE;

BIOENGINEERING;

ANTIBIOTIC BIOSYNTHESIS; ARTICLE; BACTERIUM MUTANT; CHEMICAL REACTION; DIRECTED MOLECULAR EVOLUTION; DNA MODIFICATION; DRUG MANUFACTURE; ENZYME ACTIVITY; ENZYME ENGINEERING; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN DOMAIN; RING EXPANSION; STREPTOMYCES CLAVULIGERUS; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; DIRECTED MOLECULAR EVOLUTION; INTRAMOLECULAR TRANSFERASES; MUTAGENESIS, SITE-DIRECTED; PENICILLIN G; PENICILLIN-BINDING PROTEINS; PENICILLINS; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; STREPTOMYCES;

BACTERIA (MICROORGANISMS); STREPTOMYCES CLAVULIGERUS;

EID: 29144459217     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.12.8873-8880.2005     Document Type: Article

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