C-terminus modification of Streptomyces clavuligerus deacetoxycephalosporin C synthase improves catalysis with an expanded substrate specificity

Biochemical and Biophysical Research Communications

Volumn 295, Issue 1, 2002, Pages 55-61

  Chin, H S ^a   Sim, T S ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

C terminus; Deacetoxycephalosporin C synthase; Penicillin analogue; Site directed mutagenesis

Indexed keywords

ASPARAGINE; DEACETOXYCEPHALOSPORIN C SYNTHASE; LEUCINE; PENICILLIN DERIVATIVE; SYNTHETASE; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SPECIFICITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STREPTOMYCES CLAVULIGERUS; WILD TYPE;

AMINO ACID SUBSTITUTION; BIOLOGICAL ASSAY; CATALYSIS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HYDROPHOBICITY; INTRAMOLECULAR TRANSFERASES; MUTAGENESIS, SITE-DIRECTED; PENICILLIN-BINDING PROTEINS; PENICILLINS; PROTEIN STRUCTURE, TERTIARY; STREPTOMYCES; SUBSTRATE SPECIFICITY;

STREPTOMYCES; STREPTOMYCES CLAVULIGERUS;

EID: 0036305290     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-291X(02)00629-0     Document Type: Article

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