Environmentally safe production of 7-aminodeacetoxycephalosporanic acid (7-ADCA) using recombinant strains of Acremonium chrysogenum

Nature Biotechnology

Volumn 18, Issue 8, 2000, Pages 857-861

  Velasco, J ^a   Adrio, J L ^a   Moreno, M A ^a   Diez, B ^a   Soler, G ^a   Barredo, J L ^a  

^a Avenida de Antibioóticos 59 61   (Spain)

Author keywords

7 ADCA; CefE; CefEF; Cephalosporin; D amino acid oxidase; Deacetoxycephalosporin C; Glutaryl acylase

Indexed keywords

7 AMINOCEPHALOSPORANIC ACID; AMIDASE; AMINO ACID OXIDASE; BETA LACTAM ANTIBIOTIC; CEPHALOSPORIN DERIVATIVE;

ACREMONIUM; ARTICLE; BIOTRANSFORMATION; DRUG SYNTHESIS; ENZYME ACTIVITY; GENE DISRUPTION; GENETIC TRANSFECTION; NONHUMAN; PENICILLIUM CHRYSOGENUM; PRIORITY JOURNAL; STREPTOMYCES CLAVULIGERUS;

ACREMONIUM; CEPHALOSPORINS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; FERMENTATION; GENES, FUNGAL; MASS SPECTROMETRY; PLASMIDS; RECOMBINATION, GENETIC;

EID: 0033898359     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/78467     Document Type: Article

References (36)

1. The penicillins: Properties, biosynthesis and fermentation
2. Cloning and expression of the fungal expandasehydroxylase gene involved in cephalosporin biosynthesis
3. Isolation of deacetoxycephalosporin C from fermentation broths of Penicillium chrysogenum transformants: Construction of a new fungal biosynthetic pathway
4. Production of deacetoxycephalosporin C by transformants of Penicillium chrysogenum: Antibiotic biosynthetic pathway engineering
5. Cloning and expression of a hybrid Streptomyces clavuligerus cefE gene in Penicillium chrysogenum
6. Improved expression of a hybrid Streptomyces clavuligerus cefE gene in Penicillium chrysogenum
7. Production of cephalosporin intermediates by feeding adipic acid to recombinant Penicillium chrysogenum strains expressing ring expansion activity
8. Incorporation of α,ω-dicarboxylic acids as side chains into the penicillin molecule
9. Partial purification and properties of the α-ketoglutarate-linked ring-expansion enzyme of β-lactam biosynthesis of Cephalosporium acremonium
10. Biochemical characterization and evolutionary implication of β-lactam expandase/hydroxylase, expandase and hydroxylase
11. The substrate specificity of deacetoxycephalosporin C synthase ('expandase') of Streptomyces clavuligerus is extremely narrow
12. The enzymatic ring expansion of penicillins to cephalosporins: Side chain specificity
13. Enzymatic 7-ADCA: I said it couldn't be done
14. Elucidation of conditions allowing conversion of penicillin G and other penicillins to deacetoxycephalosporins by resting cells and extracts of Streptomyces clavuligerus NP1
15. Stimulatory effect of growth in the presence of alcohols on biotransformation of penicillin G into cephalosporin-type antibiotics by resting cells of Streptomyces clavuligerus NP1
16. Construction of a 7-aminocephalosporanic acid (7ACA) biosynthetic operon and direct production of 7ACA in Acremonium chrysogenum
17. New processes for production of 7-aminocephalosporanic acid from Cephalosporium
18. D-amino-acid oxidase gene from Rhodotorula gracilis (Rhodosporidium toruloides) ATCC 26217
19. Engineering the D-amino-acid oxidase from Trigonopsis variabilis to facilitate its overproduction in Escherichia coli and its downstream processing by tailor-made metal chelate supports
20. Molecular cloning and structure of the gene for 7β-(4-carboxybutanamido) cephalosporanic acid acylase from a Pseudomonas strain
21. Process for the enzymatic preparation of 7-aminocephalosporanic acid
22. Enzymatic process for preparing 7-aminocephalosporanic acid and derivatives
23. One-step gene disruption in yeast
24. Molecular characterization and functional analysis in Aspergillus nidulans of the 5'-region of the Penicillium chrysogenum isopenicillin N synthetase gene
25. A reporter gene analysis of penicillin biosynthesis gene expression in Penicillium chrysogenum and its regulation by nitrogen and glucose catabolite repression
26. Expression of the cefG gene is limiting for cephalosporin biosynthesis in Acremonium chrysogenum
27. The NADP-dependent glutamate dehydrogenase gene from Penicillium chrysogenum and the construction of expression vectors for filamentous fungi
28. The β-lactam biosynthesis genes for isopenicillin N epimerase and deacetoxycephalosporin C synthetase are expressed from a single transcript in Streptomyces clavuligerus
29. Preparation of streptomycin
30. A system for genetic transformation of Cephalosporium acremonium
31. Levels of isopenicillin N synthetase and deacetoxycephalosporin C synthetase in Cephalosporium acremonium producing high and low levels of cephalosporin C
32. The cefG gene of Cephalosporium acremonium is linked to the cefEF and encodes a deacetylcephalosporin C acetyltransferase closely related to homoserine O-acetyltransferase
33. Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli
34. Copurification and characterization of deacetoxycephalosporin C synthase/hydroxylase from Cephalosporium acremonium
35. A method for extracting high-molecular-weight deoxyribonucleic acid from fungi