In vitro conversion of penicillin G and ampicillin by recombinant Streptomyces clavuligerus NRRL 3585 deacetoxycephalosporin C synthase

Enzyme and Microbial Technology

Volumn 29, Issue 4-5, 2001, Pages 240-245

  Sim, Janet ^a   Sim, Tiow Suan ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Ampicillin; Ascorbate; Deacetoxycephalosproin C synthase; Dithiothreitol; Penicillin G; Ring expansion

Indexed keywords

ADENOSINETRIPHOSPHATE; CELLS; CHARACTERIZATION; INCLUSIONS;

COFACTORS;

ENZYMES;

2 OXOGLUTARIC ACID; ADENOSINE TRIPHOSPHATE; AMPICILLIN; ASCORBIC ACID; DEACETOXYCEPHALOSPORIN C; DEACETOXYCEPHALOSPORIN C SYNTHASE; DITHIOTHREITOL; FERROUS SULFATE; PENICILLIN G; TROMETAMOL; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME MECHANISM; ENZYME MODIFICATION; IN VITRO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; STREPTOMYCES CLAVULIGERUS;

EID: 0035812391     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(01)00377-5     Document Type: Article

References (23)

1. Cell-free ring expansion of penicillin N to deacetoxycephalosporin C by Cephalosporium acremonium CW-19 and its mutants
2. Conversion of penicillin N to cephalosporin(s) by cell-free extracts of Cephalosporium acremonium
3. Dithiothreitol reactivates deacetoxycephalosporin C synthetase after inactivation
4. Further studies on microbiological ring-expansion of penicillin N
5. Enzymatic approach to synthesis of unnatural beta-lactams
6. The biosynthesis of penicillins and cephalosporins
7. Cephalosporin formation by cell-free extracts from Streptomyces clavuligerus
8. Copurification and characterization of deacetoxycephalosporin C synthetase/hydroxylase from Cephalosporium acremonium
9. Purification and properties of deacetoxycephalosporin C synthase from recombinant Escherichia coli and its comparison with the native enzyme purified from Streptomyces clavuligerus
10. The substrate specificity of deacetoxycephalosporin C synthase ("expandase") of Streptomyces clavuligerus is extremely narrow
11. Purification and characterization of a 2-oxoglutarate-linked ATP-dependent deacetoxycephalosporin C synthase of Streptomyces lactamdurans
12. Elucidation of conditions allowing conversion of penicillin G an other penicillins to deacetoxycephalosporins by resting cells and extracts of Streptomyces clavuligerus NP1
13. Inactivation of deacetoxycephalosporin C synthase in extracts of Streptomyces clavuligerus during bioconversion of penicillin G to deacetoxycephalosporin G
14. Stimulatory effect of growth in the presence of alcohols on biotransformation of penicillin G into cephalosporin-type antibiotics by resting cells of Streptomyces clavuligerus NP1
15. Immobilized Streptomyces clavuligerus NP1 cells for biotransformation of penicillin G into deacetoxycephalosporin G
16. Mutational evidence supporting the involvement of tripartite residues His183, Asp185 and His243 in Streptomyces clavuligerus deacetoxycephalosporin C synthase for catalysis
17. Mutational analysis of tyrosine-191 in the catalysis of Cephalosporium acremonium isopenicillin N synthase
18. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
19. Deacetoxycephalosporin C synthetase: Importance of order of cofactor/reactant addition
20. A rapid, and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
21. Microbiological ring expansion of penicillin N
22. In vivo conversion of penicillin N into a cephalosporin type antibiotic by a non-producing mutant of Streptomyces clavuligerus