The humanization of N-glycosylation pathways in yeast

Nature Reviews Microbiology

Volumn 3, Issue 2, 2005, Pages 119-128

  Wildt, Stefan ^a   Gerngross, Tilllman U ^b  

^a GlycoFi Incorporated   (United States)

^b DARTMOUTH COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ERYTHROPOIETIN; FUNGAL PROTEIN; GLYCOPROTEIN; HEPATITIS B VACCINE; INDUSTRIAL ENZYME; INSULIN; MANNOSE; RECOMBINANT ANTIBODY; RECOMBINANT BETA INTERFERON; RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR; RECOMBINANT PROTEIN; RITUXIMAB; SIALIC ACID; TRASTUZUMAB; VACCINE;

COST BENEFIT ANALYSIS; DRUG DISTRIBUTION; DRUG EFFICACY; DRUG HALF LIFE; DRUG RECEPTOR BINDING; DRUG SYNTHESIS; ENZYME SYNTHESIS; HANSENULA POLYMORPHA; HUMAN; IN VIVO STUDY; KLUYVEROMYCES; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN MODIFICATION; PROTEIN STRUCTURE; REVIEW; SACCHAROMYCES CEREVISIAE;

FUNGAL PROTEINS; GALACTOSE; GENETIC ENGINEERING; GLYCOPROTEINS; GLYCOSYLATION; HUMANS; MANNOSE; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; SIALIC ACIDS; YEASTS;

EID: 13444262282     PISSN: 17401526     EISSN: None     Source Type: Journal    
DOI: 10.1038/nrmicro1087     Document Type: Review

References (62)

1. Nasmyth, K. Eukaryotic gene cloning and expression in yeast. Nature 274, 741-743 (1978).
2. Sudbery, P. E. The expression of recombinant proteins in yeasts. Curr. Opin. Biotechnol. 7, 517-524 (1996).
3. Gellissen, G. & Hollenberg, C. P. Application of yeasts in gene expression studies: a comparison of Saccharomyces cerevisiae, Hansenula polymorpha and Kluyveromyces lactis - a review. Gene 190, 87-97 (1997).
4. Cregg, J. M., Vedvick, T. S. & Raschke, W. C. Recent advances in the expression of foreign genes in Pichia pastoris. Biotechnology 11, 905-910 (1993).
5. Cregg, J. M. & Higgins, D. R. Production of foreign proteins in the yeast Pichia pastoris. Can. J. Bot. 73, S891-S897 (1995).
6. Gellissen, G. & Melber, K. Methylotrophic yeast Hansenula polymorpha as production organism for recombinant pharmaceuticals. Arzneimittelforschung 46, 943-948 (1996).
7. Werten, M. W. T., Van den Bosch, T J., Wind, R. D., Mooibroek, H. & De Wolf, F. A. High-yield secretion of recombinant gelatins by Pichia pastoris. Yeast 15, 1087-1096 (1999).
8. Young, N. M. et al. Structure of the N-linked glycan present on multiple glycoproteins in the Gram-negative bacterium, Campylobacter jejuni. J. Biol. Chem. 277, 42530-42539 (2002).
9. Szymanski, C. M., Yao, R. J., Ewing, C. P., Trust, T. J. & Guerry, P. Evidence for a system of general protein glycosylation in Campylobacter jejuni. Mol. Microbiol. 32, 1022-1030 (1999).
10. Wacker, M. et al. N-linked glycosylation in Campylobacter jejuni and its functional transfer into E. coli. Science 298, 1790-1793 (2002).
11. Thibault, P. et al. Identification of the carbohydrate moieties and glycosylation motifs in Campylobacter jejuni flagellin. J. Biol. Chem. 276, 34862-34870 (2001).
12. McAleer, W. J. et al. Human hepatitis B vaccine from recombinant yeast. Nature 307, 178-180 (1984).
13. Walsh, G. Biopharmaceutical benchmarks - 2003. Nature Biotechnol. 21, 1396 (2003).
14. Humphreys, A. & Boersig, C. Cholesterol drugs dominate. MedAdNews 22, 42-57 (2003).
15. Yuen, C. T., Carr, S. A. & Feizi, T. The spectrum of N linked oligosaccharide structures detected by enzymatic microsequencing on a recombinant soluble CD4 glycoprotein from Chinese hamster ovary cells. Eur. J. Biochem. 192, 523-628 (1990).
16. Ulloa-Aguirre, A., Timossi, C., Barrios-de-Tomasi, J., Maldonado, A. & Nayudu, P. Impact of carbohydrate heterogeneity in function of follicle-stimulating hormone: studies derived from in vitro and in vivo models. Biol. Reprod. 69, 379-389 (2003).
17. Shields, R. L. et al. Lack of fucose on human IgG1 N linked oligosaccharide improves binding to human Fcγ RIII and antibody-dependent cellular toxicity. J. Biol. Chem. 277, 26733-26740 (2002).
18. Gentzsch, M. & Tanner, W. Protein O-glycosylation in yeast: protein-specific mannosyltransferases. Glycobiology 7, 481-486 (1997).
19. Lehle, L. & Bause, E. Primary structural requirements for N-glycosylation and O-glycosyation of yeast mannoproteins. Biochim. Biophys Acta 799, 246-251 (1984).
20. Strahl-Bolsinger, S., Gentzsch, M. & Tanner, W. Protein O mannosylation. Biochim. Biophys. Acta 1426, 297-307 (1999).
21. Ziegler, F. D., Gemmill, T. R. & Trimble, R. B. Glycoprotein synthesis in yeast early events in N-linked oligosaccharide processing in Schizosaccharomyces pombe. J. Biol. Chem. 269, 12527-12535 (1994).
22. Kornfeld, R. & Kornfeld, S. Assembly of asparagine-linked oligosaccharides. Annu. Rev. Biochem. 54, 631-664 (1985).
23. Burda, P & Aebi, M. The dolichol pathway of N-linked glycosylation. Biochim. Biophys. Acta 1426, 239-257 (1999).
24. Gemmill, T. R. & Trimble, R. B. Overview of N- and O linked oligosaccharide structures found in various yeast species. Biochim. Biophys. Acta 1426, 227-237 (1999).
25. Tabas, I. & Kornfeld, S. Purification and characterization of a rat-liver golgi α-mannosidase capable of processing asparagine-linked oligosaccharides. J. Biol. Chem. 254, 1655-1663 (1979).
26. Herscovics, A. Structure and function of class I α1,2-mannosidases involved in glycoprotein synthesis and endoplasmic reticulum quality control. Biochimie 83, 757-762 (2001).
27. Herscovics, A. Importance of glycosidases in mammalian glycoprotein biosynthesis. Biochim. Biophys. Acta 1473, 96-107 (1999).
28. 5 intermediates. J. Biol. Chem. 257, 3660-3668 (1982).
29. Moremen, K. W. & Robbins, P. W. Isolation, characterization, and expression of cDNAs encoding murine α-mannosidase-II, a golgi enzyme that controls conversion of high mannose to complex N-glycans. J. Cell Biol. 115, 1521-1534 (1991).
30. Bendiak, B. & Schachter, H. Control of glycoprotein-synthesis. Kinetic mechanism, substrate-specificity, and inhibition characteristics of UDP-N acetylglucosamine-α-D-mannoside β-1-2 N acetylglucosaminyltransferase-II from rat liver. J. Biol. Chem. 262, 5784-5790 (1987).
31. Schachter, H. The joys of HexNAc. The synthesis and function of N- and O-glycan branches. Glycoconjugate J. 17, 465-483 (2000).
32. Nakanishi-Shindo, Y., Nakayama, K., Tanaka, A., Toda, Y. & Jigami, Y. Structure of the N-linked oligosaccharides that show the complete loss of α-1,6-polymannose outer chain from Och1, Och1 Mnn1, and Och1 Mnn1 Alg3 mutants of Saccharomyces cerevisiae. J. Biol. Chem. 268, 26338-26345 (1993).
33. 2) sugar chains in Saccharomyces cerevisiae. J. Biol. Chem. 273, 26298-26304 (1998).
34. Kalsner, I., Hintz, W., Reid, L. S. & Schachter, H. Insertion into Aspergillus nidulans of functional UDP-GlcNac-α -3-D-mannoside β-1,2-N-acetylglucosaminyl-transferase-I, the enzyme catalyzing the first committed step from oligomannose to hybrid and complex N glycans. Glycoconjugate J. 12, 360-370 (1995).
35. Martinet, W., Maras, M., Saelens, X., Jou, W. M. & Contreras, R. Modification of the protein glycosylation pathway in the methylotrophic yeast Pichia pastoris. Biotechnol. Lett. 20, 1171-1177 (1998).
36. van Vliet, C., Thomas, E. C., Merino-Trigo, A., Teasdale, R. D. & Gleeson, P. A. Intracellular sorting and transport of proteins. Prog. Biophys. Mol. Biol. 83, 1-45 (2003).
37. Gleeson, P. A. Targeting of proteins to the Golgi apparatus. Histochem. Cell Biol. 109, 517-532 (1998).
38. Harter, C. & Wieland, F. The secretory pathway: mechanisms of protein sorting and transport. Biochim. Biophys. Acta 1286, 75-93 (1996).
39. Teasdale, R. D. & Jackson, M. R. Signal-mediated sorting of membrane proteins between the endoplasmic reticulum and the Golgi apparatus. Annu. Rev. Cell Dev. Biol. 12, 27-54 (1996).
40. Pelham, H. R. B., Hardwick, K. G. & Lewis, M. J. Sorting of soluble ER proteins in yeast. EMBO J. 7, 1757-1762 (1988).
41. Munro, S. & Pelham, H. R. B. A C-terminal signal prevents secretion of luminal ER proteins. Cell 48, 899-907 (1987).
42. Dean, N. & Pelham, H. R. B. Recycling of proteins from the Golgi compartment to the ER in yeast. J. Cell Biol. 111, 369-377 (1990).
43. Lewis, M. J., Sweet, D. J. & Pelham, H. R. B. The ERD2 gene determines the specificity of the luminal ER protein retention system. Cell 61, 1359-1363 (1990).
44. Semenza, J. C., Hardwick, K. G., Dean, N. & Pelham, H. R. B. ERD2, a yeast gene required for the receptor-mediated retrieval of luminal ER proteins from the secretory pathway. Cell 61, 1349-1357 (1990).
45. Paulson, J. C. & Colley, K. J. Glycosyltransferases - structure, localization, and control of cell type-specific glycosylation. J. Biol. Chem. 264, 17615-17618 (1989).
46. Krezdorn, C. H. et al. Human β-1,4 galactosyltransferase and α-2,6 sialyltransferase expressed in Saccharomyces cerevisiae are retained as active enzymes in the endoplasmic reticulum. Eur. J. Biochem. 220, 809-817 (1994 ).
47. Schwientek, T, Lorenz, C. & Ernst, J. F. Golgi localization in yeast is mediated by the membrane anchor region of rat-liver sialyltransferase. J. Biol. Chem. 270, 5483-5489 (1995).
48. Yoshida, S. et al. Expression and characterization of rat UDP-N-acetylglucosamine: α-3-D-mannoside β -1,2-N acetylglucosaminyltransferase I in Saccharomyces cerevisiae. Glycobiology 9, 53-58 (1999).
49. Nilsson, T. et al. Kin recognition between medial Golgi enzymes in HeLa cells. EMBO J. 13, 562-574 (1994).
50. Callewaert, N. et al. Use of HDEL-tagged Trichoderma reesei mannosyl oligosaccharide 1,2-α-D-mannosidase for N glycan engineering in Pichia pastoris. FEBS Lett. 503, 173-178 (2001).
51. Bretthauer, R. K. Genetic engineering of Pichia pastoris to humanize N-glycosylation of proteins. Trends Biotechnol. 21, 459-462 (2003).
52. Choi, B. K. et al. Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast Pichia pastoris. Proc. Natl Acad Sci. USA 100, 5022-5027 (2003).
53. 2-PP-Dol mannosyltransferase. Biol. Chem. 382, 321-328 (2001).
54. Aebi, M., Gassenhuber, J., Domdey, H. & Hessen, S. T. Cloning and characterization of the ALG3 gene of Saccharomyces cerevisiae. Glycobiology 6, 439-444 (1996).
55. 2-PP-Dol mannosyltransferase enzyme of P. pastoris. Glycobiology 14, 399-407 (2004).
56. Bobrowicz, P. et al. Engineering of an artificial glycosylation pathway blocked in core oligosaccharide assembly in the yeast Pichia pastoris - production of complex humanized glycoproteins with terminal galactose. Glycobiology 14, 757-766 (2004).
57. Hamilton, S. R. et al. Production of complex human glycoproteins in yeast. Science 301, 1244-1246 (2003).
58. Schwientek, T., Narimatsu, H. & Ernst, J. F. Golgi localization and in vivo activity of a mammalian glycosyltransferase (human β-1,4-galactosyltransferase) in yeast. J. Biol. Chem. 271, 3398-3405 (1996).
59. Vervecken, W. et al. In vivo synthesis of mammalian-like, hybrid-type N-glycans in Pichia pastoris. Appl. Environ. Microbiol. 70, 2639-2646 (2004).
60. Roy, S. K., Yoko-o, T., Ikenaga, H. & Jigami, Y. Functional evidence for UDP-galactose transporter in Saccharomyces cerevisiae through the in vivo galactosylation and in vitro transport assay. J. Biol. Chem. 273, 2583-2590 (1998).
61. Malissard, M., Zeng, S. & Berger, E. G. Expression of functional soluble forms of human β-1,4-galactosyltransferase I, α-2,6-sialyltransferase, and α-1,3-fucosyltransferase VI in the methylotrophic, yeast Pichia pastoris. Biochem. Biophys. Res. Commun. 267, 169-173 (2000).
62. Malissard, M., Zeng, S. & Berger, E. G. The yeast expression system for recombinant glycosyltransferases. Glycoconjugate J. 16, 125-139 (1999).