A simple method to monitor and control methanol feeding of Pichia pastoris fermentations using mid-IR spectroscopy

Journal of Biotechnology

Volumn 128, Issue 2, 2007, Pages 344-353

  Schenk, Jonas ^a   Marison, Ian W ^b   von Stockar, Urs ^a  

^a EPFL   (Switzerland)

^b DUBLIN CITY UNIVERSITY   (Ireland)

Author keywords

Bioprocess control; Methanol concentration; Mid infrared spectroscopy; On line monitoring; Pichia pastoris

Indexed keywords

ABSORPTION; FOURIER TRANSFORM INFRARED SPECTROSCOPY; HIGH PRESSURE LIQUID CHROMATOGRAPHY; INFRARED SPECTROSCOPY; METHANOL; PROTEINS;

BIOPROCESS CONTROL; METHANOL CONCENTRATION; MID-INFRARED SPECTROSCOPY; ON-LINE MONITORING; PICHIA PASTORIS;

YEAST;

METHANOL;

ACCURACY; ANALYTICAL ERROR; ARTICLE; CALIBRATION; CARBON SOURCE; FED BATCH FERMENTATION; FUNGAL STRAIN; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INFRARED SPECTROSCOPY; INSTRUMENT; NONHUMAN; PICHIA PASTORIS; PREDICTION; PRIORITY JOURNAL; PROCESS MONITORING; PROTEIN EXPRESSION;

BIOREACTORS; CALIBRATION; FERMENTATION; METHANOL; PICHIA; SPECTROSCOPY, FOURIER TRANSFORM INFRARED;

PICHIA PASTORIS;

EID: 33846238854     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.09.015     Document Type: Article

References (29)

1. ASTM. Standard Practices for Infrared, Multivariate, Quantitative Analysis (E 1655-97). Annual Book of ASTM Standards (1997), American Society for Testing and Materials, Philadelphia pp. 844-869
2. Brereton R.G. Introduction to multivariate calibration in analytical chemistry. Analyst 125 (2000) 2125-2154
3. Cannizzaro, C, 2002. Spectroscopic monitoring of bioprocesses: a study of carotenoid production by Phaffia rhodozyma yeast. PhD Thesis. EPFL, Lausanne, Switzerland.
4. Cereghino J.L., and Cregg J.M. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24 1 (2000) 45-66
5. Crowley J., McCarthy B., Nunn N.S., Harvey L.M., and McNeil B. Monitoring a recombinant Pichia pastoris fed batch process using Fourier transform mid-infrared spectroscopy (FT-MIRS). Biotechnol. Lett. 22 (2000) 1907-1912
6. Cunha A.E., Clemente J.J., Gomes R., Pinto F., Thomaz M., Miranda S., Pinto F., Moosmayer D., Donner P., and Carrondo M.J.T. Methanol induction optimization for scFv antibody fragment production in Pichia pastoris. Biotechnol. Bioeng. 86 4 (2004) 458-467
7. Curvers S., Linnemann J., Wandrey C., and Takors R. Recombinant protein production with Pichia pastoris in continuous fermentation-kinetic analysis of growth and product formation. Eng. Life Sci. 2 8 (2002) 229-235
8. Doak D.L., and Phillips J.A. In situ monitoring of an Escherichia coli fermentation using a diamond composition ATR probe and mid-infrared spectroscopy. Biotechnol. Prog. 15 (1999) 529-539
9. Hashimoto A., and Kameoka T. Mid-infrared spectroscopic determination of sugar contents in plant-cell culture media using an ATR method. Appl. Spectrosc. 54 7 (2000) 1005-1011
10. Hellwig S., Emde F., Raven N.P.G., Henke M., van der Logt P., and Fischer R. Analysis of single-chain antibody production in Pichia pastoris using on-line methanol control in fed-batch and mixed-feed fermentations. Biotechnol. Bioeng. 74 4 (2001) 344-352
11. Invitrogen. Pichia Fermentation Process Guidelines (2002), Invitrogen, Carlsbad, California
12. Jahic M., Rotticci-Mulder J.C., Martinelle M., Hult K., and Enfors S.O. Modeling of growth and energy metabolism of Pichia pastoris producing a fusion protein. Bioprocess. Biosyst. Eng. 24 (2002) 385-393
13. Jahic M., Wallberg F., Bollock M., Garcia P., and Enfors S.O. Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures. Microb. Cell Fact. 2 (2003) 6
14. Kada G., Falk H., and Gruber H.J. Accurate measurement of avidin and streptavidin in crude biofluids with a new, optimized biotin-fluorescein conjugate. Biochim. Biophys. Acta 1427 (1999) 33-43
15. Kornmann H., Rhiel M., Cannizzaro C., Marison I., and von stockar U. Methodology for real-time, multi-analyte monitoring of fermentations using an in-situ mid-infrared sensor. Biotechnol. Bioeng. 82 6 (2003) 702-709
16. Kornmann H., Valentinotti S., Duboc P., Marison I.W., and von Stockar U. Monitoring and control of Gluconacetobacter xylinus fed-batch cultures using in situ mid-IR spectroscopy. J. Biotechnol. 113 1/3 (2004) 231-245
17. Kornmann H., Valentinotti S., Marison I.W., and von Stockar U. Real-time update of calibration model for better monitoring of batch processes using spectroscopy. Biotechnol. Bioeng. 87 5 (2004) 593-601
18. Martens H., and Naes N. Multivariate Calibration (1998), John Wiley and Sons, New York
19. Minning S., Serrano A., Ferrer P., Solá C., Schmid R.D., and Valero F. Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris. J. Biotechnol. 86 1 (2001) 59-70
20. Navarro-Villoslada F., Perez-Arribas L.V., Leon-Gonzalez M.E., and Polo-Diez L.M. Selection of calibration mixtures and wavelengths for different multivariate calibration methods. Anal. Chim. Acta 313 1/2 (1995) 93-101
21. Pollard D., Buccino R., Connors N., Kirschner T., Olewinski R., Saini K., and Salmon P. Real-time analyte monitoring of a fungal fermentation, at pilot scale, using in situ mid-infrared spectroscopy. Bioprocess. Biosyst. Eng. 24 (2001) 13-24
22. Puxty G., Maeder M., Radack K.P., and Gemperline P.J. Equilibrium modeling of mixtures of methanol and water. Appl. Spectrosc. 59 3 (2005) 329-334
23. Rhiel M.H., Amrhein M.I., Marison I.W., and von Stockar U. The influence of correlated calibration samples on the prediction performance of multivariate models based on mid-Infrared spectra of animal cell cultures. Anal. Chem. 74 20 (2002) 5227-5236
24. Rhiel M.H., Ducommun P., Bolzonella I., Marison I.W., and von Stockar U. Real time in situ monitoring of freely suspended and immobilized cell cultures based on mid-infrared spectroscopic measurements. Biotechnol. Bioeng. 77 2 (2002) 174-185
25. Sreekrishna K., Brankamp R.G., Kropp K.E., Blankenship D.T., Tsay J.T., Smith P.L., Wierschke J.D., Subramaniam A., and Birkenberger L.A. Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris. Gene 190 1 (1997) 55-62
26. Stephanopoulos G.N., Aristidou A.A., and Nielsden J. Metabolic Engineering: Principles and Methodologies (1998), Academic Press, San Diego
27. Stratton J., Chiruvolu V., and Meagher M. High cell-density fermentation. In: Higgins D.R., and Cregg J.M. (Eds). Methods in Molecular Biology (1998), Humana Press, Totowa, NJ 107-120
28. Zhang W., Bevins M.A., Plantz B.A., Smith L.A., and Meagher M.M. Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, serotype A. Biotechnol. Bioeng. 70 1 (2000) 1-8
29. Zocchi A., Jobe A.-M., Neuhaus J.-M., and Ward T.R. Expression and purification of a recombinant avidin with a lowered isoelectric point in Pichia pastoris. Protein Expr. Purif. 32 2 (2003) 167-174