High resolution structure of the large ribosomal subunit from a mesophilic eubacterium

Cell

Volumn 107, Issue 5, 2001, Pages 679-688

  Harms, Joerg ^a   Schluenzen, Frank ^a   Zarivach, Raz ^b   Bashan, Anat ^b   Gat, Sharon ^b   Agmon, Ilana ^b   Bartels, Heike ^a   Franceschi, François ^c   Yonath, Ada ^a,b  

^a MAX PLANCK UNIT FOR STRUCTURAL MOLECULAR BIOLOGY   (Germany)

^b WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^c MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; NUCLEOTIDE; PEPTIDYLTRANSFERASE; RIBOSOME PROTEIN; TRANSFER RNA;

ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; DEINOCOCCUS RADIODURANS; EUBACTERIUM; HALOARCULA MARISMORTUI; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; RIBOSOME SUBUNIT;

BACTERIA (MICROORGANISMS); DEINOCOCCUS; DEINOCOCCUS RADIODURANS; HALOARCULA; HALOARCULA MARISMORTUI; POSIBACTERIA;

EID: 0035977093     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0092-8674(01)00546-3     Document Type: Article

References (62)

1. Methods used in the structure determination of bovine mitochondrial F-1 ATPase
2. Visualization of tRNA movements on the E. coli 70S ribosome during the elongation cycle
3. Localization of L11 protein on the ribosome and elucidation of its involvement in EF-G-dependent translocation
4. The CCP4 suite - Programs for protein crystallography
5. Placement of protein and RNA structures into a 5 Å -Resolution map of the 50S ribosomal subunit
6. The complete atomic structure of the large ribosomal subunit at 2.4 Å resolution
7. The database of ribosomal crosslinks: An update
8. Mechanism of ribosomal peptide bond formation
9. A conformational change in the ribosomal peptidyl transferase center upon active/inactive transition
10. Peptide environment of the peptidyl transferase center from E. coli 70S ribosomes as determined by termoaffinity labeling with dihydrospiramycin
11. The end of the beginning: Structural studies of ribosomal proteins
12. Crystal structure of a conserved ribosomal protein-RNA complex
13. Ribosomes in thiostrepton-resistant mutants of B.megaterium lacking a single 50 S subunit protein
14. The crystal structure of ribosomal protein L14 reveals an important organizational component of the translational apparatus
15. Maximum-likelihood heavy-atom parameter refinement for MIR and MAD methods
16. Structure of ribosomal protein L30 from Thermus thermophilus at 1.9 Å resolution: Conformational flexibility of the molecule
17. Structure of ribosomal protein TL5 complexed with RNA provides new insights into the CTC family of stress proteins
18. Ion metabolism in Halobacterium
19. Ribosomal protein L6: Structural evidence of gene duplication from a primitive RNA binding protein
20. Three-dimensional cryo-electron microscopy localization of EF2 in the Saccharomyces cerevisiae 80S ribosome at 17.5 Å resolution
21. Contributions of basic residues to ribosomal protein L11 recognition of RNA
22. Comparative sequence analysis and the structure of 16S and 23S rRNA
23. 3H-p-azidopuromycin photo affinity labeling of E. coli ribosomes: Evidence for site specific interaction at U2504 and G2502 in domain V of 23S RNA
24. The solution structure of ribosomal protein L36 from Thermus thermophilus reveals a zinc-ribbon-like fold
25. The RNA binding domain of ribosomal protein L11:three-dimensional structure of the RNA-bound form of the protein and its interaction with 23 S rRNA
26. Ribosomal protein L9: A structure determination by the combined use of X-ray crystallography and NMR spectroscopy
27. Improved methods for building protein models in electron density maps and the location of errors in these models
28. Structure of E. coli ribosomal protein L25 complexed with a 5S rRNA fragment at 1.8-A resolution
29. Partial resistance of nascent polypeptide chains to proteolytic digestion due to ribosomal shielding
30. Chloramphenicol resistance mutations in the single 23S rRNA gene of the archaeon Halobacterium halobium
31. Location of exit channel for nascent protein in 80S ribosome
32. Interaction of antibiotics with functional sites in 16S ribosomal RNA
33. The 3D arrangement of the 23 S and 5 S rRNA in E. coli 50 S ribosomal subunit based on a cryo-electron microscopic reconstruction at 7.5 Å resolution
34. Ribosomal protein L5 has a highly twisted concave surface and flexible arms responsible for rRNA binding
35. AMoRe: An automated package for molecular replacement
36. Crystal structure of the RNA binding ribosomal protein L1 from Thermus thermophilus
37. The structural basis of ribosome activity in peptide bond synthesis
38. Structural dynamics of bacterial ribosomes. I. Characterization of vacant couples and their relation to complexed ribosomes
39. Assembly of proteins and 5 S rRNA to transcripts of the major structural domains of 23 S rRNA
40. Processing of X-ray diffraction data collected in oscillation mode
41. Haloarcula marismortui 50S subunit - Complementarity of electron microscopy and X-ray crystallographic information
42. Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3
43. Ribosomal peptidyl transferase can withstand mutations at the putative catalytic nucleotide
44. Mapping important nucleotides in the peptidyl transferase centre of 23S rRNA using a random mutagenesis approach
45. Recognition of the highly conserved GTPase center of 23 S ribosomal RNA by ribosomal protein L11 and the antibiotic thiostrepton
46. Controlled proteolysis of nascent polypeptides in rat liver cell fractions. Location of the polypeptides in rough microsomes
47. Structure of functionally activated small ribosomal subunit at 3.3 angstroms resolution
48. Structural basis for the interaction of chloramphenicol, clindamycin, and macrolides with the peptidyl transferase center in eubacteria
49. Mapping of E. coli ribosomal components involved in peptidyl transferase activity
50. Functional studies on ribosomes lacking protein L1 from mutant E. coli
51. Analysis of mutations at residues A2451 and G2447 of 23S rRNA in the peptidyltransferase active site of the 50S ribosomal subunit
52. The crystal structure of ribosomal protein L22 from Thermus thermophilus: Insights into the mechanism of erythromycin resistance
53. Flexibility, conformational diversity and two dimerization modes in complexes of ribosomal protein L12
54. Comparative cross-linking study on the 50S ribosomal subunit from E. coli
55. Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1
56. A detailed view of a ribosomal active site: The structure of the L11-RNA complex
57. Structure of the 30S ribosomal subunit
58. Architecture of prokaryotic ribosomes
59. Crystal structure of ribosomal protein L4 shows RNA-binding sites for ribosome incorporation and feedback control of the S10 operon
60. Ribosomal protein L27 participates in both 50 S subunit assembly and the peptidyl transferase reaction
61. A tunnel in the large ribosomal subunit revealed by three-dimensional image reconstruction
62. Crystal structure of the ribosome at 5.5 Å resolution