Genetically encoding unnatural amino acids for cellular and neuronal studies

Nature Neuroscience

Volumn 10, Issue 8, 2007, Pages 1063-1072

  Wang, Wenyuan ^a   Takimoto, Jeffrey K ^a   Louie, Gordon V ^a   Baiga, Thomas J ^a   Noel, Joseph P ^a   Lee, Kuo Fen ^b   Slesinger, Paul A ^b   Wang, Lei ^a  

^a SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

^b SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; PEPTIDE; POTASSIUM CHANNEL KV1.4; SYNTHETASE; TRANSFER RNA;

ANIMAL CELL; ARTICLE; CELL FUNCTION; CHEMICAL ANALYSIS; CONTROLLED STUDY; CYTOLOGY; EMBRYO; FEMALE; GENE INACTIVATION; HUMAN; HUMAN CELL; MAMMAL CELL; MOUSE; MUTAGENESIS; NERVE CELL; NEUROBIOLOGY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RAT;

AMINO ACIDS; AMINO ACYL-TRNA SYNTHETASES; ANIMALS; ANIMALS, NEWBORN; CELLS, CULTURED; GENETIC CODE; HIPPOCAMPUS; HUMANS; KV1.4 POTASSIUM CHANNEL; MEMBRANE POTENTIALS; MODELS, BIOLOGICAL; MUTAGENESIS, SITE-DIRECTED; NEURONS; PATCH-CLAMP TECHNIQUES; PROTEIN BIOSYNTHESIS; RATS; RATS, SPRAGUE-DAWLEY; TRANSFECTION;

EID: 34547502435     PISSN: 10976256     EISSN: None     Source Type: Journal    
DOI: 10.1038/nn1932     Document Type: Article

References (38)

1. Callaway, E.M. A molecular and genetic arsenal for systems neuroscience. Trends Neurosci. 28, 196-201 (2005).
2. Giepmans, B.N., Adams, S.R., Ellisman, M.H. & Tsien, R.Y. The fluorescent toolbox for assessing protein location and function. Science 312, 217-224 (2006).
3. Gandhi, C.S. & Isacoff, E.Y. Shedding light on membrane proteins. Trends Neurosci. 28, 472-479 (2005).
4. 2+ based on green fluorescent proteins and calmodulin. Nature 388, 882-887 (1997).
5. Miesenbock, G., De Angelis, D.A. & Rothman, J.E. Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature 394, 192-195 (1998).
6. Nagel, G. et al. Light activation of channelrhodopsin-2 in excitable cells of Caenorhabditis elegans triggers rapid behavioral responses. Curr. Biol. 15, 2279-2284 (2005).
7. Wang, L. & Schultz, P.G. Expanding the genetic code. Angew. Chem. Int. Edn. Engl. 44, 34-66 (2004).
8. Nowak, M.W. et al. Nicotinic receptor binding site probed with unnatural amino acid incorporation in intact cells. Science 268, 439-442 (1995).
9. Lummis, S.C. et al. Cis-trans isomerization at a proline opens the pore of a neurotransmitter-gated ion channel. Nature 438, 248-252 (2005).
10. Lummis, S.C., Beene, D.L., Harrison, N.J., Lester, H.A. & Dougherty, D.A. A cation-pi binding interaction with a tyrosine in the binding site of the GABAC receptor. Chem. Biol. 12, 993-997 (2005).
11. Beene, D.L., Price, K.L., Lester, H.A., Dougherty, D.A. & Lummis, S.C. Tyrosine residues that control binding and gating in the 5-hydroxytryptamine3 receptor revealed by unnatural amino acid mutagenesis. J. Neurosci. 24, 9097-9104 (2004).
12. Wang, L., Brock, A., Herberich, B. & Schultz, P.G. Expanding the genetic code of Escherichia coli. Science 292, 498-500 (2001).
13. Johnston, D. et al. Dendritic potassium channels in hippocampal pyramidal neurons. J. Physiol. (Lond.) 525, 75-81 (2000).
14. + channel. Science 309, 897-903 (2005).
15. Zhou, M., Morais-Cabral, J.H., Mann, S. & MacKinnon, R. Potassium channel receptor site for the inactivation gate and quaternary amine inhibitors. Nature 411, 657-661 (2001).
16. + channel behaves like an open-channel blocker. Neuron 7, 743-753 (1991).
17. Hoshi, T., Zagotta, W.N. & Aldrich, R.W. Biophysical and molecular mechanisms of Shaker potassium channel inactivation. Science 250, 533-538 (1990).
18. Wang, L., Magliery, T.J., Liu, D.R. & Schultz, P.G. A new functional suppressor tRNA/aminoacyl-tRNA synthetase pair for the in vivo incorporation of unnatural amino acids into proteins. J. Am. Chem. Soc. 122, 5010-5011 (2000).
19. Wang, L. & Schultz, P.G. A general approach for the generation of orthogonal tRNAs. Chem. Biol. 8, 883-890 (2001).
20. Galli, G., Hofstetter, H. & Birnstiel, M.L. Two conserved sequence blocks within eukaryotic tRNA genes are major promoter elements. Nature 294, 626-631 (1981).
21. Myslinski, E., Ame, J.C., Krol, A. & Carbon, P. An unusually compact external promoter for RNA polymerase III transcription of the human H1RNA gene. Nucleic Acids Res. 29, 2502-2509 (2001).
22. Edwards, H. & Schimmel, P. A bacterial amber suppressor in Saccharomyces cerevisiae is selectively recognized by a bacterial aminoacyl-tRNA synthetase. Mol. Cell. Biol. 10, 1633-1641 (1990).
23. Doctor, B.P. & Mudd, J.A. Species specificity of amino acid accepter ribonucleic acid and aminoacyl soluble ribonucleic acid synthetases. J. Biol. Chem. 238, 3677-3681 (1963).
24. Drabkin, H.J., Park, H.J. & Rajbhandary, U.L. Amber suppression in mammalian cells dependent upon expression of an Escherichia coli aminoacyl-tRNA synthetase gene. Mol. Cell. Biol. 16, 907-913 (1996).
25. Summerer, D. et al. A genetically encoded fluorescent amino acid. Proc. Natl. Acad. Sci. USA 103, 9785-9789 (2006).
26. Murrell-Lagnado, R.D. & Aldrich, R.W. Interactions of amino terminal domains of Shaker K channels with a pore blocking site studied with synthetic peptides. J. Gen. Physiol. 102, 949-975 (1993).
27. + channel, rat Kv1.4, has two potential domains that could produce rapid inactivation. J. Biol. Chem. 272, 19333-19338 (1997).
28. Antz, C. et al. NMR structure of inactivation gates from mammalian voltage-dependent potassium channels. Nature 385, 272-275 (1997).
29. Wissmann, R. et al. Solution structure and function of the "tandeminactivation domain" of the neuronal A-type potassium channel Kv1.4. J. Biol. Chem. 278, 16142-16150 (2003).
30. Sakamoto, K. et al. Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells. Nucleic Acids Res. 30, 4692-4699 (2002).
31. Paule, M.R. & White, R.J. Survey and summary: transcription by RNA polymerases I and III. Nucleic Acids Res. 28, 1283-1298 (2000).
32. Murphy, S., Di Liegro, C. & Melli, M. The in vitro transcription of the 7SK RNA gene by RNA polymerase III is dependent only on the presence of an upstream promoter. Cell 51, 81-87 (1987).
33. Yuan, Y. & Reddy, R. 5′ flanking sequences of human MRP/7-2 RNA gene are required and sufficient for the transcription by RNA polymerase III. Biochim. Biophys. Acta 1089, 33-39 (1991).
34. Muir, T.W. Semisynthesis of proteins by expressed protein ligation. Annu. Rev. Biochem. 72, 249-289 (2003).
35. Cornish, V.W., Mendel, D. & Schultz, P.G. Probing protein structure and function with an expanded genetic code. Angew. Chem. Int. Edn. Engl. 34, 621-633 (1995).
36. Wang, L., Xie, J. & Schultz, P.G. Expanding the genetic code. Annu. Rev. Biophys. Biomol. Struct. 35, 225-249 (2006).
37. Zufferey, R., Donello, J.E., Trono, D. & Hope, T.J. Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors. J. Virol. 73, 2886-2892 (1999).
38. Chin, J.W. et al. An expanded eukaryotic genetic code. Science 301, 964-967 (2003).