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Volumn 118, Issue 34, 1996, Pages 8150-8151

Site-specific protein modification using a ketone handle

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; FLUORESCENCE; PROTEIN MODIFICATION; REACTION ANALYSIS; TECHNIQUE;

EID: 0029787761     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja961216x     Document Type: Article
Times cited : (153)

References (39)
  • 8
    • 0023006133 scopus 로고
    • (a) Sauer, R. T. et al. Biochemistry 1986, 25, 5992-5998.
    • (1986) Biochemistry , vol.25 , pp. 5992-5998
    • Sauer, R.T.1
  • 17
    • 9544236483 scopus 로고    scopus 로고
    • Unpublished results
    • β-[β-C-carbonyl(N-acetylglucosamine)]-2,3-diaminopropanoic acid) were synthesized as the necessary aminoacyl-pdCpAs and tested for incorporation at sites 44 and 82 in T4L using unnatural amino acid mutagenesis. All three were incorporated with < 1 % efficiency: Cornish, V. W.; Michael, K.; Hoffmann, M.; Kessler, H.; Schultz, P. G. Unpublished results.
    • Cornish, V.W.1    Michael, K.2    Hoffmann, M.3    Kessler, H.4    Schultz, P.G.5
  • 28
    • 9544232000 scopus 로고    scopus 로고
    • note
    • Imine formation with the ∈-amino group of lysine is readily reversible, and the equilibrium lies toward free ketone and amine.
  • 34
    • 9544249003 scopus 로고    scopus 로고
    • note
    • CUA relative to the amount produced from the wt gene in vitro. As a control, the amount of T4L produced when the aminoacyl-tRNA is replaced by an unacylated tRNA is shown to be ≤ 1 %.
  • 36
    • 9544256607 scopus 로고    scopus 로고
    • note
    • 82 → ketone 2 and wt T4L, the protein purification, labeling, and fluorescence experiments were also done at pH 4.5. giving the same results.
  • 37
    • 9544241634 scopus 로고    scopus 로고
    • note
    • 490. A caveat to this quantification method is that the fluorescence of fluorescein is influenced by the exact protein environment.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.