Assessing the in vitro suppressive capacity of regulatory T cells

Immunological Investigations

Volumn 36, Issue 5-6, 2007, Pages 607-628

  Brusko, Todd M ^a   Hulme, Maigan A ^a   Myhr, Courtney B ^a   Haller, Michael J ^a   Atkinson, Mark A ^a,b  

^a UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cell tracking; CFSE; Immune regulation; In vitro suppression assay; PKH26; Regulatory T cells

Indexed keywords

CONTAMINATION; HUMAN; IMMUNOMODULATION; IMMUNOREGULATION; IN VITRO STUDY; LYMPHOCYTE FUNCTION; LYMPHOCYTE PROLIFERATION; METHODOLOGY; NONHUMAN; PRIORITY JOURNAL; PURIFICATION; REGULATORY T LYMPHOCYTE; REVIEW; T LYMPHOCYTE ACTIVATION;

ANIMALS; AUTOIMMUNITY; CELL DEATH; HUMANS; T-LYMPHOCYTES, REGULATORY;

EID: 37649012475     PISSN: 08820139     EISSN: 15324311     Source Type: Journal    
DOI: 10.1080/08820130701790368     Document Type: Review

References (51)

1. Allakhverdi, Z., Fitzpatrick, D., Boisvert, A., Baba, N., Bouguermouh, S., Sarfati, M., and Delespesse, G. (2006). Expression of CD103 identifies human regulatory T-cell subsets. J Allergy Clin Immunol 118: 1342-1349.
2. Annunziato, F., Cosmi, L., Liotta, F., Lazzeri, E., Manetti, R., Vanini, V., Romagnani, P., Maggi, E., and Romagnani, S. (2002). Phenotype, localization, and mechanism of suppression of CD4(+)CD25(+) human thymocytes. JExpMed 196, 379-387.
3. Bach, J. F. (2003). Autoimmune diseases as the loss of active "self-control7quot;. AnnNYAcadSci 998: 161-177.
4. Baecher-Allan, C., Brown, J. A., Freeman, G. J., and Hafler, D. A. (2001). CD4+CD25high regulatory cells in human peripheral blood. JImmunol 167: 1245-1253.
5. Baecher-Allan, C., Brown, J. A., Freeman, G. J., and Hafler, D. A. (2003). CD4+CD25+ regulatory cells from human peripheral blood express very high levels of CD25 ex vivo. NovartisFoundSymp 252: 67-88.
6. Baecher-Allan, C., Viglietta, V., and Hafler, D. A. (2004). Human CD4+CD25+ regulatory T cells. SeminImmunol 16: 89-98.
7. Baecher-Allan, C., Wolf, E., and Hafler, D. A. (2006). MHC class II expression identifies functionally distinct human regulatory T cells. J Immunol 176: 4622-4631.
8. Balandina, A., Lecart, S., Dartevelle, P., Saoudi, A., and Berrih-Aknin, S. (2005). Functional defect of regulatory CD4(+)CD25+ T cells in the thymus of patients with autoimmune myasthenia gravis. Blood 105: 735-741.
9. Battaglia, M., Stabilini, A., and Roncarolo, M. G. (2005). Rapamycin selectively expands CD4+CD25+FoxP3+ regulatory T cells. Blood 105: 4743-4748.
10. Bennett, C. L., Christie, J., Ramsdell, F., Brunkow, M. E., Ferguson, P. J., Whitesell, L., Kelly, T. E., Saulsbury, F. T., Chance, P. F., and Ochs, H. D. (2001). The immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) is caused by mutations of FOXP3. NatGenet 27: 20-21.
11. Billiard, F., Litvinova, E., Saadoun, D., Djelti, F., Klatzmann, D., Cohen, J. L., Marodon, G., and Salomon, B. L. (2006). Regulatory and effector T cell activation levels are prime determinants of in vivo immune regulation. J Immunol 177: 2167-2174.
12. Bluestone, J. A. (1997). Is CTLA-4 a master switch for peripheral T cell tolerance? JImmunol 158: 1989-1993.
13. Bluestone, J. A., and Tang, Q. (2004). Therapeutic vaccination using CD4+CD25+ antigen-specific regulatory T cells. ProcNatlAcadSciUSA. 101: Suppl 2: 14622-6.
14. Brunkow, M. E., Jeffery, E. W., Hjerrild, K. A., Paeper, B., Clark, L. B., Yasayko, S. A., Wilkinson, J. E., Galas, D., Ziegler, S. F., and Ramsdell, F. (2001). Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse. NatGenet 27, 68-73.
15. Brusko, T., Wasserfall, C., McGrail, K., Schatz, R., Viener, H. L., Schatz, D., Haller, M., Rockell, J., Gottlieb, P., Clare-Salzler, M., and Atkinson, M. (2007). No alterations in the frequency of FOXP3+ regulatory T-cells in type 1 diabetes. Diabetes 56: 604-612.
16. Brusko, T. M., Wasserfall, C. H., Clare-Salzler, M. J., Schatz, D. A., and Atkinson, M. A. (2005). Functional defects and the influence of age on the frequency of CD4+ CD25+ T-cells in type 1 diabetes. Diabetes 54: 1407-1414.
17. Deaglio, S., Dwyer, K. M., Gao, W., Friedman, D., Usheva, A., Erat, A., Chen, J. F., Enjyoji, K., Linden, J., Oukka, M., et al. (2007). Adenosine generation catalyzed by CD39 and CD73 expressed on regulatory T cells mediates immune suppression. J Exp Med. 204(6): 1257-1265.
18. Duggleby, R. C., Shaw, T. N., Jarvis, L. B., Kaur, G., and Hill Gaston, J. S. (2007). CD27 expression discriminates between regulatory and non-regulatory cells after expansion of human peripheral blood CD4(+) CD25(+) cells. Immunology 121: 129-139.
19. Eddahri, F., Oldenhove, G., Denanglaire, S., Urbain, J., Leo, O., and Andris, F. (2006). CD4+ CD25+ regulatory T cells control the magnitude of T-dependent humoral immune responses to exogenous antigens. Eur J Immunol 36: 855-863.
20. Fontenot, J. D., Gavin, M. A., and Rudensky, A. Y. (2003). Foxp3 programs the development and function of CD4+CD25+ regulatory T cells. NatImmunol 4: 330-336.
21. Fontenot, J. D., Rasmussen, J. P., Gavin, M. A., and Rudensky, A. Y. (2005). A function for interleukin 2 in Foxp3-expressing regulatory T cells. Nat Immunol 6: 1142-1151.
22. Givan, A. L., Fisher, J. L., Waugh, M. G., Ernstoff, M. S., Wallace, P. K. (1999). A flow cytometric method to estimate the precursor frequencies of cells proliferating in response to specific antigens. J. Immunol. Methods 230(1-2):99-112.
23. Hoffmann, P., Eder, R., Boeld, T. J., Doser, K., Piseshka, B., Andreesen, R., and Edinger, M. (2006). Only the CD45RA+ subpopulation of CD4+CD25high T cells gives rise to homogeneous regulatory T-cell lines upon in vitro expansion. Blood 108: 4260-4267.
24. Hoffmann, P., Eder, R., Kunz-Schughart, L. A., Andreesen, R., and Edinger, M. (2004). Large-scale in vitro expansion of polyclonal human CD4(+)CD25high regulatory T cells. Blood 104: 895-903.
25. Huan, J., Culbertson, N., Spencer, L., Bartholomew, R., Burrows, G. G., Chou, Y. K., Bourdette, D., Ziegler, S. F., Offner, H., and Vandenbark, A. A. (2005). Decreased FOXP3 levels in multiple sclerosis patients. J Neurosci Res 81: 45-52.
26. June, C. H., and Blazar, B. R. (2006). Clinical application of expanded CD4+25+ cells. Semin Immunol 18: 78-88.
27. Kamanaka, M., Kim, S. T., Wan, Y. Y., Sutterwala, F. S., Lara-Tejero, M., Galan, J. E., Harhaj, E., and Flavell, R. A. (2006). Expression of interleukin-10 in intestinal lymphocytes detected by an interleukin-10 reporter knockin tiger mouse. Immunity 25: 941-952.
28. Korn, T., Bettelli, E., Gao, W., Awasthi, A., Jager, A., Strom, T. B., Oukka, M., and Kuchroo, V. K. (2007). IL-21 initiates an alternative pathway to induce proinflammatory T(H)17 cells. Nature 448: 484-487.
29. Levings, M. K., Allan, S., d'Hennezel, E., and Piccirillo, C. A. (2006). Functional dynamics of naturally occurring regulatory T cells in health and autoimmunity. Adv Immunol 92: 119-155.
30. Liu, W., Putnam, A. L., Xu-Yu, Z., Szot, G. L., Lee, M. R., Zhu, S., Gottlieb, P. A., Kapranov, P., Gingeras, T. R., de St Groth, B. F., et al. (2006). CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells. J Exp Med 203: 1701-1711.
31. McHugh, R. S., Whitters, M. J., Piccirillo, C. A., Young, D. A., Shevach, E. M., Collins, M., and Byrne, M. C. (2002). CD4(+)CD25(+) immunoregulatory T cells: gene expression analysis reveals a functional role for the glucocorticoid-induced TNF receptor. Immunity 16: 311-323.
32. Mempel, T. R., Pittet, M. J., Khazaie, K., Weninger, W., Weissleder, R., von Boehmer, H., and von Andrian, U. H. (2006). Regulatory T cells reversibly suppress cytotoxic T cell function independent of effector differentiation. Immunity 25: 129-141.
33. Pasare, C., and Medzhitov, R. (2003). Toll pathway-dependent blockade of CD4+CD25+ T cell-mediated suppression by dendritic cells. Science 299: 1033-1036.
34. Piccirillo, C. A., and Shevach, E. M. (2004). Naturally-occurring CD4+CD25+ immunoregulatory T cells: central players in the arena of peripheral tolerance. SeminImmunol 16: 81-88.
35. Roncarolo, M. G., Gregori, S., Battaglia, M., Bacchetta, R., Fleischhauer, K., and Levings, M. K. (2006). Interleukin-10-secreting type 1 regulatory T cells in rodents and humans. Immunol Rev 212: 28-50.
36. Sakaguchi, S. (2004). Naturally arising CD4+ regulatory t cells for immunologic self-tolerance and negative control of immune responses. AnnuRevImmunol 22: 531-562.
37. Sakaguchi, S., Sakaguchi, N., Asano, M., Itoh, M., and Toda, M. (1995). Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor alpha-chains (CD25). Breakdown of a single mechanism of self-tolerance causes various autoimmune diseases. JImmunol 155: 1151-1164.
38. Seddiki, N., Santner-Nanan, B., Martinson, J., Zaunders, J., Sasson, S., Landay, A., Solomon, M., Selby, W., Alexander, S. I., Nanan, R., et al. (2006). Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells. J Exp Med 203: 1693-1700.
39. Shevach, E. M. (2002). CD4+ CD25+ suppressor T cells: more questions than answers. NatRevImmunol 2: 389-400.
40. Shimizu, J., Yamazaki, S., Takahashi, T., Ishida, Y., and Sakaguchi, S. (2002). Stimulation of CD25(+)CD4(+) regulatory T cells through GITR breaks immunological self-tolerance. NatImmunol 3: 135-142.
41. Shultz, L. D., Ishikawa, F., and Greiner, D. L. (2007). Humanized mice in translational biomedical research. Nat Rev Immunol 7: 118-130.
42. Strauss, L., Whiteside, T. L., Knights, A., Bergmann, C., Knuth, A., and Zippelius, A. (2007). Selective survival of naturally occurring human CD4+CD25+Foxp3+ regulatory T cells cultured with rapamycin. J Immunol 178: 320-329.
43. Taams, L. S., Boot, E. P., van Eden, W., and Wauben, M. H. (2000). 'Anergic' T cells modulate the T-cell activating capacity of antigen-presenting cells. J Autoimmun 14: 335-341.
44. Tang, Q., Adams, J. Y., Tooley, A. J., Bi, M., Fife, B. T., Serra, P., Santamaria, P., Locksley, R. M., Krummel, M. F., and Bluestone, J. A. (2006). Visualizing regulatory T cell control of autoimmune responses in nonobese diabetic mice. Nat Immunol 7: 83-92.
45. Tang, Q., and Krummel, M. F. (2006). Imaging the function of regulatory T cells in vivo. Curr Opin Immunol 18: 496-502.
46. Thornton, A. M., and Shevach, E. M. (1998). CD4+CD25+ immunoregulatory T cells suppress polyclonal T cell activation in vitro by inhibiting interleukin 2 production. JExpMed 188: 287-296.
47. Thornton, A. M., and Shevach, E. M. (2000). Suppressor effector function of CD4+CD25+ immunoregulatory T cells is antigen nonspecific. JImmunol 164: 183-190.
48. Tran, D. Q., Ramsey, H., and Shevach, E. M. (2007). Induction of FOXP3 expression in naive human CD4+FOXP3- T cells by T cell receptor stimulation is TGF{beta}-dependent but does not confer a regulatory phenotype. Blood. (e-pub ahead of print).
49. Venken, K., Thewissen, M., Hellings, N., Somers, V., Hensen, K., Rummens, J. L., and Stinissen, P. (2007). A CFSE based assay for measuring CD4+CD25+ regulatory T cell mediated suppression of auto-antigen specific and polyclonal T cell responses. J Immunol Methods 322: 1-11.
50. Weiner, H. L. (2001). Induction and mechanism of action of transforming growth factor-beta-secreting Th3 regulatory cells. Immunol Rev 182: 207-214.
51. Ziegler, S. F. (2006). FOXP3: of mice and men. Annu Rev Immunol 24: 209-226.