A flow cytometric method to estimate the precursor frequencies of cells proliferating in response to specific antigens

Journal of Immunological Methods

Volumn 230, Issue 1-2, 1999, Pages 99-112

  Givan, Alice L ^a   Fisher, Jan L ^a   Waugh, Mary ^a   Ernstoff, Marc S ^a   Wallace, Paul K ^a  

^a DARTMOUTH MEDICAL SCHOOL   (United States)

Author keywords

Flow cytometry; Limiting dilution; Membrane dye; Precursor frequency; Proliferation

Indexed keywords

FLUORESCENT DYE;

ANIMAL CELL; ARTICLE; CELL PROLIFERATION; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN EXPERIMENT; NORMAL HUMAN; PRIORITY JOURNAL; T LYMPHOCYTE ACTIVATION;

ANTIGENS; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; CELL DIFFERENTIATION; CELL DIVISION; EVALUATION STUDIES; FLOW CYTOMETRY; FLUORESCENT DYES; HUMANS; IMMUNOLOGIC TECHNIQUES; IMMUNOPHENOTYPING; LYMPHOCYTE ACTIVATION; ORGANIC CHEMICALS; T-LYMPHOCYTES; TETANUS TOXOID;

EID: 0032711598     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(99)00136-2     Document Type: Article

References (29)

1. Allsopp C.E.M., Nicholls S.J., Langhorne J. A flow cytometric method to assess antigen-specific proliferative responses of different subpopulations of fresh and cryopreserved human peripheral blood mononuclear cells. J. Immunol. Methods. 214:1998;175.
2. Altman J.D., Moss P.A.H., Goulder P.J.R., Barouch D.H., McHeyzer-Williams M.G., Bell J.I., McMichael A.J., Davis M.M. Phenotypic analysis of antigen-specific T lymphocytes. Science. 274:1996;94.
3. Bagwell, C.B., 1993. Theoretical aspects of data analysis. In: Bauer, K.D., Duque, R.E., Shankey, T.V. (Eds.), Clinical Flow Cytometry. Williams and Wilkins, Baltimore, p. 41.
4. Bird J.J., Brown D.R., Mullen A.C., Moskowitz N.H., Mahowald M.A., Sider J.R., Gajewski T.F., Wang C.-R., Reiner S.L. Helper T cell differentiation is controlled by the cell cycle. Immunity. 9:1998;229.
5. Brett S.J., Kingston A.E., Colston M.J. Limiting dilution analysis of the human T cell response to mycobacterial antigens from BCG vaccinated individuals and leprosy patients. Clin. Exp. Immunol. 68:1987;510.
6. Callan M.F.C., Tan L., Annels N., Ogg G.S., Wilson J.D.K., O'Callaghan C.A., Steven N., McMichael A.J., Rickinson A.B. Direct visualization of antigen-specific CD8+T cells during the primary immune response to Epstein-Barr virus in vivo. J. Exp. Med. 187:1998;1395.
7. Cupp J.E., Leary J.F., Cernichiari E., Wood J.C., Doherty R.A. Rare-event analysis methods for detection of fetal red blood cells in maternal blood. Cytometry. 5:1984;138.
8. Dórea R.C.C., Coutinho S.G., Sabroza P.C., Hendriks J., Cysne L., Conceição-Silva F. Quantification of Leishmania-specific T cells in human American cutaneous leishmaniasis (Leishmania braziliensis braziliensis) by limiting dilution. Clin. Exp. Immunol. 71:1988;26.
9. ElGhazali G.E.B., Paulie S., Andersson G., Hansson Y., Holmquist G., Sun J.-B., Olsson T., Ekre H.P., Troye-Blomberg M. Number of interleukin-4- and interferon-g-secreting human T cells reactive with tetanus toxoid and the mycobacterial antigen PPD or phytohemagglutinin: distinct response profiles depending on the type of antigen used for activation. Eur. J. Immunol. 23:1993;2740.
10. Gross H.J., Verwer B., Houck D., Recktenwald D. Detection of rare cells at a frequency of one per million by flow cytometry. Cytometry. 14:1993;519.
11. Hasbold J., Lyons A.B., Kehry M.R., Hodgkin P.D. Cell division number regulates IgG1 and IgE switching of B cells following stimulation by CD40 ligand and IL-4. Eur. J. Immunol. 28:1998;1040.
12. Horan P.K., Slezak S.E. Stable cell membrane labeling. Nature. 340:1989;167.
13. Horan P.K., Slezak S.E., Jensen B.D. Cellular proliferation history by fluorescent analysis. Cytometry. Suppl. 2:1988;38. abstract.
14. Kurts C., Carbone F.R., Barnden M., Blanas E., Allison J., Heath W.R., Miller J.F.A.P. CD4+T cell help impairs CD8+T cell deletion induced by cross-presentation of self-antigens and favors autoimmunity. J. Exp. Med. 186:1997;2057.
15. Kurts C., Kosaka H., Carbone F.R., Miller J.F.A.P., Heath W.R. ClassI-restricted cross-presentation of exogenous self-antigens leads to deletion of autoreactive CD8+T cells. J. Exp. Med. 186:1997;239.
16. Lefkovits I. Induction of antibody forming cell clones in micro cultures. Eur. J. Immunol. 2:1972;360.
17. Lefkovits, I., Waldmann, H., 1999. Limiting Dilution Analysis of Cells in the Immune System. Cambridge Univ. Press, Cambridge.
18. Lovett-Racke A.E., Trotter J.L., Lauber J., Perrin P.J., June C.H., Racke M.K. Decreased dependence of myelin basic protein-reactive T cells on CD28-mediated costimulation in multiple sclerosis patients. J. Clin. Invest. 101:1998;725.
19. Lyons A.B., Parish C.R. Determination of lymphocyte division by flow cytometry. J. Immunol. Methods. 171:1994;131.
20. McCutcheon M., Wehner N., Wensky A., Kushner M., Doan S., Hsiao L., Calabresi P., Ha T., Tran T.V., Tate K.M., Wenkelhake J., Spack E.G. A sensitive ELISPOT assay to detect low-frequency human T lymphocytes. J. Immunol. Methods. 210:1997;149.
21. Ogg G.S., Jin X., Bonhoeffer S., Dunbar P.R., Nowak M.A., Monard S., Segal J.P., Cao Y., Rowland-Jones S.L., Cerundolo V., Hurley A., Markowitz M., Ho D.D., Nixon D.F., McMichael A.J. Quantitation of HIV-1-specific cytotoxic T lymphocytes and plasma load of viral RNA. Science. 279:1998;2103.
22. O'Gorman M.R.G., Corrochano V., Poon R.Y.M. Beyond tritiated thymidine: flow cytometric assays for the evaluation of lymphocyte activation/proliferation. Clin. Immunol. Newsl. 16:1996;164.
23. Plebanski M., Burtles S.S. In vitro primary responses of human T cells to soluble protein antigens. J. Immunol. Methods. 170:1994;15.
24. Sharrock C.E.M., Kaminski E., Man S. Limiting dilution analysis of human T cells: a useful clinical tool. Immunol. Today. 11:1990;281.
25. Song H.K., Noorchashm H., Lieu Y.K., Roastami S., Greeley S.A.S., Barker C.F., Naji A. Alloimmune responses against major and minor histocompatibility antigens: distinct division kinetics and requirement for CD28 costimulation. J. Immunol. 162:1999;2467.
26. Tan L.C., Gudgeon N., Annels N.E., Hansasuta P., O'Callaghan C.A., Rowland-Jones S., McMichael A.J., Rickinson A.B., Callan M.F.C. A re-evaluation of the frequency of CD8+ T cells specific for EBV in healthy virus carriers. J. Immunol. 162:1999;1827.
27. Tough D.F., Sprent J. Anti-viral immunity: spotting virus-specific T cells. Curr. Biol. 8:1998;R498.
28. van Oers M.H.J., Pinkster J., Zeijlemaker W.P. Quantification of antigen-reactive cells among human T lymphocytes. Eur. J. Immunol. 8:1978;477.
29. Wells A.D., Gudmundsdottir H., Turka L.A. Following the fate of individual T cells throughout activation and clonal expansion. J. Clin. Invest. 100:1997;3173.