-
1
-
-
33947500247
-
Chemical tools for biomolecular imaging
-
Johnsson N., and Johnsson K. Chemical tools for biomolecular imaging. ACS Chem Biol 2 (2007) 31-38
-
(2007)
ACS Chem Biol
, vol.2
, pp. 31-38
-
-
Johnsson, N.1
Johnsson, K.2
-
2
-
-
0032503999
-
Specific covalent labeling of recombinant protein molecules inside live cells
-
Griffin B.A., Adams S.R., and Tsien R.Y. Specific covalent labeling of recombinant protein molecules inside live cells. Science 281 (1998) 269-272
-
(1998)
Science
, vol.281
, pp. 269-272
-
-
Griffin, B.A.1
Adams, S.R.2
Tsien, R.Y.3
-
3
-
-
3242754218
-
Specific labeling of cell surface proteins with chemically diverse compounds
-
George N., Pick H., Vogel H., Johnsson N., and Johnsson K. Specific labeling of cell surface proteins with chemically diverse compounds. J Am Chem Soc 126 (2004) 8896-8897
-
(2004)
J Am Chem Soc
, vol.126
, pp. 8896-8897
-
-
George, N.1
Pick, H.2
Vogel, H.3
Johnsson, N.4
Johnsson, K.5
-
4
-
-
3042546498
-
Labeling proteins with small molecules by site-specific posttranslational modification
-
Yin J., Liu F., and Walsh C.T. Labeling proteins with small molecules by site-specific posttranslational modification. J Am Chem Soc 126 (2004) 7754-7755
-
(2004)
J Am Chem Soc
, vol.126
, pp. 7754-7755
-
-
Yin, J.1
Liu, F.2
Walsh, C.T.3
-
5
-
-
0037225952
-
A general method for the covalent labeling of fusion proteins with small molecules in vivo
-
Keppler A., Gendreizig S., Gronemeyer T., Pick H., Vogel H., and Johnsson K. A general method for the covalent labeling of fusion proteins with small molecules in vivo. Nat Biotechnol 21 (2003) 86-89
-
(2003)
Nat Biotechnol
, vol.21
, pp. 86-89
-
-
Keppler, A.1
Gendreizig, S.2
Gronemeyer, T.3
Pick, H.4
Vogel, H.5
Johnsson, K.6
-
6
-
-
9444247617
-
Short tetracysteine tags to beta-tubulin demonstrate the significance of small labels for live cell imaging
-
Andresen M., Schmitz-Salue R., and Jakobs S. Short tetracysteine tags to beta-tubulin demonstrate the significance of small labels for live cell imaging. Mol Biol Cell 15 (2004) 5616-5622
-
(2004)
Mol Biol Cell
, vol.15
, pp. 5616-5622
-
-
Andresen, M.1
Schmitz-Salue, R.2
Jakobs, S.3
-
7
-
-
20844434337
-
A FlAsH-based FRET approach to determine G protein-coupled receptor activation in living cells
-
GPCR-TC-CFP fusion proteins are functional receptors for ligand binding and downstream signaling. FlAsH labeling of these proteins allowed the detection of conformational changes on agonist or antagonist binding by intramolecular FRET between FlAsH and CFP.
-
Hoffmann C., Gaietta G., Bunemann M., Adams S.R., Oberdorff-Maass S., Behr B., Vilardaga J.P., Tsien R.Y., Eisman M.H., and Lohse M.J. A FlAsH-based FRET approach to determine G protein-coupled receptor activation in living cells. Nat Meth 2 (2005) 171-176. GPCR-TC-CFP fusion proteins are functional receptors for ligand binding and downstream signaling. FlAsH labeling of these proteins allowed the detection of conformational changes on agonist or antagonist binding by intramolecular FRET between FlAsH and CFP.
-
(2005)
Nat Meth
, vol.2
, pp. 171-176
-
-
Hoffmann, C.1
Gaietta, G.2
Bunemann, M.3
Adams, S.R.4
Oberdorff-Maass, S.5
Behr, B.6
Vilardaga, J.P.7
Tsien, R.Y.8
Eisman, M.H.9
Lohse, M.J.10
-
8
-
-
31144458770
-
Oscillations of cyclic AMP in hormone-stimulated insulin-secreting beta-cells
-
Dyachok O., Isakov Y., Sagetorp J., and Tengholm A. Oscillations of cyclic AMP in hormone-stimulated insulin-secreting beta-cells. Nature 439 (2006) 349-352
-
(2006)
Nature
, vol.439
, pp. 349-352
-
-
Dyachok, O.1
Isakov, Y.2
Sagetorp, J.3
Tengholm, A.4
-
9
-
-
27144448780
-
Mammalian cell-based optimization of the biarsenical-binding tetracysteine motif for improved fluorescence and affinity
-
Martin B.R., Giepmans B.N.G., Adams S.R., and Tsien R.Y. Mammalian cell-based optimization of the biarsenical-binding tetracysteine motif for improved fluorescence and affinity. Nat Biotechnol 23 (2005) 1308-1314
-
(2005)
Nat Biotechnol
, vol.23
, pp. 1308-1314
-
-
Martin, B.R.1
Giepmans, B.N.G.2
Adams, S.R.3
Tsien, R.Y.4
-
10
-
-
0037140742
-
New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications
-
Adams S.R., Campbell R.E., Gross L.A., Martin B.R., Walkup G.K., Yao Y., Llopis J., and Tsien R.Y. New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications. J Am Chem Soc 124 (2002) 6063-6076
-
(2002)
J Am Chem Soc
, vol.124
, pp. 6063-6076
-
-
Adams, S.R.1
Campbell, R.E.2
Gross, L.A.3
Martin, B.R.4
Walkup, G.K.5
Yao, Y.6
Llopis, J.7
Tsien, R.Y.8
-
11
-
-
33845196026
-
Golgi twins in late mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy
-
A double GFP TC tag labeled with ReAsH allows correlated light and electron microscopy to uncover the fate of Golgi vesicles during mitosis. The Golgi apparatus is disassembled into vesicles at the onset of mitosis, then forms into four clusters before reassembly.
-
Gaietta G.M., Giepmans B.N.G., Deerinck T.J., Smith W.B., Ngan L., Llopis J., Adams S.R., Tsien R.Y., and Ellisman M.H. Golgi twins in late mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy. Proc Natl Acad Sci 103 (2006) 17777-17782. A double GFP TC tag labeled with ReAsH allows correlated light and electron microscopy to uncover the fate of Golgi vesicles during mitosis. The Golgi apparatus is disassembled into vesicles at the onset of mitosis, then forms into four clusters before reassembly.
-
(2006)
Proc Natl Acad Sci
, vol.103
, pp. 17777-17782
-
-
Gaietta, G.M.1
Giepmans, B.N.G.2
Deerinck, T.J.3
Smith, W.B.4
Ngan, L.5
Llopis, J.6
Adams, S.R.7
Tsien, R.Y.8
Ellisman, M.H.9
-
12
-
-
0037028010
-
Transgenically encoded protein photoinactivation (FlAsH-FALI): acute inactivation of synaptotagmin I
-
Marek K.W., and Davis G.W. Transgenically encoded protein photoinactivation (FlAsH-FALI): acute inactivation of synaptotagmin I. Neuron 36 (2002) 805-813
-
(2002)
Neuron
, vol.36
, pp. 805-813
-
-
Marek, K.W.1
Davis, G.W.2
-
13
-
-
0037134035
-
Multicolor and electron microscopic imaging of connexin trafficking
-
Gaietta G., Deerinck T.J., Adams S.R., Bouwer J., Tour O., Laird D.W., Sosinsky G.E., Tsien R.Y., and Ellisman M.H. Multicolor and electron microscopic imaging of connexin trafficking. Science 296 (2002) 503-507
-
(2002)
Science
, vol.296
, pp. 503-507
-
-
Gaietta, G.1
Deerinck, T.J.2
Adams, S.R.3
Bouwer, J.4
Tour, O.5
Laird, D.W.6
Sosinsky, G.E.7
Tsien, R.Y.8
Ellisman, M.H.9
-
14
-
-
33748805519
-
Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins
-
Spagnuolo C.C., Vermeij R.J., and Jares-Erijman E.A. Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins. J Am Chem Soc 128 (2006) 12040-12041
-
(2006)
J Am Chem Soc
, vol.128
, pp. 12040-12041
-
-
Spagnuolo, C.C.1
Vermeij, R.J.2
Jares-Erijman, E.A.3
-
15
-
-
33747739472
-
Molecular basis of partial agonism at the neurotransmitter alpha2A-adrenergic receptor and Gi-protein heterotrimer
-
Nikolaev V.O., Hoffmann C., Bunemann M., Lohse M.J., and Vilardaga J.P. Molecular basis of partial agonism at the neurotransmitter alpha2A-adrenergic receptor and Gi-protein heterotrimer. J Biol Chem 281 (2006) 24506-24511
-
(2006)
J Biol Chem
, vol.281
, pp. 24506-24511
-
-
Nikolaev, V.O.1
Hoffmann, C.2
Bunemann, M.3
Lohse, M.J.4
Vilardaga, J.P.5
-
16
-
-
33646052569
-
FRET-based monitoring of conformational change of the beta2 adrenergic receptor in living cells
-
Nakanishi J., Takarada T., Yunoki S., Kikuchi Y., and Maeda M. FRET-based monitoring of conformational change of the beta2 adrenergic receptor in living cells. Biochem Biophys Res Commun 343 (2006) 1191-1196
-
(2006)
Biochem Biophys Res Commun
, vol.343
, pp. 1191-1196
-
-
Nakanishi, J.1
Takarada, T.2
Yunoki, S.3
Kikuchi, Y.4
Maeda, M.5
-
17
-
-
33646823839
-
Dynamics of the upper 50 kDa domain of myosin V examined with fluorescence resonance energy transfer
-
Sun M., Oakes J.L., Ananthanarayaman S., Hawley K.H., Tsien R., Adams S.R., and Yengo C.M. Dynamics of the upper 50 kDa domain of myosin V examined with fluorescence resonance energy transfer. J Biol Chem 281 (2006) 5711-5717
-
(2006)
J Biol Chem
, vol.281
, pp. 5711-5717
-
-
Sun, M.1
Oakes, J.L.2
Ananthanarayaman, S.3
Hawley, K.H.4
Tsien, R.5
Adams, S.R.6
Yengo, C.M.7
-
18
-
-
33646366159
-
Mutation of a conserved threonine in the third transmembrane helix of alpha- and beta-connexins creates a dominant-negative closed gap junction channel
-
Beahm D.L., Oshima A., Gaietta G.M., Hand G.M., Smock A.E., Zucker S.N., Toloue M.M., Chandrasekhar A., Nicholson B.J., and Sosinsky G.E. Mutation of a conserved threonine in the third transmembrane helix of alpha- and beta-connexins creates a dominant-negative closed gap junction channel. J Biol Chem 281 (2006) 7994-8009
-
(2006)
J Biol Chem
, vol.281
, pp. 7994-8009
-
-
Beahm, D.L.1
Oshima, A.2
Gaietta, G.M.3
Hand, G.M.4
Smock, A.E.5
Zucker, S.N.6
Toloue, M.M.7
Chandrasekhar, A.8
Nicholson, B.J.9
Sosinsky, G.E.10
-
20
-
-
0347004717
-
Monitoring protein stability and aggregation in vivo by real-time fluorescent labeling
-
Ignatova Z., and Gierasch L.M. Monitoring protein stability and aggregation in vivo by real-time fluorescent labeling. Proc Natl Acad Sci 101 (2004) 523-528
-
(2004)
Proc Natl Acad Sci
, vol.101
, pp. 523-528
-
-
Ignatova, Z.1
Gierasch, L.M.2
-
21
-
-
34247864013
-
Fluorescence imaging of amyloid formation in living cells by a functional, tetracysteine-tagged [alpha]-synuclein
-
Roberti M.J., Bertoncini C.W., Klement R., Jares-Erijman E.A., and Jovin T.M. Fluorescence imaging of amyloid formation in living cells by a functional, tetracysteine-tagged [alpha]-synuclein. Nat Meth 4 (2007) 345-351
-
(2007)
Nat Meth
, vol.4
, pp. 345-351
-
-
Roberti, M.J.1
Bertoncini, C.W.2
Klement, R.3
Jares-Erijman, E.A.4
Jovin, T.M.5
-
22
-
-
33745449038
-
Isolation of a high-affinity functional protein complex between OmcA and MtrC: two outer membrane decaheme c-type cytochromes of Shewanella oneidensis MR-1
-
Shi L., Chen B., Wang Z., Elias D.A., Mayer M.U., Gorby Y.A., Ni S., Lower B.H., Kennedy D.W., Wunschel D.S., et al. Isolation of a high-affinity functional protein complex between OmcA and MtrC: two outer membrane decaheme c-type cytochromes of Shewanella oneidensis MR-1. J Bacteriol 188 (2006) 4705-4714
-
(2006)
J Bacteriol
, vol.188
, pp. 4705-4714
-
-
Shi, L.1
Chen, B.2
Wang, Z.3
Elias, D.A.4
Mayer, M.U.5
Gorby, Y.A.6
Ni, S.7
Lower, B.H.8
Kennedy, D.W.9
Wunschel, D.S.10
-
23
-
-
33749005371
-
Quantitative four-dimensional tracking of cytoplasmic and nuclear HIV-1 complexes
-
FlAsH-labeled HIV particles were tracked through infection, passage through the cytoplasm, and, for the first time, viral particle dynamics could be measured inside the nucleus.
-
Arhel N., Genovesio A., Kim K.A., Miko S., Perret E., Olivo-Marin J.C., Shorte S., and Charneau P. Quantitative four-dimensional tracking of cytoplasmic and nuclear HIV-1 complexes. Nat Meth 3 (2006) 817-824. FlAsH-labeled HIV particles were tracked through infection, passage through the cytoplasm, and, for the first time, viral particle dynamics could be measured inside the nucleus.
-
(2006)
Nat Meth
, vol.3
, pp. 817-824
-
-
Arhel, N.1
Genovesio, A.2
Kim, K.A.3
Miko, S.4
Perret, E.5
Olivo-Marin, J.C.6
Shorte, S.7
Charneau, P.8
-
24
-
-
15344350796
-
Dynamic fluorescent imaging of human immunodeficiency virus type 1 gag in live cells by biarsenical labeling
-
Rudner L., Nydegger S., Coren L.V., Nagashima K., Thali M., and Ott D.E. Dynamic fluorescent imaging of human immunodeficiency virus type 1 gag in live cells by biarsenical labeling. J Virol 79 (2005) 4055-4065
-
(2005)
J Virol
, vol.79
, pp. 4055-4065
-
-
Rudner, L.1
Nydegger, S.2
Coren, L.V.3
Nagashima, K.4
Thali, M.5
Ott, D.E.6
-
25
-
-
33646410462
-
Identification of an intracellular trafficking and assembly pathway for HIV-1 gag
-
Perlman M., and Resh M.D. Identification of an intracellular trafficking and assembly pathway for HIV-1 gag. Traffic 7 (2006) 731-745
-
(2006)
Traffic
, vol.7
, pp. 731-745
-
-
Perlman, M.1
Resh, M.D.2
-
26
-
-
34250704796
-
Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator
-
d of 100 μM and a 10-fold fluorescence increase upon binding to calcium, this dye allowed the visualization of calcium waves in stimulated HeLa cells.
-
d of 100 μM and a 10-fold fluorescence increase upon binding to calcium, this dye allowed the visualization of calcium waves in stimulated HeLa cells.
-
(2007)
Nat Chem Biol
, vol.3
, pp. 423-431
-
-
Tour, O.1
Adams, S.R.2
Kerr, R.A.3
Meijer, R.M.4
Sejnowski, T.J.5
Tsien, R.W.6
Tsien, R.Y.7
-
27
-
-
31544453367
-
Selective labeling of extracellular proteins containing polyhistidine sequences by a fluorescein-nitrilotriacetic acid conjugate
-
Goldsmith C.R., Jaworski J., Sheng M., and Lippard S.J. Selective labeling of extracellular proteins containing polyhistidine sequences by a fluorescein-nitrilotriacetic acid conjugate. J Am Chem Soc 128 (2006) 418-419
-
(2006)
J Am Chem Soc
, vol.128
, pp. 418-419
-
-
Goldsmith, C.R.1
Jaworski, J.2
Sheng, M.3
Lippard, S.J.4
-
28
-
-
1842582037
-
Reversible site-selective labeling of membrane proteins in live cells
-
Guignet E.G., Hovius R., and Vogel H. Reversible site-selective labeling of membrane proteins in live cells. Nat Biotechnol 22 (2004) 440-444
-
(2004)
Nat Biotechnol
, vol.22
, pp. 440-444
-
-
Guignet, E.G.1
Hovius, R.2
Vogel, H.3
-
29
-
-
34250315787
-
Repetitive reversible labeling of proteins at polyhistidine sequences for single-molecule imaging in live cells
-
Guignet E.G., Segura J.-M., Hovius R., and Vogel H. Repetitive reversible labeling of proteins at polyhistidine sequences for single-molecule imaging in live cells. ChemPhysChem 8 (2007) 1221-1227
-
(2007)
ChemPhysChem
, vol.8
, pp. 1221-1227
-
-
Guignet, E.G.1
Segura, J.-M.2
Hovius, R.3
Vogel, H.4
-
30
-
-
33644516580
-
Specific and stable fluorescence labeling of histidine-tagged proteins for dissecting multi-protein complex formation
-
Lata S., Gavutis M., Tampe R., and Piehler J. Specific and stable fluorescence labeling of histidine-tagged proteins for dissecting multi-protein complex formation. J Am Chem Soc 128 (2006) 2365-2372
-
(2006)
J Am Chem Soc
, vol.128
, pp. 2365-2372
-
-
Lata, S.1
Gavutis, M.2
Tampe, R.3
Piehler, J.4
-
31
-
-
34247247126
-
2+-dye label reveals STIM1 surface exposure
-
2+-dye label reveals STIM1 surface exposure. Proc Natl Acad Sci 104 (2007) 3693-3697
-
(2007)
Proc Natl Acad Sci
, vol.104
, pp. 3693-3697
-
-
Hauser, C.T.1
Tsien, R.Y.2
-
32
-
-
33747613117
-
Oligo-Asp tag/Zn(II) complex probe as a new pair for labeling and fluorescence imaging of proteins
-
Ojida A., Honda K., Shinmi D., Kiyonaka S., Mori Y., and Hamachi I. Oligo-Asp tag/Zn(II) complex probe as a new pair for labeling and fluorescence imaging of proteins. J Am Chem Soc 128 (2006) 10452-10459
-
(2006)
J Am Chem Soc
, vol.128
, pp. 10452-10459
-
-
Ojida, A.1
Honda, K.2
Shinmi, D.3
Kiyonaka, S.4
Mori, Y.5
Hamachi, I.6
-
33
-
-
25144475961
-
Multicolor imaging of cell surface proteins
-
S. cerevisiae displaying an ACP fusion of cell wall protein Sag1p were labeled by sequential incubation with AcpS and CoA-Cy3, CoA-Cy5 or CoA-fluorescein. Older and newer protein generations could thus be distinguished by their different fluorescent labels. Tri-color images reveal that the secretion of Sag1p is polarized according to sites of cell wall construction, and the wall dividing mother from daughter cells is the last structure formed before separation.
-
Vivero-Pol L., George N., Krumm H., Johnsson K., and Johnsson N. Multicolor imaging of cell surface proteins. J Am Chem Soc 127 (2005) 12770-12771. S. cerevisiae displaying an ACP fusion of cell wall protein Sag1p were labeled by sequential incubation with AcpS and CoA-Cy3, CoA-Cy5 or CoA-fluorescein. Older and newer protein generations could thus be distinguished by their different fluorescent labels. Tri-color images reveal that the secretion of Sag1p is polarized according to sites of cell wall construction, and the wall dividing mother from daughter cells is the last structure formed before separation.
-
(2005)
J Am Chem Soc
, vol.127
, pp. 12770-12771
-
-
Vivero-Pol, L.1
George, N.2
Krumm, H.3
Johnsson, K.4
Johnsson, N.5
-
34
-
-
33745293709
-
Post-translational covalent labeling reveals heterogeneous mobility of individual G protein-coupled receptors in living cells
-
The neurokinin-1 receptor, tagged with ACP, bound the hormone NK-1 with the same kinetics as the untagged receptor. Labeling using AcpS allowed high-resolution localization of single receptor molecules, and tracking for up to 20 frames.
-
Prummer M., Meyer B.H., Franzini R., Segura J.M., George N., Johnsson K., and Vogel H. Post-translational covalent labeling reveals heterogeneous mobility of individual G protein-coupled receptors in living cells. ChemBioChem 7 (2006) 908-911. The neurokinin-1 receptor, tagged with ACP, bound the hormone NK-1 with the same kinetics as the untagged receptor. Labeling using AcpS allowed high-resolution localization of single receptor molecules, and tracking for up to 20 frames.
-
(2006)
ChemBioChem
, vol.7
, pp. 908-911
-
-
Prummer, M.1
Meyer, B.H.2
Franzini, R.3
Segura, J.M.4
George, N.5
Johnsson, K.6
Vogel, H.7
-
35
-
-
33344455206
-
Covalent labeling of cell-surface proteins for in-vivo FRET studies
-
Meyer B.H., Martinez K.L., Segura J.M., Pascoal P., Hovius R., George N., Johnsson K., and Vogel H. Covalent labeling of cell-surface proteins for in-vivo FRET studies. FEBS Lett 580 (2006) 1654-1658
-
(2006)
FEBS Lett
, vol.580
, pp. 1654-1658
-
-
Meyer, B.H.1
Martinez, K.L.2
Segura, J.M.3
Pascoal, P.4
Hovius, R.5
George, N.6
Johnsson, K.7
Vogel, H.8
-
36
-
-
33144473025
-
FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells
-
Meyer B.H., Segura J.M., Martinez K.L., Hovius R., George N., Johnsson K., and Vogel H. FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells. Proc Natl Acad Sci 103 (2006) 2138-2143
-
(2006)
Proc Natl Acad Sci
, vol.103
, pp. 2138-2143
-
-
Meyer, B.H.1
Segura, J.M.2
Martinez, K.L.3
Hovius, R.4
George, N.5
Johnsson, K.6
Vogel, H.7
-
37
-
-
19644391139
-
Targeting quantum dots to surface proteins in living cells with biotin ligase
-
Howarth M., Takao K., Hayashi Y., and Ting A.Y. Targeting quantum dots to surface proteins in living cells with biotin ligase. Proc Natl Acad Sci 102 (2005) 7583-7588
-
(2005)
Proc Natl Acad Sci
, vol.102
, pp. 7583-7588
-
-
Howarth, M.1
Takao, K.2
Hayashi, Y.3
Ting, A.Y.4
-
38
-
-
34248993985
-
Phage display evolution of a peptide substrate for yeast biotin ligase and application to two-color quantum dot labeling of cell surface proteins
-
Chen I., Choi Y.-A., and Ting A.Y. Phage display evolution of a peptide substrate for yeast biotin ligase and application to two-color quantum dot labeling of cell surface proteins. J Am Chem Soc 129 (2007) 6619-6625
-
(2007)
J Am Chem Soc
, vol.129
, pp. 6619-6625
-
-
Chen, I.1
Choi, Y.-A.2
Ting, A.Y.3
-
39
-
-
18744401100
-
Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase
-
Chen I., Howarth M., Lin W.Y., and Ting A.Y. Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase. Nat Meth 2 (2005) 99-104
-
(2005)
Nat Meth
, vol.2
, pp. 99-104
-
-
Chen, I.1
Howarth, M.2
Lin, W.Y.3
Ting, A.Y.4
-
40
-
-
33646038598
-
Transglutaminase-catalyzed site-specific conjugation of small-molecule probes to proteins in vitro and on the surface of living cells
-
Lin C.W., and Ting A.Y. Transglutaminase-catalyzed site-specific conjugation of small-molecule probes to proteins in vitro and on the surface of living cells. J Am Chem Soc 128 (2006) 4542-4543
-
(2006)
J Am Chem Soc
, vol.128
, pp. 4542-4543
-
-
Lin, C.W.1
Ting, A.Y.2
-
41
-
-
33846333918
-
Selective labeling of proteins by using protein farnesyltransferase
-
Duckworth B.P., Zhang Z., Hosokawa A., and Distefano M.D. Selective labeling of proteins by using protein farnesyltransferase. ChemBioChem 8 (2007) 98-105
-
(2007)
ChemBioChem
, vol.8
, pp. 98-105
-
-
Duckworth, B.P.1
Zhang, Z.2
Hosokawa, A.3
Distefano, M.D.4
-
42
-
-
34249076359
-
Introducing genetically encoded aldehydes into proteins
-
Carrico I.S., Carlson B.L., and Bertozzi C.R. Introducing genetically encoded aldehydes into proteins. Nat Chem Biol 3 (2007) 321-322
-
(2007)
Nat Chem Biol
, vol.3
, pp. 321-322
-
-
Carrico, I.S.1
Carlson, B.L.2
Bertozzi, C.R.3
-
43
-
-
33749243938
-
Visualizing odorant receptor trafficking in living cells down to the single-molecule level
-
The authors use AcpS and CoA-CypHer to transfer a pH sensitive dye to an ACP-tagged olfactory GPCR, providing direct evidence for constitutive receptor internalization, even in the absence of ligand.
-
Jacquier V., Prummer M., Segura J.M., Pick H., and Vogel H. Visualizing odorant receptor trafficking in living cells down to the single-molecule level. Proc Natl Acad Sci 103 (2006) 14325-14330. The authors use AcpS and CoA-CypHer to transfer a pH sensitive dye to an ACP-tagged olfactory GPCR, providing direct evidence for constitutive receptor internalization, even in the absence of ligand.
-
(2006)
Proc Natl Acad Sci
, vol.103
, pp. 14325-14330
-
-
Jacquier, V.1
Prummer, M.2
Segura, J.M.3
Pick, H.4
Vogel, H.5
-
44
-
-
27644508250
-
Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase
-
Yin J., Straight P.D., McLoughlin S.M., Zhou Z., Lin A.J., Golan D.E., Kelleher N.L., Kolter R., and Walsh C.T. Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase. Proc Natl Acad Sci 102 (2005) 15815-15820
-
(2005)
Proc Natl Acad Sci
, vol.102
, pp. 15815-15820
-
-
Yin, J.1
Straight, P.D.2
McLoughlin, S.M.3
Zhou, Z.4
Lin, A.J.5
Golan, D.E.6
Kelleher, N.L.7
Kolter, R.8
Walsh, C.T.9
-
45
-
-
34548860739
-
-
Zhou Z, Cironi P, Lin AJ, Xu Y, Hrvatin S, Golan DE, Silver PA, Walsh CT, Yin J: Genetically encoded short peptide tags for orthogonal protein labeling by Sfp and AcpS phosphopantetheinyl transferases. ACS Chem Biol 2007. Peptide substrates of AcpS and Sfp were selected from a phage-displayed library. AcpS and Sfp have orthogonal specificity, allowing cell-surface proteins TfR1 and EGFR to be labeled with Alexa 488 and Texas red, respectively.
-
-
-
-
46
-
-
33748776033
-
Synthesis and evaluation of bioorthogonal pantetheine analogues for in vivo protein modification
-
Meier J.L., Mercer A.C., Rivera Jr. H., and Burkart M.D. Synthesis and evaluation of bioorthogonal pantetheine analogues for in vivo protein modification. J Am Chem Soc 128 (2006) 12174-12184
-
(2006)
J Am Chem Soc
, vol.128
, pp. 12174-12184
-
-
Meier, J.L.1
Mercer, A.C.2
Rivera Jr., H.3
Burkart, M.D.4
-
48
-
-
22144460986
-
6-alkylguanine-DNA alkyltransferase for specific protein labeling in living cells
-
6-alkylguanine-DNA alkyltransferase for specific protein labeling in living cells. ChemBioChem 6 (2005) 1263-1269
-
(2005)
ChemBioChem
, vol.6
, pp. 1263-1269
-
-
Juillerat, A.1
Heinis, C.2
Sielaff, I.3
Barnikow, J.4
Jaccard, H.5
Kunz, B.6
Terskikh, A.7
Johnsson, K.8
-
49
-
-
33748525821
-
Fluorophores for live cell imaging of AGT fusion proteins across the visible spectrum
-
Keppler A., Arrivoli C., Sironi L., and Ellenberg J. Fluorophores for live cell imaging of AGT fusion proteins across the visible spectrum. Biotechniques 41 (2006) 167-174
-
(2006)
Biotechniques
, vol.41
, pp. 167-174
-
-
Keppler, A.1
Arrivoli, C.2
Sironi, L.3
Ellenberg, J.4
-
50
-
-
2342459815
-
Synthesis and characterization of bifunctional probes for the specific labeling of fusion proteins
-
Kindermann M., Sielaff I., and Johnsson K. Synthesis and characterization of bifunctional probes for the specific labeling of fusion proteins. Bioorg Med Chem Lett 14 (2004) 2725-2728
-
(2004)
Bioorg Med Chem Lett
, vol.14
, pp. 2725-2728
-
-
Kindermann, M.1
Sielaff, I.2
Johnsson, K.3
-
51
-
-
3042798464
-
Labeling of fusion proteins with synthetic fluorophores in live cells
-
Keppler A., Pick H., Arrivoli C., Vogel H., and Johnsson K. Labeling of fusion proteins with synthetic fluorophores in live cells. Proc Natl Acad Sci 101 (2004) 9955-9959
-
(2004)
Proc Natl Acad Sci
, vol.101
, pp. 9955-9959
-
-
Keppler, A.1
Pick, H.2
Arrivoli, C.3
Vogel, H.4
Johnsson, K.5
-
53
-
-
34447550874
-
Cellular trafficking of phospholambdan and formation of functional sarcoplasmic reticulum during myocyte differentiation
-
Stenoein D.L., Knyushko T.V., Londono M.P., Opresko K.L., Mayer M.U., Brady S.T., Squier T.C., and Bigelow D.J. Cellular trafficking of phospholambdan and formation of functional sarcoplasmic reticulum during myocyte differentiation. Am J Physiol Cell Physiol 292 (2007) C2084-C2094
-
(2007)
Am J Physiol Cell Physiol
, vol.292
-
-
Stenoein, D.L.1
Knyushko, T.V.2
Londono, M.P.3
Opresko, K.L.4
Mayer, M.U.5
Brady, S.T.6
Squier, T.C.7
Bigelow, D.J.8
-
54
-
-
33947274529
-
Propagation of centromeric chromatin requires exit from mitosis
-
Fluorescent and nonfluorescent substrates were applied in sequence to label AGT-tagged centromeric protein A at defined points in the cell cycle. Data from a series of such experiments resolve long-standing questions regarding stability, turnover, and the timing of centromeric deposition of this histone variant.
-
Jansen L.E.T., Black B.E., Foltz D.R., and Cleveland D.W. Propagation of centromeric chromatin requires exit from mitosis. J Cell Biol 176 (2007) 795-805. Fluorescent and nonfluorescent substrates were applied in sequence to label AGT-tagged centromeric protein A at defined points in the cell cycle. Data from a series of such experiments resolve long-standing questions regarding stability, turnover, and the timing of centromeric deposition of this histone variant.
-
(2007)
J Cell Biol
, vol.176
, pp. 795-805
-
-
Jansen, L.E.T.1
Black, B.E.2
Foltz, D.R.3
Cleveland, D.W.4
-
56
-
-
33748999738
-
Halotag interchangeable labeling technology for cell imaging and protein capture
-
Los G.V., Darzins A., Karassina N., Zimprich C., Learish R., McDougall M.G., Encell L.P., Friedman-Ohana R., Wood M., Vidurgiris G., et al. Halotag interchangeable labeling technology for cell imaging and protein capture. Promega Cell Notes 11 (2005) 2-6
-
(2005)
Promega Cell Notes
, vol.11
, pp. 2-6
-
-
Los, G.V.1
Darzins, A.2
Karassina, N.3
Zimprich, C.4
Learish, R.5
McDougall, M.G.6
Encell, L.P.7
Friedman-Ohana, R.8
Wood, M.9
Vidurgiris, G.10
-
57
-
-
33749036863
-
HaloTag (TM): a new versatile reporter gene system in plant cells
-
Lang C., Schulze J., Mendel R.R., and Hansch R. HaloTag (TM): a new versatile reporter gene system in plant cells. J Exp Bot 57 (2006) 2985-2992
-
(2006)
J Exp Bot
, vol.57
, pp. 2985-2992
-
-
Lang, C.1
Schulze, J.2
Mendel, R.R.3
Hansch, R.4
-
58
-
-
13444261101
-
Selective chemical labeling of proteins in living cells
-
Miller L.W., and Cornish V.W. Selective chemical labeling of proteins in living cells. Curr Opin Chem Biol 9 (2005) 56-61
-
(2005)
Curr Opin Chem Biol
, vol.9
, pp. 56-61
-
-
Miller, L.W.1
Cornish, V.W.2
-
59
-
-
34447292776
-
Inducing and sensing protein-protein interactions in living cells via selective crosslinking
-
Lemercier G., Gendreizig S., Kindermann, and Johnsson K. Inducing and sensing protein-protein interactions in living cells via selective crosslinking. Angew Chem Int Ed 46 (2007) 4281-4284
-
(2007)
Angew Chem Int Ed
, vol.46
, pp. 4281-4284
-
-
Lemercier, G.1
Gendreizig, S.2
Kindermann3
Johnsson, K.4
-
60
-
-
30444455027
-
Protein function microarrays based on self-immobilizing and self-labeling fusion proteins
-
Sielaff I., Arnold A., Godin G., Tugulu S., Klok H.-A., and Johnsson K. Protein function microarrays based on self-immobilizing and self-labeling fusion proteins. ChemBioChem 7 (2006) 194-202
-
(2006)
ChemBioChem
, vol.7
, pp. 194-202
-
-
Sielaff, I.1
Arnold, A.2
Godin, G.3
Tugulu, S.4
Klok, H.-A.5
Johnsson, K.6
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