Mammalian cell-based optimization of the biarsenical-binding tetracysteine motif for improved fluorescence and affinity

Nature Biotechnology

Volumn 23, Issue 10, 2005, Pages 1308-1314

  Martin, Brent R ^a   Giepmans, Ben N G ^a,b   Adams, Stephen R ^a   Tsien, Roger Y ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; CONCENTRATION (PROCESS); DYES; FLUORESCENCE; OPTIMIZATION; PROTEINS;

CELL SORTING; CELL-BASED OPTIMIZATION; DITHIOL; STAINING;

CELL CULTURE;

BETA ACTIN; CYSTEINE; DITHIOL DERIVATIVE; FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; ORGANOARSENIC DERIVATIVE; RETROVIRUS VECTOR;

AFFINITY LABELING; AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL STRAIN 3T3; CONTRAST ENHANCEMENT; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; FLUORESCENCE ANALYSIS; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; HUMAN CELL; MAMMAL CELL; PHOTOCHEMICAL QUENCHING; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN MOTIF; QUANTUM MECHANICS; SEQUENCE ANALYSIS; VIRUS RECOMBINATION;

ARSENICALS; BINDING SITES; CYSTEINE; FLUORESCENT DYES; HELA CELLS; HUMANS; MICROSCOPY, FLUORESCENCE; PROTEIN BINDING; PROTEIN ENGINEERING;

MAMMALIA;

EID: 27144448780     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt1136     Document Type: Article

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