Covalent labeling of cell-surface proteins for in-vivo FRET studies

FEBS Letters

Volumn 580, Issue 6, 2006, Pages 1654-1658

  Meyer, Bruno H ^a,b   Martinez, Karen L ^a,c   Segura, Jean Manuel ^a   Pascoal, Pedro ^a   Hovius, Ruud ^a   George, Nathalie ^a   Johnsson, Kai ^a   Vogel, Horst ^a  

^a EPFL   (Switzerland)

^b NOVARTIS PHARMA AG   (Switzerland)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

ACP labeling; Donor acceptor ratio; FRET microscopy; GPCR

Indexed keywords

ACYL CARRIER PROTEIN; CELL SURFACE PROTEIN; G PROTEIN COUPLED RECEPTOR; NEUROKININ 1 RECEPTOR;

ANALYTIC METHOD; ARTICLE; BINDING AFFINITY; CALCIUM SIGNALING; CONCENTRATION RESPONSE; CONTROLLED STUDY; COVALENT BOND; EMBRYO; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMAGE ANALYSIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN INTERACTION;

ACYL CARRIER PROTEIN; CARBOCYANINES; CELLS, CULTURED; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; HUMANS; MEMBRANE PROTEINS; PROTEIN INTERACTION MAPPING; RECEPTORS, G-PROTEIN-COUPLED; RECEPTORS, NEUROKININ-1; RECOMBINANT FUSION PROTEINS; SUBSTANCE P;

EID: 33344455206     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2006.02.007     Document Type: Article

References (27)

1. A. Wise, K. Gearing, and S. Rees Target validation of G protein-coupled receptors Drug Discov. Today 7 2002 235 246
2. G. Turcatti, K. Nemeth, M. Edgerton, U. Meseth, F. Talabot, M. Peitsch, J. Knowles, H. Vogel, and A. Chollet Probing the structure and function of the tachykinin NK2 receptor through biosynthetic incorporation of fluorescent amino acids at specific sites J. Biol. Chem. 271 1996 19991 19998
3. U. Gether, S. Lin, and B.K. Kobilka Fluorescent labeling of purified beta 2 adrenergic receptor. Evidence for ligand-specific conformational changes J. Biol. Chem. 270 1995 28268 28275
4. R.Y. Tsien The green fluorescent protein Annu. Rev. Biochem. 67 1998 509 544
5. R. Hovius, P. Vallotton, T. Wohland, and H. Vogel Fluorescence techniques. Shedding light on ligand-receptor interactions Trends Pharmacol. Sci. 21 2000 266 273
6. J.P. Vilardaga, M. Bunemann, C. Krasel, M. Castro, and M.J. Lohse Measurement of the millisecond activation switch of G protein-coupled receptors in living cells Nat. Biotechnol. 21 2003 807 812
7. M. Bunemann, M. Frank, and M.J. Lohse Gi protein activation in intact cells involves subunit rearrangement rather than dissociation Proc. Natl. Acad. Sci. USA 100 2003 16077 16082
8. M. Bouvier Oligomerization of G-protein-coupled transmitter receptors Nat. Rev. Neurosci. 2 2001 274 286
9. L. Stanasila, J.B. Perez, H. Vogel, and S. Cotecchia Oligomerization of the alpha 1a- and alpha 1b-adrenergic receptor subtypes. Potential implications in receptor internalization J. Biol. Chem. 278 2003 40239 40251
10. A. Miyawaki, and R.Y. Tsien Monitoring protein conformations and interactions by fluorescence resonance energy transfer between mutants of green fluorescent protein Methods Enzymol. 327 2000 472 500
11. J.Y. Vollmer, P. Alix, A. Chollet, K. Takeda, and J.L. Galzi Subcellular compartmentalization of activation and desensitization of responses mediated by NK2 neurokinin receptors J. Biol. Chem. 274 1999 37915 37922
12. T. Palanche, B. Ilien, S. Zoffmann, M.P. Reck, B. Bucher, S.J. Edelstein, and J.L. Galzi The neurokinin A receptor activates calcium and cAMP responses through distinct conformational states J. Biol. Chem. 276 2001 34853 34861
13. B.A. Griffin, S.R. Adams, and R.Y. Tsien Specific covalent labeling of recombinant protein molecules inside live cells Science 281 1998 269 272
14. A. Keppler, S. Gendreizig, T. Gronemeyer, H. Pick, H. Vogel, and K. Johnsson A general method for the covalent labeling of fusion proteins with small molecules in vivo Nat. Biotechnol. 21 2003 86 89
15. K.M. Marks, P.D. Braun, and G.P. Nolan A general approach for chemical labeling and rapid, spatially controlled protein inactivation Proc. Natl. Acad. Sci. USA 101 2004 9982 9987
16. J. Yin, F. Liu, X. Li, and C.T. Walsh Labeling proteins with small molecules by site-specific Posttranslational modification J. Am. Chem. Soc. 126 2004 7754 7755
17. E.G. Guignet, R. Hovius, and H. Vogel Reversible site-selective labeling of membrane proteins in live cells Nat. Biotechnol. 22 2004 440 444
18. N. George, H. Pick, H. Vogel, N. Johnsson, and K. Johnsson Specific labeling of cell surface proteins with chemically diverse compounds J. Am. Chem. Soc. 126 2004 8896 8897
19. I. Chen, M. Howarth, W.Y. Lin, and A.Y. Ting Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase Nat. Meth. 2 2005 99 104
20. G.W. Gordon, G. Berry, X.H. Liang, B. Levine, and B. Herman Quantitative fluorescence resonance energy transfer measurements using fluorescence microscopy Biophys. J. 74 1998 2702 2713
21. M.G. Erickson, B.A. Alseikhan, B.Z. Peterson, and D.T. Yue Preassociation of calmodulin with voltage-gated Ca(2+) channels revealed by FRET in single living cells Neuron 31 2001 973 985
22. O. Dery, K.A. Defea, and N.W. Bunnett Protein kinase C-mediated desensitization of the neurokinin 1 receptor Am. J. Physiol. Cell Physiol. 280 2001 C1097 C1106
23. E.F. Grady, A.M. Garland, P.D. Gamp, M. Lovett, D.G. Payan, and N.W. Bunnett Delineation of the endocytic pathway of substance P and its seven-transmembrane domain NK1 receptor Mol. Biol. Cell 6 1995 509 524
24. M.A. Ayoub, C. Couturier, E. Lucas-Meunier, S. Angers, P. Fossier, M. Bouvier, and R. Jockers Monitoring of ligand-independent dimerization and ligand-induced conformational changes of melatonin receptors in living cells by bioluminescence resonance energy transfer J. Biol. Chem. 277 2002 21522 21528
25. N.C. Shaner, R.E. Campbell, P.A. Steinbach, B.N.G. Giepmans, A.E. Palmer, and R.Y. Tsien Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein Nat. Biotechnol. 22 2004 1567 1572
26. D.S. Dahan, M.I. Dibas, E.J. Petersson, V.C. Auyeung, B. Chanda, F. Bezanilla, D.A Dougherty, and H.A Lester A fluorophore attached to nicotinic acetylcholine receptor beta M2 detects productive binding of agonist to the alpha delta site Proc. Natl. Acad. Sci. USA 101 2004 10195 10200
27. E. Ilegems, H. Pick, C. Deluz, S. Kellenberger, and H. Vogel Ligand binding transmits conformational changes across the membrane-spanning region to the intracellular side of the 5-HT(3) serotonin receptor Chembiochem 6 2005 2180 2185