Directed evolution of O6-alkylguanine-DNA alkyltransferase for applications in protein labeling

Protein Engineering, Design and Selection

Volumn 19, Issue 7, 2006, Pages 309-316

  Gronemeyer, Thomas ^a   Chidley, Christopher ^a   Juillerat, Alexandre ^a   Heinis, Christian ^a   Johnsson, Kai ^a  

^a EPFL   (Switzerland)

Author keywords

Directed evolution; O6 alkylguanine DNA alkyltransferases; Phage display; Protein labeling

Indexed keywords

BACTERIOPHAGES; CATALYST SELECTIVITY; DNA; OXYGEN; PROTEINS; STABILITY;

DIRECTED EVOLUTION; O6-ALKYLGUANINE-DNA ALKYLTRANSFERASES; PHAGE DISPLAY; PROTEIN LABELING;

ENZYME KINETICS;

6 O ALKYLGUANINE DNA ALKYLTRANSFERASE; 6 O BENZYLGUANINE; 6 O BENZYLGUANINE DERIVATIVE; HYBRID PROTEIN; MUTANT PROTEIN; UNCLASSIFIED DRUG; DRUG DERIVATIVE; GUANINE; METHYLATED DNA PROTEIN CYSTEINE METHYLTRANSFERASE; O(6) BENZYLGUANINE; O(6)-BENZYLGUANINE; PEPTIDE LIBRARY;

ARTICLE; CHEMICAL LABELING; CONTROLLED STUDY; DIRECTED MOLECULAR EVOLUTION; EMBRYO; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME STABILITY; HUMAN; HUMAN CELL; IN VITRO STUDY; MAMMAL CELL; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN LABELING; BINDING SITE; CELL CULTURE; CELL MEMBRANE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR EVOLUTION; MOLECULAR WEIGHT; SITE DIRECTED MUTAGENESIS; STAINING;

MAMMALIA;

BINDING SITES; CELL MEMBRANE; CELLS, CULTURED; ESCHERICHIA COLI; EVOLUTION, MOLECULAR; GUANINE; MODELS, MOLECULAR; MOLECULAR WEIGHT; MUTAGENESIS, SITE-DIRECTED; O(6)-METHYLGUANINE-DNA METHYLTRANSFERASE; PEPTIDE LIBRARY; RECOMBINANT FUSION PROTEINS; STAINING AND LABELING;

EID: 33745712857     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzl014     Document Type: Article

References (22)

1. Baker,K., Bleczinski,C., Lin,H., Salazar-Jimenez,G., Sengupta,D., Krane,S. and Cornish,V.W. (2002) Proc Natl Acad. Sci. USA, 99, 16537-16542.
2. Carter,B.T., Lin,H., Goldberg,S.D., Althoff,E.A., Raushel,J. and Cornish,V.W. (2005) Chembiochem, 6, 2055-2067.
3. Chen,R., Knez,J.J., Merrick,W.C. and Medof,M.E. (2001) J. Cell Biochem., 84, 68-83.
4. Daniels,D.S., Woo,T.T., Luu,K.X., Noll,D.M., Clarke,N.D., Pegg,A.E. and Tainer,J.A. (2004) Nat. Struct. Mol. Biol., 11, 714-720.
5. Davis,B.M., Encell,L.P., Zielske,S.P., Christians,F.C., Liu,L., Friebert,S.E., Loeb,L.A. and Gerson,S.L. (2001) Proc. Natl Acad. Sci. USA, 98, 4950-4954.
6. Encell,L.P., Coates,M.M. and Loeb,L.A. (1998) Cancer Res., 58, 1013-1020.
7. Firestine,S.M., Salinas,F., Nixon,A.E., Baker,S.J. and Benkovic,S.J. (2000) Nat. Biotechnol, 18, 544-547.
8. Gendreizig,S., Kindermann,M. and Johnsson,K (2003) J. Am. Chem. Soc., 125, 14970-14971.
9. George,N., Pick,H., Vogel,H., Johnsson,N. and Johnsson,K. (2004) J. Am. Chem. Soc., 126, 8896-8897.
10. Gietz,R.D. and Woods,R.A. (2002) Methods Enzymol., 350, 87-96.
11. Hiscox,S., Hallett,M.B., Morgan,B.P. and van den Berg,C.W. (2002) Biochem. Biophys. Res. Commun., 293, 714-721.
12. Johnsson,N. and Johnsson,K. (2003) Chembiochem, 4, 803-810.
13. Juillerat,A., Gronemeyer,T., Keppler,A., Gendreizig,S., Pick,H., Vogel,H. and Johnsson,K. (2003) Chem. Biol., 10, 313-317.
14. Juillerat,A., Heinis,C., Sielaff,I., Barnikow,J., Jaccard,H., Kunz,B., Terskikh,A. and Johnsson,K. (2005) Chembiochem, 6, 1263-1269.
15. Keppler,A., Gendreizig,S., Gronemeyer,T., Pick,H., Vogel,H. and Johnsson,K. (2003) Nat. Biotechnol., 21, 86-89.
16. Keppler,A., Kindermann,M., Gendreizig,S., Pick,H., Vogel,H. and Johnsson,K. (2004a) Methods, 32, 437-444.
17. Keppler,A., Pick,H., Arrivoli,C., Vogel,H. and Johnsson,K. (2004b) Proc. Natl Acad. Sci. USA, 101, 9955-9959.
18. Krebber,A., Bornhauser,S., Burmester,J., Honegger,A., Willuda,J., Bosshard,H.R. and Pluckthun,A. (1997) J. Immunol. Methods, 201, 35-55.
19. Kuchner,O. and Arnold,F.H. (1997) Trends Biotechnol., 15, 523-530.
20. Lin,H., Tao,H. and Cornish,V.W. (2004) J. Am. Chem. Soc., 126, 15051-15059.
21. Pegg,A.E. (2000) Mutat Res., 462, 83-100.
22. Zaccolo,M. and Gherardi,E. (1999) J. Mol. Biol., 285, 775-783.