Engineering substrate specificity of O6-alkylguanine-DNA alkyltransferase for specific protein labeling in living cells

ChemBioChem

Volumn 6, Issue 7, 2005, Pages 1263-1269

  Juillerat, Alexandre ^a   Heinis, Christian ^a   Sielaff, India ^a   Barnikow, Jan ^a,b   Jaccard, Hugues ^a   Kunz, Beatrice ^a   Terskikh, Alexey ^a   Johnsson, Kai ^a  

^a EPFL   (Switzerland)

^b Institute for Biological Engineering and Biotechnology ^*  (Switzerland)

Author keywords

Alkyltransferases; Directed evolution; Fluorescent probes; Protein engineering; Protein modifications

Indexed keywords

(4 BROMOTHIOPHEN 2 YL)METHANOL; 6 O ALKYLGUANINE DNA ALKYLTRANSFERASE; 6 O ALKYLGUANINE DNA ALKYLTRANSFERASE INHIBITOR; HYBRID PROTEIN; N 9 CYCLOPENTYL 6 CHLOROGUANINE; N 9 CYCLOPENTYL O 6 (4BROMOTHENYL)GUANINE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CHEMICAL LABELING; CHEMICAL STRUCTURE; CONTROLLED STUDY; ENZYME INACTIVATION; ENZYME SPECIFICITY; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; SYNTHESIS;

ANIMALS; CHO CELLS; CRICETINAE; FLUORESCENT DYES; HUMANS; MODELS, MOLECULAR; O(6)-METHYLGUANINE-DNA METHYLTRANSFERASE; PROTEIN ENGINEERING; PROTEINS; RECOMBINANT FUSION PROTEINS; SUBSTRATE SPECIFICITY; TRANSFECTION;

MAMMALIA;

EID: 22144460986     PISSN: 14394227     EISSN: None     Source Type: Journal    
DOI: 10.1002/cbic.200400431     Document Type: Article

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