Fusion of a recombinant antibody fragment with a homo-amino-acid polymer: Effects on biophysical properties and prolonged plasma half-life

Protein Engineering, Design and Selection

Volumn 20, Issue 6, 2007, Pages 273-284

  Schlapschy, Martin ^a   Theobald, Ina ^a   Mack, Hildegard ^a   Schottelius, Margret ^b   Wester, Hans Jürgen ^b   Skerra, Arne ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Biopharmaceutical; Hydrodynamic volume; Kidney filtration; Random coil; Therapeutic protein

Indexed keywords

AMINO ACIDS; ESCHERICHIA COLI; FUSION REACTIONS; GENETIC ENGINEERING; HOMOPOLYMERIZATION; POLYETHYLENE GLYCOLS;

BIOPHARMACEUTICAL; HYDRODYNAMIC VOLUME; KIDNEY FILTRATION; RANDOM COIL;

ANTIBODIES;

AMINO ACID; EPIDERMAL GROWTH FACTOR RECEPTOR 2; HYBRID PROTEIN; IMMUNOGLOBULIN F(AB) FRAGMENT; MACROGOL; POLYMER; POLYPEPTIDE; RECOMBINANT ANTIBODY; TRASTUZUMAB;

AFFINITY CHROMATOGRAPHY; ANIMAL EXPERIMENT; ANTIGEN BINDING; ARTICLE; CONTROLLED STUDY; DRUG HALF LIFE; ESCHERICHIA COLI; FEMALE; GEL FILTRATION; GENETIC ENGINEERING; HYDRODYNAMICS; MOUSE; NONHUMAN; PLASMA HALF LIFE; PRIORITY JOURNAL; RADIOIODINATION;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; BASE SEQUENCE; CIRCULAR DICHROISM; FEMALE; GLYCINE; HALF-LIFE; IMMUNOGLOBULIN FAB FRAGMENTS; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; PLASMA; POLYMERS; RECEPTOR, ERBB-2; RECOMBINANT FUSION PROTEINS; SERINE;

ESCHERICHIA COLI; MUS;

EID: 34547735974     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzm020     Document Type: Article

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