An engineered IN-1 Fab fragment with improved affinity for the nogo-A axonal growth inhibitor permits immunochemical detection and shows enhanced neutralizing activity

Protein Engineering

Volumn 15, Issue 11, 2002, Pages 931-941

  Fiedler, M ^a   Horn, C ^a,b   Bandtlow, C ^c   Schwab, M E ^d   Skerral, A ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b GOETHE UNIVERSITY   (Germany)

^c UNIVERSITY OF INNSBRUCK   (Austria)

^d ETH ZURICH   (Switzerland)

Author keywords

Affinity maturation; Axonal regeneration; Bacterial expression; Neuronal inhibitor; Random mutagenesis

Indexed keywords

CELL ANTIGEN; IMMUNOGLOBULIN M ANTIBODY; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY IN 1; MYELIN; PROTEIN INHIBITOR; PROTEIN NOGO A; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CELL CULTURE; CENTRAL NERVOUS SYSTEM; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOPLASM; DISSOCIATION; ESCHERICHIA COLI; EXON; GENE TARGETING; GENETIC SCREENING; HISTOCHEMISTRY; IMMUNOCHEMISTRY; MEASUREMENT; NERVE CELL PLASTICITY; NERVE FIBER GROWTH; NERVE FIBER REGENERATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN FUNCTION; PROTEIN SECRETION; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; SURFACE PLASMON RESONANCE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MURINAE;

EID: 0036878139     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/15.11.931     Document Type: Article

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