tRNA-assisted overproduction of eukaryotic ribosomal proteins

Protein Expression and Purification

Volumn 18, Issue 3, 2000, Pages 346-354

  Dieci, Giorgio ^a   Bottarelli, Lorena ^a   Ballabeni, Andrea ^a   Ottonello, Simone ^a  

^a UNIVERSITY OF PARMA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; BACTERIAL PROTEIN; CODON; CYTOPLASM; EXPRESSION VECTOR; GENE OVEREXPRESSION; PLASMID VECTOR; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; RIBOSOME; RIBOSOME PROTEIN; TRANSFER RNA;

ARABIDOPSIS THALIANA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; HOMO SAPIENS; SACCHAROMYCES CEREVISIAE;

EID: 0034007186     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1203     Document Type: Article

References (47)

1. Frank J., Zhu J., Penczek P., Li Y., Srivastava S., Verschoor A., Radermacher M., Grassucci R., Lata R. K., Agrawal R. K. A model of protein synthesis based on cryo-electron microscopy of the E. coli ribosome. Nature. 376:1995;441-444.
2. Stark H., Mueller F., Orlova E. V., Schatz M., Dube P., Erdemir T., Zemlin F., Brimacombe R., van Heel M. The 70S Escherichia coli ribosome at 23 Å resolution: Fitting the ribosomal RNA. Structure. 3:1995;815-821.
3. Ban N., Freeborn B., Nissen P., Penczek P., Grassucci R. A., Sweet R., Frank J., Moore P. B., Steitz T. A. A 9 A resolution X-ray crystallographic map of the large ribosomal subunit. Cell. 93:1998;1105-1115.
4. Moore P. B. The three-dimensional structure of the ribosome and its components. Annu. Rev. Biophys. Biomol. Struct. 27:1998;35-58.
5. Ramakrishnan V., White S. W. Ribosomal protein structures: Insights into the architecture, machinery and evolution of the ribosome. Trends Biochem. Sci. 23:1998;208-212.
6. Stoldt M., Wohnert J., Gorlach M., Brown L. R. The NMR structure of Escherichia coli ribosomal protein L25 shows homology to general stress proteins and glutaminyl-tRNA synthetases. EMBO J. 17:1998;6377-6384.
7. Nakagawa A., Nakashima T., Taniguchi M., Hosaka H., Kimura M., Tanaka I. The three-dimensional structure of the RNA-binding domain of ribosomal protein L2, a protein at the peptidyl transferase center of the ribosome. EMBO J. 18:1999;1459-1467.
8. Clemons W. M. Jr., May J. L. C., Wimberly B. T., McCutcheon J. P., Capel M. S., Ramakrishnan V. Structure of a bacterial 30S ribosomal subunit at 5.5 Å resolution. Nature. 400:1999;833-840.
9. Ban N., Nissen P., Hansen J., Capel M., Moore P. B., Steitz T. A. Placement of proteins and RNA structures into a 5Å-resolution map of the 50S ribosomal subunit. Nature. 400:1999;841-847.
10. Goffeau A., Barrel B. G., Bussey H., Davis R. W., Dujon B., Feldmann H., Galibert G., Hoheisel J. D., Jacq C., Johnston M., Louis E. J., Mewes H. W., Murakami Y., Philippsen P., Tettelin H., Oliver S. G. Life with 6000 genes. Science. 274:1996;546-567.
11. Hodgkin J., Horvitz H. R., Jasny B. R., Kimble J. C. elegans: Sequence to biology. Science. 282:1998;2011.
12. Planta R. J., Mager W. H. The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae. Yeast. 14:1998;471-477.
13. Wool I. G., Chan Y.-L., Glück A. Structure and evolution of mammalian ribosomal proteins. Biochem. Cell. Biol. 73:1995;933-947.
14. Verschoor A., Srivastava S., Grassucci R., Frank J. Native 3D structure of eukaryotic 80S ribosome: Morphological homology with E. coli 70S ribosome. J. Cell. Biol. 133:1996;495-505.
15. Verschoor A., Warner J. R., Srivastava S., Grassucci R. A., Frank J. Three-dimensional structure of the yeast ribosome. Nucleic Acids Res. 26:1998;655-661.
16. Srivastava S., Verschoor A., Radermacher M., Grassucci R., Frank J. Three-dimensional reconstruction of mammalian 40 S ribosomal subunit embedded in ice. J. Mol. Biol. 245:1995;461-466.
17. Raué H. A., Mager W. H., Planta R. J. Structural and functional analysis of yeast ribosomal proteins. Methods Enzymol. 194:1991;453-477.
18. Threadgill G. J., Conrad R. C., Cannon M., Craven G. R. A rapid and preparative method for the separation of yeast ribosomal proteins by using high-performance liquid chromatography. Biochem. J. 244:1987;523-532.
19. Minet M., Dufour M. E., Lacroute F. Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs. Plant J. 2:1992;417-422.
20. Studier F. W., Rosenberg A. H., Dunn J. J., Dubendorff J. W. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:1990;60-89.
21. Brinkmann U., Mattes R. E., Buckel P. High-level expression of recombinant genes in Escherichia coli is dependent on the availability of the dnaY gene product. Gene. 85:1989;109-114.
22. Adams S. M., Helps N. R., Sharp M. G., Brammar W. J., Walker R. A., Varley J. M. Isolation and characterization of a novel gene with differential expression in benign and malignant human breast tumour. Hum. Mol. Genet. 1:1992;91-96.
23. Hemmerich P., von Mikecz A., Neumann F., Soezeri O., Wolff-Vorbeck G., Zoebelein R., Krawinkel U. Structural and functional properties of ribosomal protein L7 from humans and rodent. Nucleic Acids Res. 21:1993;223-231.
24. von Mikecz A. H., Hemmerich P. H., Peter H.-H., Krawinkel U. Autoantigenic epitopes on eukaryotic L7. Clin. Exp. Immunol. 100:1995;205-213.
25. Chen G. T., Inouye M. Role of the AGA/AGG codons, the rarest codons in global gene expression in Escherichia coli. Genes Dev. 8:1994;2641-2652.
26. Stempfer G., Hoell-Neugebauer B., Rudolph R. Improved refolding of an immobilized fusion protein. Nat. Biotechnol. 14:1996;329-333.
27. Draper D. E., Reynaldo L. P. RNA binding strategies of ribosomal proteins. Nucleic Acids Res. 27:1999;381-388.
28. Todokoro K., Ulbrich N., Chan Y. L., Wool I. G. Characterization of the binding of rat liver ribosomal proteins L6, L8, L19, S9, and S13 to 5.8 S ribosomal ribonucleic acid. J. Biol. Chem. 256:1981;7207-7212.
29. Lee J. C., Henry B., Yeh Y.-C. Binding of proteins from the large ribosomal subunits to 5.8 S rRNA of Saccharomyces cerevisiae. J. Biol. Chem. 258:1983;854-858.
30. Lee-Chuan C. Y., Lee J. C. Yeast ribosomal proteins L4, L17, L20 and L25 exhibit different binding characteristics for the yeast 35S precursor rRNA. Biochim. Biophys. Acta. 1442:1998;139-148.
31. Neumann F., Hemmerich P., von Mikecz A., Peter H.-H., Krawinkel U. Human ribosomal protein L7 inhibits cell-free translation in reticulocyte lysates and affects the expression of nuclear proteins upon stable transfection into Jurkat T-lymphoma cells. Nucleic Acids Res. 23:1995;195-202.
32. Fewell S. W., Woolford J. L. Jr. Ribosomal protein S14 of Saccharomyces cerevisiae regulates its expression by binding to RPS14B pre-mRNA and to 18S rRNA. Mol. Cell. Biol. 19:1999;826-834.
33. Schenk P. M., Baumann S., Mattes R., Steinbiss H.-H. Improved high-level expression system for eukaryotic genes in Escherichia coli using T7 RNA polymerase and rare ArgtRNAs. Biotechniques. 19:1995;196-200.
34. Jeong W., Shin H. C. Supply of the argU gene product allows high-level expression of recombinant human interferon-alpha 2a in Escherichia coli. Biotechnol. Lett. 20:1998;19-22.
35. Hill A. M., Cane D. E., Mau C. J. D., West C. A. High level expression of Ricinus communis casbene synthase in Escherichia coli and characterization of the recombinant enzyme. Arch. Biochem. Biophys. 336:1996;283-289.
36. Seidel H. M., Pompliano D. L., Knowles J. R. Phosphonate biosynthesis: Molecular cloning of the gene for phosphoenolpyruvate mutase from Tetrahymena pyriformis and overexpression of the gene product in Escherichia coli. Biochemistry. 31:1992;2598-2608.
37. Kim R., Sandler S. J., Goldman S., Yokota H., Clark A. J., Kim S. H. Overexpression of archaeal proteins in Escherichia coli. Biotechnol. Lett. 20:1998;207-210.
38. Kane J. F. Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli. Curr. Opin. Biotechnol. 6:1995;494-500.
39. Varenne S., Lazdunski C. Effect of distribution of unfavourable codons on the maximum rate of gene expression by a heterologous organism. J. Theor. Biol. 120:1986;99-110.
40. Ottonello S., Ballabeni A., Soncini C., Dieci G. High level expression in E. coli and purification of yeast transcription factor IIIA. Biochem. Biophys. Res. Commun. 203:1994;1217-1223.
41. Librizzi M. D., Moir R. D., Brenowitz M., Willis I. M. Expression and purification of the RNA polymerase III transcription specificity factor IIIB70 from Saccharomyces cerevisiae and its cooperative binding with TATA-binding protein. J. Biol. Chem. 271:1996;32695-32701.
42. Lilie H., Schwarz E., Rudolph R. Advances in refolding of proteins produced in E. coli. Curr. Opin. Biotechnol. 9:1998;497-502.
43. Hemmerich P., Bosbach S., von Mikecz A., Krawinkel U. Human ribosomal protein L7 binds RNA with an α-helical arginine-rich and lysine-rich domain. Eur. J. Biochem. 245:1997;549-556.
44. Fried H. M., Warner J. R. Cloning of yeast gene for trichodermin resistance and ribosomal protein L3. Proc. Natl. Acad. Sci. USA. 78:1981;238-242.
45. Larkin J. C., Woolford J. L. Jr. Molecular cloning and analysis of the CRY1 gene: A yeast ribosomal protein gene. Nucleic Acids Res. 11:1983;403-420.
46. Kawai S., Murao S., Mochizuki M., Shibuya I., Yano K., Takagi M. Drastic alteration of cycloheximide sensitivity by substitution of one amino acid in the L41 ribosomal protein of yeasts. J. Bacteriol. 174:1992;254-262.
47. Gomez-Lorenzo M. G., Garcia-Bustos J. F. Ribosomal P-protein stalk function is targeted by sordarin antifungals. J. Biol. Chem. 273:1998;25041-25044.