Biocatalytic conversion of avermectin to 4″-oxo-avermectin: Improvement of cytochrome P450 monooxygenase specificity by directed evolution

Applied and Environmental Microbiology

Volumn 73, Issue 13, 2007, Pages 4317-4325

  Trefzer, Axel ^a,d   Jungmann, Volker ^b   Molnár, István ^c,e   Botejue, Ajit ^a   Buckel, Dagmar ^b,f   Frey, Gerhard ^a   Hill, D Steven ^c,g   Jörg, Mario ^b   Ligon, James M ^c,g   Mason, Dylan ^a,f   Moore, David ^a,f   Pachlatko, J Paul ^b,f   Richardson, Toby H ^a,h   Spangenberg, Petra ^b,f   Wall, Mark A ^a   Zirkle, Ross ^c,i   Stege, Justin T ^a  

^a DIVERSA CORPORATION   (United States)

^b SYNGENTA CROP PROTECTION AG   (Switzerland)

^c SYNGENTA BIOTECHNOLOGY INC   (United States)

^d DSM RESOLVE   (Netherlands)

^e UNIVERSITY OF ARIZONA   (United States)

^f BASF Agricultural Products ^*  (Germany)

^g BASF Agricultural Product Group   (United States)

^h Synthetic Genomics Inc   (United States)

ⁱ Martek Biosciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCATALYTIC REACTION SYSTEM; INDUSTRIAL PROCESSES; MOLECULAR EVOLUTION;

BIOCATALYSTS; CATALYST ACTIVITY; INSECTICIDES; PROTEINS; SYNTHESIS (CHEMICAL);

MICROBIOLOGY;

4'' OXO AVERMECTIN; AVERMECTIN; CYTOCHROME P450; CYTOCHROME P450 107Z; EMAMECTIN; ENZYME VARIANT; INSECTICIDE; ISOENZYME; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE;

CATALYSIS; CHEMICAL REACTION; CYTOCHROME; DRUG; GENE; INSECTICIDE; MUTATION; PROTEIN;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BIOCATALYST; BIOSYNTHESIS; BIOTRANSFORMATION; CATALYST SUPPORT; COMMERCIAL PHENOMENA; CONTROLLED STUDY; CYP107Z GENE; ENZYME SPECIFICITY; GENE INSERTION; GENEREASSEMBLY; GENETIC RECOMBINATION; HIGH THROUGHPUT SCREENING; MOLECULAR EVOLUTION; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; TECHNIQUE;

BIOTRANSFORMATION; CYTOCHROME P-450 ENZYME SYSTEM; DIRECTED MOLECULAR EVOLUTION; DISACCHARIDES; GENE LIBRARY; GENES, BACTERIAL; INSECTICIDES; IVERMECTIN; MOLECULAR SEQUENCE DATA; MUTATION; OXIDATION-REDUCTION; STREPTOMYCES; SUBSTRATE SPECIFICITY;

EID: 34447519310     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.02676-06     Document Type: Article

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