Biocatalytic conversion of avermectin to 4″-oxo-avermectin: Heterologous expression of the ema1 cytochrome p450 monooxygenase

Applied and Environmental Microbiology

Volumn 71, Issue 11, 2005, Pages 6977-6985

  Molnár, István ^a,c   Hill, D Steven ^a,d   Zirkle, Ross ^a,e   Hammer, Philip E ^a,f   Gross, Frank ^a,g   Buckel, Thomas G ^b,h   Jungmann, Volker ^b   Pachlatko, Johannes Paul ^b,i   Ligon, James M ^a,d  

^a SYNGENTA BIOTECHNOLOGY INC   (United States)

^b SYNGENTA CROP PROTECTION AG   (Switzerland)

^c UNIVERSITY OF ARIZONA   (United States)

^d BASF Agricultural Product Group   (United States)

^e Martek Biosciences   (United States)

^f Athenix Corp   (United States)

^g Gene Bridges GmbH   (Germany)

^h F HOFFMANN LA ROCHE LTD   (Switzerland)

ⁱ NONE   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; INSECTICIDES; OPTIMIZATION; OXIDATION; SOLVENTS;

AVERMECTIN; BIOCATALYTIC CONVERSION; TTA CODON;

BIOCATALYSTS;

4'' OXOAVERMECTIN; AVERMECTIN; AVERMECTIN DERIVATIVE; BACTERIAL PROTEIN; CYTOCHROME P450; EMAMECTIN BENZOATE; FERREDOXIN; FERREDOXIN REDUCTASE; INSECTICIDE; LEUCINE; OXIDOREDUCTASE; PROTEIN EMA1; SOLVENT; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE;

GENE EXPRESSION;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BIOCATALYST; CODON; ELECTRON TRANSPORT; EMA1 GENE; ENZYME MECHANISM; GENE EXPRESSION; GENE VECTOR; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION; PROMOTER REGION; PSEUDOMONAS PUTIDA; STEREOCHEMISTRY; STREPTOMYCES; STREPTOMYCES AVERMITILIS; STREPTOMYCES COELICOLOR; STREPTOMYCES LIVIDANS; STREPTOMYCES TUBERCIDICUS;

AMINO ACID SEQUENCE; CYTOCHROME P-450 ENZYME SYSTEM; DISACCHARIDES; FERREDOXINS; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; INDUSTRIAL MICROBIOLOGY; IVERMECTIN; OXIDATION-REDUCTION; OXIDOREDUCTASES; PROMOTER REGIONS (GENETICS); PSEUDOMONAS PUTIDA; SEQUENCE ALIGNMENT; STREPTOMYCES; TRANSFORMATION, BACTERIAL;

BACTERIA (MICROORGANISMS); PSEUDOMONAS PUTIDA; STREPTOMYCES; STREPTOMYCES AVERMITILIS; STREPTOMYCES COELICOLOR; STREPTOMYCES LIVIDANS; STREPTOMYCES TUBERCIDICUS;

EID: 32044440286     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.11.6977-6985.2005     Document Type: Article

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