Colorimetric high-throughput assay for alkene epoxidation catalyzed by cytochrome P450 BM-3 variant 139-3

Journal of Biomolecular Screening

Volumn 9, Issue 2, 2004, Pages 141-146

  Alcalde, Miguel ^a   Farinas, Edgardo T ^b   Arnold, Frances H ^b  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

^b California Institute of Technology   (United States)

Author keywords

(4 nitrobenzyl)pyridine; Alkene epoxidation; P450 BM 3 139 3; Styrene oxide

Indexed keywords

4 (4 NITROBENZYL)PYRIDINE; ALKANE DERIVATIVE; ALKENE; CYTOCHROME P450; CYTOCHROME P450 BM 3; DYE; ENZYME VARIANT; FATTY ACID DERIVATIVE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; STYRENE; STYRENE OXIDE; UNCLASSIFIED DRUG; HYDROGEN PEROXIDE;

ARTICLE; CELL LYSATE; CHEMICAL REACTION; COLORIMETRY; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME METABOLISM; EPOXIDATION; HIGH THROUGHPUT SCREENING; MONITORING; MUTANT; OXIDATION; PRIORITY JOURNAL; SPECTROPHOTOMETRY; VALIDATION PROCESS; CATALYSIS; CHEMICAL STRUCTURE; ESCHERICHIA; GENE VECTOR; GENETICS; METABOLISM; METHODOLOGY; OXIDATION REDUCTION REACTION; PLASMID; VALIDATION STUDY;

ALKENES; CATALYSIS; COLORIMETRY; CYTOCHROME P-450 ENZYME SYSTEM; ESCHERICHIA; GENETIC VECTORS; HYDROGEN PEROXIDE; MODELS, MOLECULAR; MOLECULAR STRUCTURE; OXIDATION-REDUCTION; PLASMIDS; STYRENE;

EID: 16544392183     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/1087057103261913     Document Type: Article

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