Fluorescence microscopy: Established and emerging methods, experimental strategies, and applications in immunology

Microscopy Research and Technique

Volumn 70, Issue 8, 2007, Pages 687-709

  Petty, Howard R ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

Fluorescence; Imaging; Microscopy

Indexed keywords

CAMERAS; ENERGY TRANSFER; FLUORESCENCE IMAGING; FLUORESCENCE MICROSCOPY; IMAGE ENHANCEMENT; IMMUNOLOGY; REFRACTIVE INDEX;

CHANNEL OPENING; CUTTING EDGES; EXPERIMENTAL APPLICATION; EXPERIMENTAL STRATEGY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH SPEED; SINGLE CHANNELS; SINGLE MOLECULE FLUORESCENCE; TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY; TWO PHOTON IMAGING;

SIGNAL TO NOISE RATIO;

FLUORESCENT DYE;

BIOPHYSICS; CAMERA; CONTRAST ENHANCEMENT; DIFFRACTION; EPIFLUORESCENCE MICROSCOPY; FILTRATION; FLUORESCENCE LIFETIME IMAGING; FLUORESCENCE MICROSCOPE; FLUORESCENCE MICROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH SPEED EXPOSURE; HUMAN; IMAGE QUALITY; IMAGING SYSTEM; IMMUNOFLUORESCENCE; IMMUNOLOGY; INSTRUMENTATION; LIGHT TRAP; MICROSCOPE OBJECTIVE; PRIORITY JOURNAL; RAYLEIGH CRITERION; REFRACTION INDEX; REVIEW; SIGNAL DETECTION; SIGNAL NOISE RATIO; SINGLE CHANNEL OPENING EVENT; SINGLE MOLECULE FLUORESCENCE MICROSCOPY; SPECTRAL SENSITIVITY; TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY; TWO PHOTON IMAGING;

EID: 34250182186     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.20455     Document Type: Review

References (112)

1. Abbe E. 1873. Beitrage zur theorie des mikroskops und der mikroskopischen wahrnehmug. Schultze Arch Mikr Anat 9:413.
2. Alivisatos AP, Gu W, Larabell C. 2005. Quantum dots as cellular probes. Ann Rev Biomed Eng 7:55-76.
3. Axelrod D. 2001. Total internal reflection fluorescence microscopy in cell biology. Traffic 2:764-774.
4. Balle B, Farkas DL, Taylor DL, Lanni F. 1993. Enhancement of axial resolution in fluorescence microscopy by standing-wave excitation. Nature 366:44-48.
5. Baschong W, Suetterlin R, Laeng RH. 2001. Control of autofluorescence of archival formaldehyde-fixed, paraffin-embedded tissue in confocal laser scanning microscopy (CLSM). J Histochem Cytochem 49:1565-1571.
6. Baturin AS, Chadaev NN, Leshukov MY, Sheshin EP. 2003. Application of holographic diffusers to improve light uniformity of source with carbon fiber cathodes. J Vac Sci Technol B 21:1629-1632.
7. Bernas T, Zarebski M, Cook RR, Dobrucki JW. 2004. Minimizing photobleaching during confocal microscopy of fluorescent probes bound to chromatin: Role of anoxia and photon flux. J Microscopy 215:281-296.
8. Breeze RH, Ke B. 1972. Some comments on xenon arc lamp stability. Rev Sci Instrum 43:821-823.
9. Broser PJ, Schulte R, Lang S, Rogh A, Helmchen F, Waters J, Sakmann B, Wittum G. 2004. Nonlinear anisotropic diffusion filtering of three-dimensional image data from two-photon microscopy. J Biomed Opt 9:1253-1264.
10. Cappello G, Badoual M, Ott A, Prost J, Busoni L. 2003. Kinesin motion in the absence of external forces characterized by interference total internal reflection microscopy. Phys Rev E Stat Nonlin Soft Matter Phys 68:021907-021917.
11. Chudakov DM, Lukyanov S, Lukyanov KA. 2005. Fluorescent proteins as a toolkit for in vivo imaging. Trends Biotechnol 23:605-613.
12. Clancy B, Cauller LJ. 1998. Reduction of background autofluorescence in brain sections following immersion in sodium borohydride. J Neurosci Methods 83:97-102.
13. Coates CG, Denvir DJ, McHale NG, Thornbury KD, Hollywood MA. 2004. Optimizing low-light microscopy with back-illuminated electron multiplying charge-coupled device: Enhanced sensitivity, speed, and resolution. J Biomed Opt 9:1244-1252.
14. Connally R, Veal D, Piper J. 2002. High resolution detection of fluorescently labeled microorganisms in environmental samples using time-resolved fluorescence microscopy. FEMS Microbiol Ecol 41:239-245.
15. Connally R, Veal D, Pipe J. 2003. Novel flashlamp based time-resolved fluorescence microscope reduces autofluorescence for 30-fold contrast enhancement in environmental samples. In: Conchello JA, Cogswell CJ, Wilson T, editors. Three-dimensional and multidimensional microscopy: Image acquisition and processing X, Vol. 4964. Proceedings of the SPIE. pp 14-23.
16. Connally R, Veal D, Piper J. 2004. Flash lamp-excited time-resolved fluorescence microscope suppresses autofluorescence in water concentrates to deliver an 11-fold increase in signal-to-noise ratio. J Biomed Opt 9:725-734.
17. Cummins CM, Koivunen ME, Stephanian A, Gee SJ, Hammock BD, Kennedy IM. 2006. Application of europium(III) chelate-dyed nanoparticle labels in a competitive atrazine fluoroimmunoassay on an ITO waveguide. Biosens Bioelectron 21:1077-1085.
18. de Haas RR, van Gijlswijk RP, van der Tol EB, Zijlmans HJ, Bakker-Schut T, Bonnet J, Verwoerd NP, Tanke HJ. 1997. Platinum porphyrins as phosphorescent label for time-resolved microscopy. J Histochem Cytochem 45:1279-1292.
19. 2+ flux through individual ion channels with high temporal and spatial resolution. J Biomed Opt 10:11002-11008.
20. Denvir DJ, Conroy E. 2002. Electron multiplying CCD technology: The new ICCD. Presented at SPIE Annual Meeting, Seattle, July.
21. Dittrich PS, Schwille P. 2001. Photobleaching and stabilization of fluorophores used for single-molecule analysis with one- and two-photon excitation. Appl Phys B 73:829-837.
22. Dosev D, Nichkova M, Liu M, Guo B, Liu GY, Hammock BD, Kennedy IM. 2005. Application of luminescent Eu:Gd(2)O(3) nanoparticles to the visualization of protein micropatterns. J Biomed Opt 10:64006.
23. Dussault D, Hoess P. 2004. ICCDs edge out electron-multiplying CCDs in low light. Laser Focus World 40:69-76.
24. Dyba M, Jakobs S, Hell SW. 2003. Immunofluorescence stimulated emission depletion microscopy. Nat Biotechnol 21:1303-1304.
25. Eggeling C, Eolkmer A, Seidel CAM. 2005. Molecular photobleaching kinetics of rhodamine 6G by one- and two-photon induced confocal fluorescence microscopy. Chem Phys Chem 6:791-804.
26. Egner A, Hell SW. 2005. Fluorescence microscopy with super-resolved optical sections. Trends Cell Biol 15:207-215.
27. French T, So PT, Weaver DJ, Jr, Coelho-Sampaio T, Gratton E, Voss EW, Jr, Carrero J. 1997. Two-photon fluorescence lifetime imaging microscopy of macrophage-mediated antigen processing. J Microsc 185:339-353.
28. Gao X, Yang L, Petros JA, Marshall FF, Simons JW, Nie S. 2005. In vivo molecular and cellular imaging with quantum dots. Curr Opin Biotechnol 16:63-72.
29. Goldman RD, Spector DL. 2005. Live cell imaging: A laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
30. Grayson MH, Hotchkiss RS, Karl IE, Holtzman MJ, Chaplin DD. 2003. Intravital microscopy comparing T lymphocyte trafficking to the spleen and the mesenteric lymph node. Am J Physiol Heart Circ Physiol 284:H2213-H2226.
31. Greenbaum L, Rothmann C, Lavie R, Malik Z. 2000. Green fluorescent protein photobleaching: A model for protein damage by endogenous and exogenous singlet oxygen. Biol Chem 381:1251-1258.
32. Griesbeck O. 2004. Fluorescent proteins as sensors for cellular functions. Curr Opin Neurobiol 14:636-641.
33. Gustafsson MG. 2005. Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution. Proc Natl Acad Sci USA 102:13081-13086.
34. Gustafsson MGL. 2000. Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. J Microscopy 198:82-87.
35. 5M: 3D widefield light microscopy with better than 100 nm axial resolution. J Microsc 195:10-16.
36. Hafeman DG, Seul M, Cliffe II CM, McConnell HD. 1984. Superoxide enhances photobleaching during cellular immune attach against fluorescent lipid monolayer membranes. Biochim Biophys Acta 772:20-28.
37. 2+ indicator proteins in transgenic mice under TET control. PLOS Biol 2:763-775.
38. Hell SW. 2004. Toward fluorescence nanoscopy. Nat Biotechnol 21:1347-1355.
39. Hell SW, Wichmann J. 1994. Breaking the diffraction resolution limit by stimulated emission: Stimulated-emission-depletion fluorescence microscopy. Opt Lett 19:780-782.
40. Herman P, Maliwal BP, Lin H-J, Lakowicz JR. 2001. Frequency-domain fluorescence microscopy with the LED as a light source. J Microsc 203:176-181.
41. Herrero FA. 1992. Light-trap design using multiple reflections and solid-angle attenuation: Application to a spaceborne electron spectrometer. Appl Opt 31:5331-5340.
42. Hynecek J, Nishiwaki T. 2003. Excess noise and other important characteristics of low light level imaging using charge multiplying CCDs. IEEE Trans Electronic Dev 50:239-245.
43. Inoue S. 1989. Video enhancement and image processing in light microscopy. Part 1: Video microscopy. Am Lab April:52-60.
44. Inoue S, Inoue T. 1989. Video enhancement and image processing in light microscopy. Part 2: Digital image processing. Am Lab April:62-81.
45. Inoue S, Spring KR. 1997. Video microscopy. New York: Plenum Press. Chapter 13.
46. Jaiswal JK, Simon SM. 2004. Potentials and pitfalls of fluorescent quantum dots for biological imaging. Trends Cell Biol 14:497-504.
47. Johnson GD, de Nogueira C, Araujo GM. 1981. A simple method of reducing the fading of immunofluorescence during microscopy. J Immunol Methods 43:349-350.
48. Johnson GD, Davidson RS, McNamee KC, Russel G, Goodwin D, Holborow EJ. 1982. Fading of immunofluorescence during microscopy: A study of the phenomenon and its remedy. J Immunol Methods 55:231-242.
49. Klar TA, Engel E, Hell SW. 2001. Breaking Abbe's diffraction resolution limit in fluorescence microscopy with stimulated emission depletion beams of various shapes. Phys Rev E 64:066613-1/066613-9.
50. Kindzelskii AL, Sitrin RG, Petty HR. 2004. Optical microspectrophotometry supports the existence of gel phase lipid rafts at the lamellipodium of neutrophils: Apparent role in calcuim signaling. J Immunol 172:4681-4685.
51. Koizumi M, Usui Y. 1972. Fundamental aspects of oxidative and reductive photobleaching of xanthene and thiazine dyes. Mol Photochem 4:57-92.
52. Kong SK, Ko S, Lee CY, Lui PY. 1999. Practical considerations in acquiring biological signals from confocal microscopy. Methods Enzymol 307:20-26.
53. Koopman M, Cambi A, de Bakker BI, Joosten B, Figdor CG, van Hulst NF, Garcia-Parajo MF. 2004. Near-field scanning optical microscopy in liquid for high resolution single molecule detection on dendritic cells. FEBS Lett 573:6-10.
54. Lakowicz, JR. 1983. Principles of fluorescence spectroscopy. New York: Plenum.
55. Lee MF, Kong SK, Fung KP, Lui CP, Lee CY. 1996. Practical considerations in acquiring biological signals from confocal microscope: Solvent effect and temperature effect. Biol Signals 5:291-300.
56. Legg JW, Lewis CA, Parsons M, Ng T, Isacke CM. 2002. A novel PKC-regulated mechanism controls CD44 ezrin association and directional cell motility. Nat Cell Biol 4:399-407.
57. Lewis A, Taha H, Strinkovski A, Manevitch A, Khatchatouriants A, Dekhter R, Ammann E. 2003. Near-field optics: From subwavelength illumination to nanometric shadowing. Nat Biotech 21:1378-1386.
58. Lui PPY, Lee MMF, Ko S, Lee CY, Kong SK. 1997. Practical considerations in acquiring biological signals from confocal microscope. II. Laser-induced rise of fluorescence and effect of agonist droplet application. Biol Signals 6:45-51.
59. Liu T, Liu B, Zhang H, Wang Y. 2005. The fluorescence bioassay plat-forms on quantum dots nanoparticles. J Fluoresc 15:729-733.
60. Magnan P. 2003. Detection of visible photons in CCD and CMOS: A comparative view. Nucl Instrum Methods Phys Res A 504:199-212.
61. Mathies RA, Peck K, Stryer L. 1990. Optimization of high-sensitivity fluorescence detection. Anal Chem 62:1786-1791.
62. Martin G, Agostini HT, Hansen LL. 2005. Light emitting diode microscope illumination for green fluorescent protein isothiocynate epifluorescence. Bio Tech 38:204-206.
63. Mazzini G, Ferrari C, Baraldo N, Mazzini M, Angelini M. 2004. Improvements in fluorescence microscopy allowed by high power light emitting diodes. Curr Issues Multidis Microsc Res Educ 181:8.
64. Meaburn J. 1966. The stability of interference filters. Appl Opt 5:1757-1759.
65. Michalet X, Pinaud FF, Bentolila LA, Tsay JM, Doose S, Li JJ, Sundaresan G, Wu AM, Gambhir SS, Weiss S. 2005. Quantum dots for live cells, in vivo imaging, and diagnostic. Science 307:538-544.
66. Minta A, Kao JP, Tsien RY. 1989. Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores. J Biol Chem 264:8171-8178.
67. 2+ based on green fluorescent proteins and calmodulin. Nature 388:882-887.
68. Moss WC, Haase S, Lyle M, Agard DA, Sedat JW. 2005. A novel 3D wavelet-based filter for visualizing features in noisy biological data. J Microscopy 219:43-49.
69. Neill MAA, Juskaitis R, Wilson T. 1997. Method of obtaining optical sectioning by using structured light in a conventional microscope. Opt Lett 22:1905-1907.
70. Neumann M, Gabel D. 2002. Simple method for reduction of autofluorescence in fluorescence microscopy. J Histochem Cytochem 50:437-439.
71. Nichkova M, Dosev D, Perron R, Gee SJ, Hammock BD, Kennedy IM. 2006. Eu(3+)-doped Gd(2)O(3) nanoparticles as reporters for optical detection and visualization of antibodies patterned by microcontact printing. Anal Bioanal Chem 384:631-637.
72. Nishimura M, Shirasawa H, Song WJ. 2006. A light-emitting diode light source for imaging of neural activities with voltage-sensitive dyes. Neurosci Res 54:230-234.
73. Ober RJ, Martinez C, Lai X, Zhou J, Ward ES. 2004. Exocytosis of IgG as mediated by the receptor, FcRn: An analysis at the single-molecule level. Proc Natl Acad Sci USA 101:11076-11081.
74. Ono M, Murakami T, Kudo A, Isshiki M, Sawada H, Segawa A. 2001. Quantitative comparison of anti-fading mounting media for confocal laser scanning microscopy. J Histochem Cytochem 49:305-311.
75. Panchuk-Voloshina N, Haugland RP, Bishop-Steward J, Bhalgat MK, Millard PJ, Mao F, Leung W-Y, Haugland RP. 1999. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J Histochem Cytochem 47:1179-1188.
76. Patterson GH, Piston DW 2000. Photobleaching in two-photon excitation microscopy. Biophys J 78:2159-2162.
77. Perona P, Malik J. 1990. Scale-space and edge detection using anisotropic diffusion. IEEE Trans Pattern Anal Mach Intell 12:629-639.
78. Piston DW. 1998. Choosing objective lenses: The importance of numerical aperture and magnification in digital microscopy. Biol Bull 195:1-4.
79. Pollack SA. 1966. Angular dependence of transmission characteristics of interference filters and application to a tunable fluorometer. Appl Opt 5:1749-1756.
80. 2+] with genetically encoded Ca2+ indicators. J Neurosci 24:9572-9579.
81. Renikuntla BR, Rose HC, Eldo J, Waggoner AS, Armitage BA. 2004. Improved photostability and fluorescence properties through polyfluorination of a cyanine dye. Org Lett 6:909-912.
82. 2+ dynamics with genetically encoded bioluminescent reporters. Eur J Neurosci 21:597-610.
83. Rulli M, Kuusisto A, Salo J, Kojola H, Simell O. 1997. Time-resolved fluorescence imaging in islet cell autoantibody quantitation. J Immunol Methods 208:169-179.
84. Rumyantsev SL, Shur MS, Bilenko Y, Kosterin PV, Salzberg BM. 2004. Low frequency noise and long-term stability of noncoherent light sources. J Appl Phys 96:966-969.
85. Sabri S, Richelme F, Pierres A, Benoliel A-M, Bongrand P. 1997. Interest of image processing in cell biology and immunology. J Immunol Methods 208:1-27.
86. Safiejko-Mroczka B, Bell PB, Jr. 1996. Bifunctional protein cross-linking reagents improve labeling of cytoskeletal proteins for qualitative and quantitative fluorescence microscopy. J Histochem Cytochem 44:641-656.
87. Salzberg BM, Grinvald A, Cohen LB, Davila HV, Ross WN. 1977. Optical recording of neuronal activity in an invertebrate cenetral nervous system: Simultaneous monitoring of several neurons. J Neurophysiol 40:1281-1291.
88. Schafer-Hales KJ, Belfield KD, Yao S, Frederiksen PK, Hales JM, Kolattukudy PE. 2005. Fluorene-based fluorescent probes with high two-photon action cross-sections for biological multiphoton imaging applications. J Biomed Opt 10:51402-51408.
89. Shaner NC, Steinbach PA, Tsien RY. 2005. A guide to choosing fluorescent proteins. Nat Methods 2:905-910.
90. Smith AM, Gao X, Nie S. 2004a. Quantum dot nanocrystals for in vivo molecular and cellular imaging. Photochem Photobiol 80:377-385.
91. Smith N, Coates C, Giltinan A, Howard J, O'Connor A, O'Driscoll S, Hauser M, Wagner S. 2004b. EMCCD technology and its impact on rapid low-light photometry. In: Garnett J, Beletic JW, editors. Optical and Infrared Detectors for Astronomy, Vol. 5499. Proceedings of SPIE. pp 162-172.
92. Song L, Hennink EJ. Young IT, Tanke HJ. 1995. Photobleaching kinetics of fluorescein in quantitative fluorescence microscopy. Biophys J 68:2588-2600.
93. Song L, Varma CAGO, Verhoeven JW, Tanke HJ. 1996. Influence of the triplet excited state on the photobleaching kinetics of fluorescein in microscopy. Biophys J 70:2959-2968.
94. Song L, van Gijlswijk RPM, Young IT, Tanke HJ. 1997. Influence of fluorochrome labeling density on the photobleaching kinetics of fluorescein in microscopy. Cytometry 27:213-223.
95. Sonoda KH, Nakao S, Nakamura T, Oshima T, Qiao H, Hisatomi T, Kinoshita S, Ishibashi T. 2005. Cellular events in the normal and inflamed cornea. Cornea 24 (Suppl 8):S50-S54.
96. Srivastava M, Jung S, Wilhelm J, Fink L, Buhling F, Welte T, Bohle RM, Seeger W, Lohmeyer J, Maus UA. 2005. The inflammatory versus constitutive trafficking of mononuclear phagocytes into the alveolar space of mice is associated with drastic changes in their gene expression profiles. J Immunol 175:1884-1893.
97. Steinkamp JA, Lehnert NM, Keij JF, Lehnert BE. 1999. Enhanced immunofluorescence measurement resolution of surface antigens on highly autofluroescent, glutaraldehyde-fixed cells analyzed by phase-sensitive flow cytometry. Cytometry 37:275-283.
98. Stern MD, Spurgeon HA, Hansford R, Lakatta EG, Capogrossi MC. 1989. Optimum spectral windows to minimize quantum noise of ratiometric intracellular fluorescent probes. Cell Calcium 10:527-534.
99. Stewart CN, Jr. 2006. Go with the glow: Fluorescent proteins to light transgenic organisms. Trends Biotechnol 24:155-162.
100. Suhling K, French PM, Phillips D. 2005. Time-resolved fluorescence microscopy. Photochem Photobiol Sci 4:13-22.
101. Szmacinski H, Chang Q. 2000. Micro- and sub-nanosecond lifetime measurements using a UV light-emitting diode. Appl Spectrosc 1:106-109.
102. Taylor DL, Wang YL, Heiple JM. 1980. Contractile basis of ameboid movement. VII. The distribution of fluorescently labeled actin in living amebas. J Cell Biol 86:590-598.
103. Theuwissen A, Roks E 2001. Building a better mousetrap: Modified CMOS processes improve image sensor performance. OE Magazine, January 29-32.
104. Treanor B, Lanigan PM, Suhling K, Schreiber T, Munro I, Neil MA, Phillips D, Davis DM, French PM. 2005. Imaging fluorescence life-time heterogeneity applied to GFP-tagged MHC protein at an immunological synapse. J Microsc 217:36-43.
105. Unser M, Aldroubi A. 1996. A review of wavelets in biomedical applications. Proc IEEE 84:626-638.
106. Uttenweiler D, Weber C, Jahne B, Fink RHA, Scharr H. 2003. Spatiotemporal anisotropic diffusion filtering to improve signal-to-noise ratios and object restoration in fluorescence microscopic image sequences. J Biomed Opt 8:40-47.
107. Wallrabe H, Periasamy A. 2005. Imaging protein molecules using FRET and FLIM microscopy. Curr Opin Biotechnol 16:19-27.
108. Yang M, Baranov E, Wang JW, Jiang P, Wang X, Sun FX, Bouvet M, Moossa AR, Penman S, Hoffman RM. 2002. Direct external imaging of nascent cancer, tumor progression, angiogenesis, and metastasis on internal organs in the fluorescent orthotopic model. Proc Natl Acad Sci USA 99:3824-3829.
109. Yu S-J, Kang M-W, Chang H-C, Chen K-M, Yu Y-C. 2005. Bright fluorescent nanodiamonds: No photobleaching and low cytoxicity. J Am Chem Soc 127:17604-17605.
110. Zakrzewski J. 2003. Integrating a spectrometer with an optical microscope presents challenges. SPIE OE Magazine, November, p 29.
111. Zal T, Gascoigne NR. 2004. Using live FRET imaging to reveal early protein-protein interactions during T cell activation. Curr Opin Immunol 16:674-683.
112. Zhao M, Yang M, Baranov E, Wang X, Penman S, Moossa AR, Hoffman RM. 2001. Spatial-temporal imaging of bacterial infection and antibiotic response in intact animals. Proc Natl Acad Sci USA 98:9814-9818.