Photobleaching in two-photon excitation microscopy

Biophysical Journal

Volumn 78, Issue 4, 2000, Pages 2159-2162

  Patterson, George H ^a   Piston, David W ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COUMARIN DERIVATIVE; FLUORESCEIN; INDO 1; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

ARTICLE; BLEACHING; EXCITATION; FLUORESCENCE; IRRADIATION; LASER; MICROSCOPY; PHOTON;

EID: 0034110795     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(00)76762-2     Document Type: Article

References (14)

1. Centonze, V. E., and J. G. White. 1998. Multiphoton excitation provides optical sections from deeper within scattering specimens than confocal imaging. Biophys. J. 75:2015-2024.
2. Denk, W., D. W. Piston, and W. W. Webb. 1995. Two-photon molecular excitation in laser-scanning microscopy. In Handbook of Biological Confocal Microscopy. J. B. Pawley, editor. Plenum Press, New York. 445-458.
3. Denk, W., J. H. Strickler, and W. W. Webb. 1990. Two-photon laser scanning fluorescence microscopy. Science. 248:73-76.
4. Denk, W., and K. Svoboda. 1997. Photon upmanship: why multiphoton imaging is more than a gimmick. Neuron. 18:351-357.
5. Heikal, A. A., and W. W. Webb. 1999. One-and two-photon time-resolved fluorescence spectroscopy of selected fluorescent markers: photobleaching, triplet-, and singlet-state dynamics. Biophys. J. 76:260a. (Abstr.).
6. Patterson, G. H., S. M. Knobel, W. D. Sharif, S. R. Kain, and D. W. Piston. 1997. Use of the green-fluorescent protein (GFP) and its mutants in quantitative fluorescence microscopy. Biophys. J. 73:2782-2790.
7. Piston, D. W., B. D. Bennett, and G. Ying. 1995. Imaging of cellular dynamics by two-photon excitation microscopy. Microsc. Microanal. 1:25-34.
8. Piston, D. W., and S. M. Knobel. 1999. Quantitative imaging of metabolism by two-photon excitation microscopy. Methods Enzymol. 307: 351-368.
9. Sánchez, E. J., L. Novotny, G. R. Holtom, and X. S. Xie. 1997. Room-temperature fluorescence imaging and spectroscopy of single molecules by two-photon excitation. J. Phys. Chem. A. 101:7019-7023.
10. Song, L., E. J. Hennik, I. T. Young, and H. J. Tanke. 1995. Photobleaching kinetics of fluorescein in quantitative fluorescence microscopy. Biophys. J. 68:2588-2600.
11. Song, L., C. A. G. O. Varma, J. W. Verhoeven, and H. J. Tanke. 1996. Influence of the triplet excited state on the photobleaching kinetics of fluoroscein in microscopy. Biophys. J. 70:2959-2968.
12. Tsien, R. Y., and A. Waggoner. 1995. Fluorophores for confocal microscopy: photophysics and photochemistry. In Handbook of Biological Confocal Microscopy. J. B. Pawley, editor. Plenum Press, New York. 267-279.
13. Widengren, J., and R. Rigler. 1996. Mechanisms of photobleaching investigated by fluorescence correlation spectroscopy. Bioimaging. 4:149-157.
14. Xu, C., R. M. Williams, W. Zipfel, and W. W. Webb. 1996. Multiphoton excitation cross-sections of molecular fluorophores. Bioimaging. 4:198-207.