Optimizing low-light microscopy with back-illuminated electron multiplying charge-coupled device: Enhanced sensitivity, speed, and resolution

Journal of Biomedical Optics

Volumn 9, Issue 6, 2004, Pages 1244-1252

  Coates, Colin G ^a   Denvir, Donal J ^a   McHale, Noel G ^b   Thornbury, Keith D ^b   Hollywood, Mark A ^b  

^a OXFORD INSTRUMENTS PLASMA TECHNOLOGY   (United Kingdom)

^b QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

Author keywords

Back illuminated charge coupled device; Calcium flux microscopy; Charge coupled device; Electron multiplying charge coupled device; Impact ionization; Intensified charge coupled device; Intracellular ion signaling; Quantum efficiency; Single molecule detection; Single photon sensitivity

Indexed keywords

CAMERAS; CHARGE COUPLED DEVICES; ELECTRON MULTIPLIERS; IMPACT IONIZATION; OPTIMIZATION; PHOTONS; QUANTUM EFFICIENCY; SPURIOUS SIGNAL NOISE;

BACK ILLUMINATED CHARGE COUPLED DEVICE; CALCIUM FLUX MICROSCOPY; ELECTRON MULTPLYING CHARGE COUPLED DEVICE; INTENSIFIED CHARGE COUPLED DEVICE; INTRACELLULAR ION SIGNALLING; SINGLE MOLECULE DETECTION; SINGLE PHOTON SENSITIVITY;

OPTICAL MICROSCOPY;

CALCIUM; DIAGNOSTIC AGENT;

ANIMAL; ARTICLE; CALCIUM SIGNALING; CELL CULTURE; COMPARATIVE STUDY; CONFOCAL MICROSCOPY; CYTOLOGY; ELECTRON; EQUIPMENT; EQUIPMENT DESIGN; EVALUATION; FLUORESCENCE MICROSCOPY; GUINEA PIG; IMAGE ENHANCEMENT; INSTRUMENTATION; METABOLIC CLEARANCE RATE; METABOLISM; METHODOLOGY; PHYSIOLOGY; RABBIT; SIGNAL PROCESSING; VALIDATION STUDY; VASCULAR SMOOTH MUSCLE;

ANIMALS; CALCIUM; CALCIUM SIGNALING; CELLS, CULTURED; ELECTRONS; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; GUINEA PIGS; IMAGE ENHANCEMENT; METABOLIC CLEARANCE RATE; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MUSCLE, SMOOTH, VASCULAR; RABBITS; SIGNAL PROCESSING, COMPUTER-ASSISTED;

EID: 12844266284     PISSN: 10833668     EISSN: 15602281     Source Type: Journal    
DOI: 10.1117/1.1805559     Document Type: Article

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