Two-photon fluorescence lifetime imaging microscopy of macrophage-mediated antigen processing

Journal of Microscopy

Volumn 185, Issue 3, 1997, Pages 339-353

  French, T ^a   So, P T C ^a   Weaver, D J ^b   Coelho Sampaio, T ^c   Gratton, E ^a   Voss, E W ^b   Carrero, J ^b  

^a University of Illinois at Urbana Champaign   (United States)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^c FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

Author keywords

Antigen processing; FITC BSA; Fluorescence; Fluorescence lifetime imaging microscopy (FLIM); Macrophage; Two photon

Indexed keywords

AMINO ACIDS; ANTIGEN-ANTIBODY REACTIONS; ANTIGENS; BODY FLUIDS; DEXTRAN; ENZYME ACTIVITY; FLUORESCENCE IMAGING; LACTIC ACID; MOLECULAR BIOLOGY; PHOTONS;

ANTIGEN PROCESSING; BOVINE SERUM ALBUMINS; FITC-BSA; FLUORESCENCE LIFETIME IMAGING MICROSCOPY; FLUORESCENCE LIFETIMES; NATIVE STATE; TIME-DEPENDENT CHANGES; TWO PHOTON; TWO-PHOTON FLUORESCENCE LIFETIME;

MACROPHAGES;

ANTIGEN;

ARTICLE; FLOW CYTOMETRY; FLUORESCENCE; IMAGING; MICROSCOPY; PRIORITY JOURNAL;

BOVINAE;

EID: 0030998105     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1997.d01-632.x     Document Type: Article

References (60)

1. Aubry, L., Klein, G., Martiel., J.-L. & Satre, M. (1993) Kinetics of endosomal pH evolution in Dictyostelium discoideum amoebae. J. Cell. Sci. 105, 861-866.
2. Bailey, E.A. & Rollefson, G.K. (1953) Determination of fluorescence lifetimes of dissolved substances by a phase shift method. J. Chem. Phys. 21, 1315-1322.
3. Benjamin, D.C., Berzofsky, J.A., East, I.J., Curd, F.R.N., Hannum, C., Leach, S.J., Margoliash, E., Michae, J.G., Miller, A., Prager, E.M., Reichlin, M., Sercarz, E.E., Smith-Gill, S.J., Todd, P.E. & Wilson, A.C. (1984) The antigenic structure of proteins: a reappraisal. Ann. Rev. Immunol. 2, 67-101.
4. Brown, J.H., Jardetzky, T.S., Gorga, J.C., Stern, L.J., Urban, R.G., Strominger, J.L. & Wiley, D.C. (1993) Three-dimensional structure of the human Class II histocompatibility antigen HLA-DR1. Nature, 364, 33-39.
5. Buurman, E.P., Sanders, R., Draaijer, A., Gerritsen, H.C., van, Veen, J.J.F., Houpt, P.M. & Levine, Y.K. (1992) Fluorescence lifetime imaging using a confocal laser scanning microscope. Scanning, 14, 155-159.
6. Collins, D.S., Unanue, E.R. & Harding, C.V. (1991) Reduction of disulfide bonds within lysosomes is a key step in antigen processing. J. Immunol. 147, 4054-4059.
7. Cresswell, P. (1994) Assembly, transport, and function of MHC Class II molecules. Ann. Rev. Immunol. 12, 259-293.
8. Creighton, T.E. (1993) Proteins: Structures and Molecular Properties. W.H. Freeman and Company, New York.
9. Denk, W., Strickler, J.H. & Webb, W.W. (1990) Two-photon laser scanning fluorescence microscopy. Science, 24, 73-76.
10. Diment, S. (1990) Different roles for thiol and aspartyl protease in antigen presentation of ovalbumin. J. Immunol. 142, 2221-2229.
11. Diment, S., Martin, K.J. & Stahl, P.D. (1989) Cleavage of parathyroid hormone in macrophage endosomes illustrates a novel pathway for intracellular processing of proteins. J. Biol. Chem. 264, 13403-13406.
12. Diment, S. & Stahl, P. (1985) Macrophage endosomes contain proteases which degrade endocytosed protein ligands. J. Biol. Chem. 260, 15311-15317.
13. Dix, J.A. & Verkman, A.S. (1990) Pyrene excimer mapping in cultured fibroblasts by ratio imaging and time-resolved microscopy. Biochemistry, 29, 1949-1953.
14. Dong, C.Y., So, P.T.C., French, T. & Gratton, E. (1995) Fluorescence lifetime imaging by asynchronous pump-probe microscopy. Biophys. J. 69, 2234-2242.
15. Gadella, T.W.J. Jr & Jovin, T.M. (1995) Oligomerization of epidermal growth factor receptors on A431 cells studied by time-resolved fluorescence imaging microscopy. A stereochemical model for tyrosine kinase receptor activation. J. Cell Biol. 129, 1543-1548.
16. Germain, R.N. & Margulies, D.H. (1993) The biochemistry and cell biology of antigen processing and presentation. Ann. Rev. Immunol. 11, 403-450.
17. Gratton, E. & Limkeman, M. (1983) A continuously variable frequency cross correlation phase fluorometer with picosecond resolution. Biophys. J. 44, 315-324.
18. Green, J.M., Demars, R., Xu, X. & Peirce, S.K. (1995) The intracellular transport of MHC II molecules in the absence of HLA-DM. J. Immunol. 155, 3759-3768.
19. Gu, M. & Sheppard, C.J.R. (1995) Comparison of three-dimensional imaging properties between two-photon and single-photon fluorescence microscopy. J. Microsc. 177, 128-137.
20. Hardings, C.V., Collins, D.S., Slot, J.W., Geuze, H.J. & Unanue, E.R. (1991) Liposome-encapsulated antigens are processed in lysosomes, recycled and presented to T cells. Cell, 64, 393-401.
21. Hardings, C.V. & Geuze, H.J. (1992) Class II MHC molecules are present in macrophage liposomes and phagoliposomes that function in the phagocytic processing of Listeria monocytogenes for presentation to T cells. J. Cell Biol. 119, 531-542.
22. He, X.-M. & Carter, D.C. (1992) Atomic structure and chemistry of human serum albumin. Nature, 358, 209-215.
23. Jensen, P. (1993) Acidification and disulfide bond reduction can be sufficient to allow intact proteins to bind to class II. J. Immunol. 150, 3347-3356.
24. Keating, S.M. & Wensel, T.G. (1990) Nanosecond fluorescence microscopy: emission kinetics of Fura-2 in single cells. Biophys. J. 59, 186-202.
25. Kuby, J. (1994) Immunology, pp. 243-245. W. H. Freeman & Co., New York.
26. Lakowicz, J.R., Szmacinski, H., Lederer, W.J., Kirby, M.S., Johnson, M.L. & Nowaczyk, K. (1994) Fluorescence lifetime imaging of intracellular calcium in COS cells using QUIN-2. Cell Calcium, 15, 7-27.
27. Levine, T.P & Chain, B.M. (1991) The cell biology of antigen processing. Crit. Rev. Biochem. Mol. Biol. 26, 439-473.
28. Marks, M.S., Blum, J.S. & Cresswell (1990) Invarient chain trimers are sequestered in the rough endoplasmic reticulum in the absence of association with HLA class II antigens. J. Cell Biol. 111, 839-855.
29. Martin, M.M. & Lindqvist, L. (1975) The pH dependence of fluorescein fluorescence. J. Luminesc. 10, 381-390.
30. Maurer, P.H. (1965) Antigenicity of polypeptides [poly alpha-amino acids]. Immunological studies with synthetic polymers containing only D- or D- and L- alpha amino acids. J. Exp. Med. 121, 339.
31. Mego, J.L. (1984) Role of thiols, pH and cathepsin D in the lysosomal catabolism of serum albumin. Biochem. J. 218, 775-783.
32. Morgan, C.G., Mitchell, A.C. & Murray, J.G. (1992) Prospects for confocal imaging based on nanosecond fluorescence decay time. J. Microsc. 165, 49-60.
33. Müller, M., Ghauharali, R., Visscher, K. & Brakenhoff, G. (1995) Double pulse fluorescence lifetime imaging in confocal microscopy. J. Microsc. 177, 171-179.
34. Murphy, R.F., Powers, S. & Cantor, C.R. (1984) Endosome pH measured in single cells by dual fluorescence flow cytometry: rapid acidification of insulin to pH 6. J. Cell. Biol. 98, 1757-1762.
35. Nakamura, O. (1992) Three-dimensional imaging characteristics of laser scan fluorescence microscopy: Two-photon excitation vs. single-photon excitation. Optik, 93, 93-42.
36. Neefjes, J.J. & Ploegh, H.L. (1992) Intracellular transport of MHC class II molecules. Immunol. Today, 13, 179-184.
37. Ohkuma, S. & Poole, B. (1978) Fluorescence probe measurement of the intralysosomal pH in living cells and the perturbation of pH in various agents. Proc. Natl. Acad. Sci. USA, 75, 3327-3331.
38. Oida, T., Sako, Y. & Kusumi, A. (1993) Fluorescence lifetime imaging microscopy (flimscopy). Biophys. J. 64, 676-685.
39. Perrin, F. (1929) La fluorescence des solutions. Ann. Phys. 12, 169-175.
40. Piston, D.W., Kirby, M.S., Cheng, H., Ledere, W.J. & Webb, W.W. (1994) Two photon excitation fluorescence imaging of three-dimensional calcium ion activity. Appl. Opt. 33, 662-669.
41. Piston, D.W., Sandison, D.R. & Webb, W.W. (1992) Time-resolved fluorescence imaging and background rejection by two-photon excitation in laser scanning microscopy. Proc. SPIE, 1640, 379-389.
42. Puri, J.P. & Factorovich, Y. (1988) Selective inhibition of antigen presentation to cloned T cells by protease inhibitors. J. Immunol. 141, 3313-3317.
43. Qui, Y., Xu, X., Wandinger-Ness, A., Dalke, D.P. & Pierce, S.K. (1994) Separation of subcellular compartments containing distinct functional forms of MHC II. J. Cell. Biol. 125, 595-605.
44. Sadegh-Nasseri, S. (1994) Peptide, invariant chain, or molecular aggregation preserves class II from functional inactivation. Antigen Processing and Presentation (ed. by R. E. Humphreys and S. K. Pierce), pp. 171-187. Academic Press, Inc. San Diego.
45. Sanders, R., Draaijer, A., Gerritsen, H.C., Houpt, P.M. & Levine, Y.K. (1995a) Quantitative pH imaging in cells using confocal fluorescence lifetime imaging microscopy. Anal. Biochem. 227, 302-308.
46. Sanders, R., Draaijer, A., Gerritsen, H.C. & Levine, Y.K. (1995b) Selective imaging of multiple probes using fluorescence lifetime contrast. Zool. Stud. 34, 173-174.
47. Schwartz, A.L. (1990) Cell biology of intracellular protein trafficking. Ann. Rev. Immunol. 8, 195-229.
48. Sela, M. (1966) Immunological studies with synthetic polypeptides. Adv. Immunol. 5, 30.
49. Sheppard, C.J.R. & Gu, M. (1990) Image formation in two-photon fluorescence microscopy. Optik, 86, 104-106.
50. So, P.T.C., French, T., Yu, W.M., Berland, K.M., Dong, C.Y. & Gratton, E. (1995) Two-photon fluorescence microscopy: time-resolved and intensity imaging. Bioimaging, 3, 49-63.
51. Straubinger, R.M., Papahadjopoulos, D. & Hong, K. (1990) Endocytosis and intracellular fate of liposomes using pyranine as a probe. Biochemistry, 29, 4929-4939.
52. Takahashi, H., Cease, K.B. & Berzofsky, J.A. (1989) Identification of protease that process distinct epitopes on the same protein. J. Immunol. 142, 2221-2229.
53. Tycko, B. & Maxfield, F.R. (1982) Rapid acidifcation of endocytic vesicles containing α2-macroglobulin. Cell, 28, 643-655.
54. Voss, E.W. Jr (1984) Immunological properties of fluorescein. Fluorescein Hapten: An Immunological Probe (ed. by E. W. Voss Jr), pp. 5-14. CRC press, Inc., Boca Raton, FL.
55. Voss, E.W. Jr (1990) Anti-fluorescein antibodies as structure-function models to examine fundamental immunological and spectroscopic principles. Commun. Mol. Cell. Biophys. 6, 197-221.
56. Voss, E.W. Jr, Workman, C.J. & Mummert, M.E. (1996) Detection of protease activity using a fluorescence-enhancement globular substrate. BioTechniques, 20, 286-291.
57. Weaver, D.J. Jr, Cherukuri, A., Carrero, J., Coehlo-Sampaio, T., Durack, G. & Voss, E.W. Jr. (1996) Macrophage mediated processing of an exogenous fluorescent antigenic probe: time dependent elucidation of the processing pathway. Biol. Cell. 87, 95-104.
58. Weaver, D.J. Jr, Durack, G. & Voss, E.W. Jr (1997) Analysis of the intracellular processing of proteins: application of a novel fluorescent probe and fluorescence polarization. Cytometry, in press.
59. Wilson, T. & Sheppard, C. (1984) Theory and Practice of Scanning Optical Microscopy. Academic Press, New York.
60. Xu, X., Wenxia, S., Cho, H., Qui, Y. & Peirce, S. (1995) Intracellular transport of invarient chains-MHC class II complexes to the peptide loading compartment. J. Immunol. 155, 2984-2992.