Determining Actinobacillus succinogenes metabolic pathways and fluxes by NMR and GC-MS analyses of 13C-labeled metabolic product isotopomers

Metabolic Engineering

Volumn 9, Issue 2, 2007, Pages 177-192

  McKinlay, James B ^a   Shachar Hill, Yair ^b   Zeikus, J Gregory ^a,c   Vieille, Claire ^c  

^a Michigan State University ^*  (United States)

^b MICHIGAN STATE UNIVERSITY   (United States)

^c Michigan State University   (United States)

Author keywords

Actinobacillus succinogenes; Biomass composition; Malic enzyme; Metabolic flux analysis; Oxaloacetate decarboxylase; Succinate; Transhydrogenase

Indexed keywords

AMINO ACIDS; BACTERIA; GAS CHROMATOGRAPHY; GLUCOSE; MASS SPECTROMETRY; METABOLISM; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

ACTINOBACILLUS SUCCINOGENES; BIOMASS COMPOSITION; MALIC ENZYME; METABOLIC FLUX ANALYSIS; OXALOACETATE DECARBOXYLASE; TRANSHYDROGENASE;

GENETIC ENGINEERING;

AMINO ACID; CARBOXYLIC ACID; CELL EXTRACT; FORMIC ACID; MALATE DEHYDROGENASE (DECARBOXYLATING); MALIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) TRANSHYDROGENASE; OXALOACETIC ACID; PENTOSE PHOSPHATE; PYRUVATE DEHYDROGENASE; PYRUVIC ACID; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SUCCINIC ACID;

ACTINOBACILLUS; ACTINOBACILLUS SUCCINOGENE; ARTICLE; BACTERIAL METABOLISM; BIOMASS; CARBON NUCLEAR MAGNETIC RESONANCE; CELL GROWTH; CONTROLLED STUDY; DECARBOXYLATION; ENZYME ACTIVITY; EXPERIMENTAL STUDY; FERMENTATION; GAS CHROMATOGRAPHY; GLUCOSE INTAKE; IN VIVO STUDY; MASS SPECTROMETRY; METABOLIC ENGINEERING; METABOLISM; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PENTOSE PHOSPHATE CYCLE; PRIORITY JOURNAL;

ACTINOBACILLUS; CARBON ISOTOPES; COMPUTER SIMULATION; ENERGY METABOLISM; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GENE EXPRESSION PROFILING; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, BIOLOGICAL; MULTIENZYME COMPLEXES; PROTEOME; SIGNAL TRANSDUCTION;

ACTINOBACILLUS SUCCINOGENES;

EID: 33847326777     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2006.10.006     Document Type: Article

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