Elucidation of anaplerotic pathways in Corynebacterium glutamicum via 13C-NMR spectroscopy and GC-MS

Applied Microbiology and Biotechnology

Volumn 47, Issue 4, 1997, Pages 430-440

  Park, S M ^a   Shaw Reid, C ^a   Sinskey, A J ^b   Stephanopoulos, G ^a  

^a Massachusetts Institute of Technology Cambridge   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; ASPARTIC ACID; GLUCONIC ACID; PYRUVIC ACID;

ARTICLE; BACTERIAL METABOLISM; BIOENGINEERING; CARBON DIOXIDE FIXATION; CONTROLLED STUDY; CORYNEBACTERIUM GLUTAMICUM; ENZYME ACTIVITY; MASS SPECTROMETRY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

EID: 0031009769     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530050952     Document Type: Article

References (24)

1. Brewster NK, Val DL, Walker ME, Wallace JC (1994) Regulation of pyruvate carboxylase isozyme (PYC1, PYC2) gene expression in Saccharomyces cerevisiae during fermentative and non-fermentative growth. Arch Biochem Biophys 311: 62-71
2. 13C NMR studies in perfused rat hearts. J Biol Chem 258: 13 785-13 794
3. Gubler M, Park SM, Jetten M, Stephanopoulos G, Sinskey AJ (1994) Effects of phosphoenolpyruvate carboxylase deficiency on metabolism and lysine production in Coryncbacterium glutamicum. Appl Microbiol Biotechnol 40: 857-863
4. 2-fixing enzymes in Pseudomonas fluorescens. J Gen Microbiol 93: 63-74
5. Hoffmann KH. Mustafa T, Jorgensen JB (1979) Role of pyruvate kinase, phosphoenolpyruvate carboxykinase, malic enzyme and lactate dehydrogenase in anaerobic energy metabolism of Tubifex spec. J Comp Physiol 130: 337-345
6. 13C-enriched L-lysine by Brevibacterium flavum. Eur J Biochem 162: 621-633
7. 13C-n.m.r. investigation of the metabolism of amino acids in renal proximal convoluted tubules of normal and streptozotocin-treated rats and rabbits. Biochem J 263: 231-241
8. Jetten MSM, Pitoc GA, Follettie MT, Sinskey AJ (1994) Regulation of phospho(enol)-pyruvate- and oxaloacetate-converting enzymes in Corynebacterium glutamicum. Appl Microbiol Biotechnol 41: 47-52
9. Krulwich TA, Pelliccione NJ (1979) Catabolic pathways of coryneforms, nocardias, and mycobacteria. Annu Rev Microbiol 33: 95-111
10. O'Brien RW, Chuang DT, Taylor BL, Utter MF (1977) Novel enzymic machinery for the metabolism of oxaloacetate, phosphoenolpyruvate, and pyruvate in Pseudomonas citronellolis. J Biol Chem 252: 1257-1263
11. Park SM (1996) Investigation of carbon fluxes in central carbon metabolic pathways of C. glutamicum. PhD thesis, Massachusetts Institute of Technology
12. Park SM, Sinskey AJ, Stephanopoulos G (1996). Application of metabolic flux analysis to metabolic and physiological studies of Corynebacterium glutamicum. (in press)
13. Peters-Wendisch PG, Eikmanns BJ, Thierbach G, Bachmann B, Sahm H (1993). Phosphoenolpyruvate carboxylase in Corynebacterium glutamicum is dispensable for growth and lysine production. FEMS Microbiol Lett 112: 269-274
14. Schrumpf B, Eggeling L, Sahm H (1992) Isolation and prominent characteristics of a L-lysine hyperproducing strain of Corynebacterium glutamicum. Appl Microbiol Biotechnol 37: 566-571
15. Scrutton MC, Taylor BL (1974) Isolation and characterization of pyruvate carboxylase from Azotobacter vinelandii OP. Arch Biochem Biophys 164: 641-654
16. Shiio I, Yoshino H, Sughnoto S (1990) Isolation and properties of lysine-producing mutants with feedback-resistant aspartokinase derived from a Brevibacterium flavum strain with citrate synthase- and pyruvate-kinase-defects and feedback-resistant phosphoenolpyruvate carboxylase. Agric Biol Chem 54: 3275-3282
17. 1H-NMR spectroscopy. Eur J Biochem 213: 1325-1331
18. Toh H, Kawamura T, Izui K (1994) Molecular evolution of phosphoenolpyruvate carboxylase. Plant Cell Environ 17: 31-43
19. Tosaka O, Morioka H, Takinami K (1979) The role of biotin-dependent pyruvate carboxylase in L-lysine production. Agric Biol Chem 43: 1513-1519
20. Vallino JJ, Stephanopoulos G (1993) Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine overproduction. Biotechnol Bioeng 41: 633-646
21. 13C nuclear magnetic resonance studies of the biosynthesis by Microbacterium ammoniaphilum of L-glutamate selectively enriched with carbon-13. J Biol Chem 257: 1189-1195
22. Wurtele ES, Nikolau BJ (1990) Plants contain multiple enzymes: discovery of 3-methylcrotonyl-CoA carboxylase, propionyl-CoA carboxylase and pyruvate carboxylase in the plant kingdom. Arch Biochem Biophys 278: 179-186
23. 13C-NMR studies of lysine fermentation with a Corynebacterium glutamicum mutant. Agric Biol Chem 50: 2453-2459
24. Yokota A, Shiio I (1988) Effects of reduced citrate synthase activity and feedback-resistant phosphoenol pyruvate carboxylase on lysine productivities of Brevibacterium flavum mutants. Agric Biol Chem 52: 455-463