A human hepatocellular in vitro model to investigate steatosis

Chemico-Biological Interactions

Volumn 165, Issue 2, 2007, Pages 106-116

  Gómez Lechón, María José ^a   Donato, María Teresa ^a,b   Martínez Romero, Alicia ^c   Jiménez, Nuria ^a   Castell, José Vicente ^a,b   O'Connor, José Enrique ^b,c  

^a HOSPITAL UNIVERSITARIO LA FE   (Spain)

^b UNIVERSITY OF VALENCIA   (Spain)

^c CENTRO DE INVESTIGACIÓN PRÍNCIPE FELIPE   (Spain)

Author keywords

Apoptosis; Cellular steatosis; Fatty acids; Hepatocytes; HepG2

Indexed keywords

FATTY ACID; OLEIC ACID; PALMITIC ACID;

ANALYTIC METHOD; APOPTOSIS; ARTICLE; CELL CULTURE; COMPARATIVE STUDY; CONTROLLED STUDY; CYTOTOXICITY; FATTY LIVER; HUMAN; HUMAN CELL; LIPID STORAGE; LIVER CELL; NONALCOHOLIC FATTY LIVER;

APOPTOSIS; CARCINOMA, HEPATOCELLULAR; CELL LINE, TUMOR; CELL SURVIVAL; DOSE-RESPONSE RELATIONSHIP, DRUG; FATTY ACIDS, NONESTERIFIED; FATTY LIVER; HEPATOCYTES; HUMANS; NEUTRAL RED; OLEIC ACID; PALMITIC ACID;

EID: 33846106076     PISSN: 00092797     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbi.2006.11.004     Document Type: Article

References (41)

1. Gasbarrini G., Vero V., Miele L., Forgione A., Hernandez A.P., Greco A.V., Gasbarrini A., and Grieco A. Nonalcoholic fatty liver disease: defining a common problem. Eur. Rev. Med. Pharmacol. Sci. 9 (2005) 253-259
2. Bradbury M.W. Lipid metabolism and liver inflammation. I. Hepatic fatty acid uptake: possible role in steatosis. Am. J. Physiol. Gastrointest. Liver Physiol. 290 (2006) G194-G198
3. Mulhall B.P., Ong J.P., and Younossi Z.M. Non-alcoholic fatty liver disease: an overview. J. Gastroenterol. Hepatol. 17 (2002) 1136-1143
4. Unger R.H., and Orci L. Lipoapoptosis: its mechanism and its diseases. Biochim. Biophys. Acta 1585 (2002) 202-212
5. Powell E.E., Jonsson J.R., and Clouston A.D. Steatosis: co-factor in other live diseases. Hepatology 42 (2005) 5-13
6. Wang D., Wei Y., and Pagliassotti M.J. Saturated fatty acids promote endoplasmic reticulum stress and liver injury in rats with hepatic steatosis. Endocrinology 147 (2006) 943-951
7. Koteish A., and Diehl A.M. Animal models of steatosis. Semin. Liver Dis. 21 (2001) 89-104
8. Lieber C.S. Alcoholic fatty liver: its pathogenesis and mechanism of progression to inflammation and fibrosis. Alcohol 34 (2004) 9-19
9. Feldstein A.E., Canbay A., Guicciardi M.E., Higuchi H., Bronk S.F., and Gores G.J. Diet associated hepatic steatosis sensitizes to Fas mediated liver injury in mice. J. Hepatol. 39 (2003) 978-983
10. Donato M.T., Lahoz A., Jiménez N., Pérez G., Serralta A., Mir J., Castell J.V., and Gómez-Lechón M.J. Potential impact of steatosis on cytochrome P450 enzymes of human hepatocytes isolated from fatty liver grafts. Drug Metab. Disp. 34 (2006) 1556-1562
11. Malhi H., Bronk S.F., Werneburg N.W., and Gores G.J. Free fatty acids induce JNK-dependent hepatocyte lipoapoptosis. J. Biol. Chem. 281 (2006) 12093-12101
12. Feldstein A.E., Canbay A., Angulo P., Taniai M., Burgart L.J., Lindor K.D., and Gores G.J. Hepatocyte apoptosis and fas expression are prominent features of human nonalcoholic steatohepatitis. Gastroenterology 125 (2003) 437-443
13. Gomez-Lechon M.J., Donato M.T., Castell J.V., and Jover R. Human hepatocytes as a tool for studying toxicity and drug metabolism. Curr. Drug Metab. 4 (2003) 292-312
14. Gomez-Lechon M.J., Donato M.T., Castell J.V., and Jover R. Human hepatocytes in primary culture: the choice to investigate drug metabolism in man. Curr. Drug Metab. 5 (2004) 443-462
15. Malhi H., Gores G.J., and Lemasters J.J. Apoptosis and necrosis in the liver: a tale of two deaths?. Hepatology 43 (2006) S31-S44
16. Araya J., Rodrigo R., Videla L.A., Thielemann L., Orellana M., Pettinelli P., and Poniachik J. Increase in long-chain polyunsaturated fatty acid n - 6/n - 3 ratio in relation to hepatic steatosis in patients with non-alcoholic fatty liver disease. Clin. Sci. 106 (2004) 635-643
17. Greenspan P., and Fowler S.D. Spectrofluorometric studies of the lipid probe, Nile Red. J. Lipid Res. 26 (1985) 781-789
18. McMillian M.K., Grand E.R., Zhong A., Parker J.B., Li L., Zivin R.A., Burczynski M.E., and Johnson M.D. Nile Red binding to HepG2 cells: an improved assay for in vitro studies of hepatosteatosis. In Vitro Mol. Toxicol. 14 (2001) 177-190
19. Gomez-Lechon M.J., Ponsoda X., O'Connor E., Donato M.T., Castell J.V., and Jover R. Diclofenac induces apoptosis in hepatocytes by alteration of mitochondrial function and generation of ROS. Biochem. Pharmacol. 66 (2003) 2155-2167
20. Amacher D.E., and Martin B.A. Tetracycline-induced steatosis in primary canine hepatocyte cultures. Fundam. Appl. Toxicol. 40 (1997) 256-263
21. Babich H., and Borenfreund E. Cytotoxic effects of food additives and pharmaceuticals on cells in culture as determined with the neutral red assay. J. Pharm. Sci. 79 (1990) 592-594
22. Vermes I., Haanen C., and Reutelingsperger C. Flow cytometry of apoptotic cell death. J. Immunol. Methods 243 (2000) 167-190
23. Gomez-Lechón M..J., O'Connor E., Castell J.V., and Jover R. Sensitive markers used to identify compounds that trigger apoptosis in cultured hepatocytes. Toxicol. Sci. 65 (2002) 299-308
24. Browning J.D., and Horton J.D. Molecular mediators of hepatic steatosis and liver injury. J. Clin. Invest. 114 (2004) 147-152
25. Hoekstra R., and Chamuleau R.A. Recent developments on human cell lines for the bioartificial liver. Int. J. Artif. Organs 25 (2002) 182-191
26. Yamashita R., Saito T., Satoh S., Aoki K., Kaburagi Y., and Sekihara H. Effects of dehydroepiandrosterone on gluconeogenic enzymes and glucose uptake in human hepatoma cell line, HepG2. Endocr. J. 52 (2005) 727-733
27. Shimomura I., Shimano H., Korn B.S., Bashmakov Y., and Horton J.D. Nuclear sterol egulatory element-binding proteins activate genes responsible for the entire program of saturated fatty acid biosynthesis in transgenic mouse liver. J. Biol. Chem. 273 (1998) 35299-35306
28. Sato H., Mohamed T., Goto A., Oikawa S., and Kurosawa T. Fatty acid profiles in relation to triglyceride level in the liver of dairy cows. J. Vet. Med. Sci. 66 (2004) 85-87
29. Listenberger L.L., Han X., Lewis S.E., Cases S., Farese Jr. R.V., Ory D.S., and Schaffer J.E. Triglyceride accumulation protects against fatty acid-induced lipotoxicity. Proc. Natl. Acad. Sci. U.S.A. 100 (2003) 3077-3082
30. Gocze P.M., and Freeman D.A. Factors underlying the variability of lipid droplet fluorescence in MA-10 Leydig tumor cells. Cytometry 17 (1994) 151-158
31. Unger R.H. The physiology of cellular liporegulation. Annu. Rev. Physiol. 65 (2003) 333-347
32. Farrell G.C., and Larter C.Z. Nonalcoholic fatty liver disease: from steatosis to cirrhosis. Hepatology 43 (2006) S99-S112
33. Yamagishi S., Okamoto T., Amano S., Inagaki Y., Koga K., Koga M., Choei H., Sasaki N., Kikuchi S., Takeuchi M., and Makita Z. Palmitate-induced apoptosis of microvascular endothelial cells and pericytes. Mol. Med. 8 (2002) 179-184
34. Maedler K., Oberholzer J., Bucher P., Spinas G.A., and Donath M.Y. Monounsaturated fatty acids prevent the deleterious effects of palmitate and high glucose on human pancreatic beta-cell turnover and function. Diabetes 52 (2003) 726-733
35. Miller T.A., LeBrasseur N.K., Cote G.M., Trucillo M.P., Pimentel D.R., Ido Y., Ruderman N.B., and Sawyer D.B. Oleate prevents palmitate-induced cytotoxic stress in cardiac myocytes. Biochem. Biophys. Res. Commun. 336 (2005) 309-315
36. Listenberger L.L., Ory D.S., and Schaffer J.E. Palmitate-induced apoptosis can occur through a ceramide-independent pathway. J. Biol. Chem. 276 (2001) 14890-14895
37. Ji J., Zhang L., Wang P., Mu Y.M., Zhu X.Y., Wu Y.Y., Yu H., Zhang B., Chen S.M., and Sun X.Z. Saturated free fatty acid, palmitic acid, induces apoptosis in fetal hepatocytes in culture. Exp. Toxicol. Pathol. 56 (2005) 336-369
38. Wei Y., Wang D., Topczewski F., and Pagliassotti M.J. Saturated fatty acids induce endoplasmic reticulum stress and apoptosis independently of ceramide in liver cells. Am. J. Physiol. Endocrinol. Metab. 291 (2006) E275-E281
39. Eitel K., Staiger H., Brendel M.D., Brandhorst D., Bretzel R.G., Haring H.U., and Kellerer M. Different role of saturated and unsaturated fatty acids in beta-cell apoptosis. Biochem. Biophys. Res. Commun. 299 (2002) 853-856
40. Kong J.Y., and Rabkin S.W. Palmitate-induced apoptosis in cardiomyocytes is mediated through alterations in mitochondria: prevention by cyclosporin A. Biochim. Biophys. Acta 1485 (2000) 45-55
41. Lu Z.H., Mu Y.M., Wang B.A., Li X.L., Lu J.M., Li J.Y., Pan C.Y., Yanase T., and Nawata H. Saturated free fatty acids, palmitic acid and stearic acid, induce apoptosis by stimulation of ceramide generation in rat testicular Leydig cell. Biochem. Biophys. Res. Commun. 303 (2003) 1002-1007