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Volumn 3, Issue 9, 2006, Pages 707-714

Lessons from Nature: microRNA-based shRNA libraries

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; CRE RECOMBINASE; DNA DIRECTED RNA POLYMERASE III; GREEN FLUORESCENT PROTEIN; LENTIVIRUS VECTOR; MESSENGER RNA; MICRORNA; OLIGONUCLEOTIDE; PROTEASOME; RNA POLYMERASE; SHORT HAIRPIN RNA;

EID: 33747754760     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth923     Document Type: Article
Times cited : (231)

References (39)
  • 1
    • 0034737298 scopus 로고    scopus 로고
    • RNAi: Double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals
    • Zamore, P.D., Tuschl, T., Sharp, P.A. & Bartel, D.P. RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell 101, 25-33 (2000).
    • (2000) Cell , vol.101 , pp. 25-33
    • Zamore, P.D.1    Tuschl, T.2    Sharp, P.A.3    Bartel, D.P.4
  • 2
    • 0033615491 scopus 로고    scopus 로고
    • A species of small antisense RNA in posttranscriptional gene silencing in plants
    • Hamilton, A.J.B. D.C. A species of small antisense RNA in posttranscriptional gene silencing in plants. Science 286, 950-952 (1999).
    • (1999) Science , vol.286 , pp. 950-952
    • Hamilton, A.J.B.D.C.1
  • 3
    • 0034673638 scopus 로고    scopus 로고
    • An RNA-directed nuclease mediates posttranscriptional gene silencing in Drosophila cells
    • Hammond, S.M., Bernstein, E., Beach, D. & Hannon, G.J. An RNA-directed nuclease mediates posttranscriptional gene silencing in Drosophila cells. Nature 404, 293-296 (2000).
    • (2000) Nature , vol.404 , pp. 293-296
    • Hammond, S.M.1    Bernstein, E.2    Beach, D.3    Hannon, G.J.4
  • 4
    • 0035905766 scopus 로고    scopus 로고
    • Role for a bidentate ribonuclease in the initiation step of RNA interference
    • Bernstein, E., Caudy, A.A., Hammond, S.M. & Hannon, G.J. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409, 363-366 (2001).
    • (2001) Nature , vol.409 , pp. 363-366
    • Bernstein, E.1    Caudy, A.A.2    Hammond, S.M.3    Hannon, G.J.4
  • 5
    • 0035839109 scopus 로고    scopus 로고
    • Argonaute2, a link between genetic and biochemical analyses of RNAi
    • Hammond, S.M., Boettcher, S., Caudy, A.A., Kobayashi, R. & Hannon, G.J. Argonaute2, a link between genetic and biochemical analyses of RNAi. Science 293, 1146-1150 (2001).
    • (2001) Science , vol.293 , pp. 1146-1150
    • Hammond, S.M.1    Boettcher, S.2    Caudy, A.A.3    Kobayashi, R.4    Hannon, G.J.5
  • 6
  • 7
    • 4444368187 scopus 로고    scopus 로고
    • Argonaute2 is the catalytic engine of mammalian RNAi
    • Liu, J. et al. Argonaute2 is the catalytic engine of mammalian RNAi. Science 305, 1437-1441 (2004).
    • (2004) Science , vol.305 , pp. 1437-1441
    • Liu, J.1
  • 8
    • 4444302947 scopus 로고    scopus 로고
    • Crystal structure of argonaute and its implications for RISC slicer activity
    • Song, J-J., Smith, S.K., Hannon, G.J. & Joshua-Tor, L. Crystal structure of argonaute and its implications for RISC slicer activity. Science 305, 1434-1437 (2004).
    • (2004) Science , vol.305 , pp. 1434-1437
    • Song, J.-J.1    Smith, S.K.2    Hannon, G.J.3    Joshua-Tor, L.4
  • 9
    • 10744225153 scopus 로고    scopus 로고
    • Asymmetry in the assembly of the RNAi enzyme complex
    • Schwarz, D.S. et al. Asymmetry in the assembly of the RNAi enzyme complex. Cell 115, 199-208 (2003).
    • (2003) Cell , vol.115 , pp. 199-208
    • Schwarz, D.S.1
  • 10
    • 0142165224 scopus 로고    scopus 로고
    • Functional siRNAs and miRNAs exhibit strand bias
    • Khvorova, A., Reynolds, A. & Jayasena, S.D. Functional siRNAs and miRNAs exhibit strand bias. Cell 115, 209-216 (2003).
    • (2003) Cell , vol.115 , pp. 209-216
    • Khvorova, A.1    Reynolds, A.2    Jayasena, S.D.3
  • 11
    • 0027751663 scopus 로고
    • The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarily to lin-14
    • Lee, R.C., Feinbaum, R.L. & Ambros, V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarily to lin-14. Cell 75, 843-854 (1993).
    • (1993) Cell , vol.75 , pp. 843-854
    • Lee, R.C.1    Feinbaum, R.L.2    Ambros, V.3
  • 12
    • 0025942586 scopus 로고
    • Negative regulatory sequences in the lin-14 3′-untranslated region are necessary to generate a temporal switch during Caenorhabditis elegans development
    • Wightman, B., Burglin, T.R., Gatto, J., Arasu, P. & Ruvkun, G. Negative regulatory sequences in the lin-14 3′-untranslated region are necessary to generate a temporal switch during Caenorhabditis elegans development. Genes Dev. 5, 1813-1824 (1993).
    • (1993) Genes Dev. , vol.5 , pp. 1813-1824
    • Wightman, B.1    Burglin, T.R.2    Gatto, J.3    Arasu, P.4    Ruvkun, G.5
  • 13
    • 0347444723 scopus 로고    scopus 로고
    • MicroRNAs: Genomics, biogenesis, mechanism, and function
    • Bartel, D.P. MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell 116, 281-297 (2004).
    • (2004) Cell , vol.116 , pp. 281-297
    • Bartel, D.P.1
  • 14
    • 3042767202 scopus 로고    scopus 로고
    • MicroRNAs: Small RNAs with a big role in gene regulation
    • He, L. & Hannon, G.J. MicroRNAs: Small RNAs with a big role in gene regulation. Nat. Rev. Genet. 5, 522-531 (2004).
    • (2004) Nat. Rev. Genet. , vol.5 , pp. 522-531
    • He, L.1    Hannon, G.J.2
  • 15
    • 8144225486 scopus 로고    scopus 로고
    • MicroRNA genes are transcribed by RNA polymerase II
    • Lee, Y. et al. MicroRNA genes are transcribed by RNA polymerase II. EMBO J. 23, 4051-4060 (2004).
    • (2004) EMBO J. , vol.23 , pp. 4051-4060
    • Lee, Y.1
  • 16
    • 9344235449 scopus 로고    scopus 로고
    • Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs
    • Cai, X., Hagedorn, C.H. & Cullen, B.R. Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs. RNA 10, 1957-1966 (2004).
    • (2004) RNA , vol.10 , pp. 1957-1966
    • Cai, X.1    Hagedorn, C.H.2    Cullen, B.R.3
  • 17
    • 0141843656 scopus 로고    scopus 로고
    • The nuclear RNase III Drosha initiates microRNA processing
    • Lee, Y. et al. The nuclear RNase III Drosha initiates microRNA processing. Nature 425, 415-419 (2003).
    • (2003) Nature , vol.425 , pp. 415-419
    • Lee, Y.1
  • 18
    • 9144224451 scopus 로고    scopus 로고
    • Processing of primary microRNAs by the Microprocessor complex
    • Denli, A.M., Tops, B.B., Plasterk, R.H., Ketting, R.F. & Hannon, G.J. Processing of primary microRNAs by the Microprocessor complex. Nature 432, 231-235 (2004).
    • (2004) Nature , vol.432 , pp. 231-235
    • Denli, A.M.1    Tops, B.B.2    Plasterk, R.H.3    Ketting, R.F.4    Hannon, G.J.5
  • 19
    • 10344248903 scopus 로고    scopus 로고
    • The human DiGeorge syndrome critical region gene 8 and its D. melanogaster homolog are required for miRNA biogenesis
    • Landthaler, M., Yalcin, A. & Tuschl, T. The human DiGeorge syndrome critical region gene 8 and its D. melanogaster homolog are required for miRNA biogenesis. Curr. Biol. 14, 2162-2167 (2004).
    • (2004) Curr. Biol. , vol.14 , pp. 2162-2167
    • Landthaler, M.1    Yalcin, A.2    Tuschl, T.3
  • 20
    • 10644234841 scopus 로고    scopus 로고
    • The Drosha-DGCR8 complex in primary microRNA processing
    • Han, J. et al. The Drosha-DGCR8 complex in primary microRNA processing. Genes Dev. 18, 3016-3027 (2004).
    • (2004) Genes Dev. , vol.18 , pp. 3016-3027
    • Han, J.1
  • 21
    • 9144225636 scopus 로고    scopus 로고
    • The Microprocessor complex mediates the genesis of microRNAs
    • Gregory, R.I. et al. The Microprocessor complex mediates the genesis of microRNAs. Nature 432, 235-240 (2004).
    • (2004) Nature , vol.432 , pp. 235-240
    • Gregory, R.I.1
  • 22
    • 33744520104 scopus 로고    scopus 로고
    • Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex
    • Han, J. et al. Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex. Cell 125, 887-901 (2006).
    • (2006) Cell , vol.125 , pp. 887-901
    • Han, J.1
  • 23
    • 0347361541 scopus 로고    scopus 로고
    • Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs
    • Yi, R., Qin, Y., Macara, I.G. & Cullen, B.R. Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs. Genes Dev. 17, 3011-3016 (2003).
    • (2003) Genes Dev. , vol.17 , pp. 3011-3016
    • Yi, R.1    Qin, Y.2    Macara, I.G.3    Cullen, B.R.4
  • 25
    • 13844280638 scopus 로고    scopus 로고
    • Synthetic shRNAs as potent RNAi triggers
    • Siolas, D. et al. Synthetic shRNAs as potent RNAi triggers. Nat. Biotechnol. 23, 227-231 (2005).
    • (2005) Nat. Biotechnol. , vol.23 , pp. 227-231
    • Siolas, D.1
  • 26
    • 0345490960 scopus 로고    scopus 로고
    • The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes
    • Song, J.J. et al. The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes. Nat. Struct. Biol. 10, 1026-1032 (2003).
    • (2003) Nat. Struct. Biol. , vol.10 , pp. 1026-1032
    • Song, J.J.1
  • 27
    • 0037089589 scopus 로고    scopus 로고
    • Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells
    • Paddison, P.J., Caudy, A.A., Bernstein, E., Hannon, G.J. & Conklin, D.S. Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev. 16, 948-958 (2002).
    • (2002) Genes Dev. , vol.16 , pp. 948-958
    • Paddison, P.J.1    Caudy, A.A.2    Bernstein, E.3    Hannon, G.J.4    Conklin, D.S.5
  • 28
    • 12144288006 scopus 로고    scopus 로고
    • A resource for large-scale RNA-interference-based screens in mammals
    • Paddison, P.J. et al. A resource for large-scale RNA-interference-based screens in mammals. Nature 428, 427-431 (2004).
    • (2004) Nature , vol.428 , pp. 427-431
    • Paddison, P.J.1
  • 29
    • 0036294527 scopus 로고    scopus 로고
    • Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells
    • Zeng, Y., Wagner, E.J. & Cullen, B.R. Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells. Mol. Cell 9, 1327-1333 (2002).
    • (2002) Mol. Cell , vol.9 , pp. 1327-1333
    • Zeng, Y.1    Wagner, E.J.2    Cullen, B.R.3
  • 30
    • 27644436787 scopus 로고    scopus 로고
    • Second-generation shRNA libraries covering the mouse and human genomes
    • Silva, J.M. et al. Second-generation shRNA libraries covering the mouse and human genomes. Nat. Genet. 37, 1281-1288 (2005).
    • (2005) Nat. Genet. , vol.37 , pp. 1281-1288
    • Silva, J.M.1
  • 31
    • 20444376939 scopus 로고    scopus 로고
    • A genetic screen for candidate tumor suppressors identifies REST
    • Westbrook, T.F. et al. A genetic screen for candidate tumor suppressors identifies REST. Cell 121, 837-848 (2005).
    • (2005) Cell , vol.121 , pp. 837-848
    • Westbrook, T.F.1
  • 32
    • 13844281898 scopus 로고    scopus 로고
    • Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy
    • Kim, D. et al. Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy. Nat. Biotechnol. 23, 222-226 (2005).
    • (2005) Nat. Biotechnol. , vol.23 , pp. 222-226
    • Kim, D.1
  • 33
    • 0346727524 scopus 로고    scopus 로고
    • MicroRNAs modulate hematopoietic lineage differentiation
    • Chen, C.Z., Li, L., Lodish, H.F. & Bartel, D.P. MicroRNAs modulate hematopoietic lineage differentiation. Science 303, 83-86 (2004).
    • (2004) Science , vol.303 , pp. 83-86
    • Chen, C.Z.1    Li, L.2    Lodish, H.F.3    Bartel, D.P.4
  • 34
    • 0037273432 scopus 로고    scopus 로고
    • Sequence requirements for micro RNA processing and function in human cells
    • Zeng, Y. & Cullen, B.R. Sequence requirements for micro RNA processing and function in human cells. RNA 9, 112-123 (2003).
    • (2003) RNA , vol.9 , pp. 112-123
    • Zeng, Y.1    Cullen, B.R.2
  • 35
    • 0026685057 scopus 로고
    • Structural elements of ornithine decarboxylase required for intracellular degradation and polyamine-dependent regulation
    • Ghoda, L., Sidney, D., Macrae, M. & Coffino, P. Structural elements of ornithine decarboxylase required for intracellular degradation and polyamine-dependent regulation. Mol. Cell. Biol. 12, 2178-2185 (1992).
    • (1992) Mol. Cell. Biol. , vol.12 , pp. 2178-2185
    • Ghoda, L.1    Sidney, D.2    Macrae, M.3    Coffino, P.4
  • 36
    • 24944560377 scopus 로고    scopus 로고
    • A lentiviral microRNA-based system for single copy Pol II regulated RNAi in mammalian cells
    • Stegmeier, F, Hu, G., Rickles, R.J., Hannon, G.J. & Elledge, S.J. A lentiviral microRNA-based system for single copy Pol II regulated RNAi in mammalian cells. Proc. Natl. Acad. Sci. USA 102, 13212-13217 (2005).
    • (2005) Proc. Natl. Acad. Sci. USA , vol.102 , pp. 13212-13217
    • Stegmeier, F.1    Hu, G.2    Rickles, R.J.3    Hannon, G.J.4    Elledge, S.J.5
  • 37
    • 27644582893 scopus 로고    scopus 로고
    • Probing tumor phenotypes using stable and regulated synthetic microRNA precursors
    • Dickins, R.A. et al. Probing tumor phenotypes using stable and regulated synthetic microRNA precursors. Nat. Genet. 37, 1289-1295 (2005).
    • (2005) Nat. Genet. , vol.37 , pp. 1289-1295
    • Dickins, R.A.1
  • 38
    • 15044348273 scopus 로고    scopus 로고
    • MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules
    • Li, M.Z. & Elledge, S.J. MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules. Nat. Genet. 37, 311-319 (2005).
    • (2005) Nat. Genet. , vol.37 , pp. 311-319
    • Li, M.Z.1    Elledge, S.J.2
  • 39
    • 33745253109 scopus 로고    scopus 로고
    • Identification and validation of oncogenes in liver cancer using an integrative oncogenomic approach
    • Zender, L. et al. Identification and validation of oncogenes in liver cancer using an integrative oncogenomic approach. Cell 125, 1253-1267 (2006).
    • (2006) Cell , vol.125 , pp. 1253-1267
    • Zender, L.1


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