In vivo quantitative studies of dynamic intracellular processes using fluorescence correlation spectroscopy

Biophysical Journal

Volumn 91, Issue 1, 2006, Pages 343-351

  Wang, Zifu ^a,b   Shah, Jagesh V ^d,f   Berns, Michael W ^b,c,e   Cleveland, Don W ^d  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c University of California at Irvine   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

^f HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANAPHASE PROMOTING COMPLEX; CELL CYCLE PROTEIN 20;

ANIMAL CELL; ARTICLE; CELL CYCLE; CONTROLLED STUDY; DIFFUSION COEFFICIENT; FLOW CYTOMETRY; FLUORESCENCE CORRELATION SPECTROSCOPY; HUMAN; HUMAN CELL; IN VIVO STUDY; INTERPHASE; INTRACELLULAR SPACE; MITOSIS; NONHUMAN; QUANTITATIVE STUDY; TELOPHASE;

EID: 33745764033     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1529/biophysj.105.077891     Document Type: Article

References (45)

1. Minshull, J., J. J. Blow, and T. Hunt. 1989. Translation of cyclin messenger-RNA is necessary for extracts of activated xenopus eggs to enter mitosis. Cell. 56:947-956.
2. Murray, A. W., and M. W. Kirschner. 1989. Cyclin synthesis drives the early embryonic-cell cycle. Nature. 339:275-280.
3. Page, A. M., and P. Hieter. 1999. The anaphase-promoting complex: new subunits and regulators. Annu. Rev. Biochem. 68:583-609.
4. Peters, J. M. 2002. The anaphase-promoting complex: proteolysis in mitosis and beyond. Mol. Cell. 9:931-943.
5. Harper, J. W., J. L. Burton, and M. J. Solomon. 2002. The anaphase-promoting complex: it's not just for mitosis any more. Genes Dev. 16:2179-2206.
6. Shah, J. V., and D. W. Cleveland. 2000. Waiting for anaphase: Mad2 and the spindle assembly checkpoint. Cell. 103:997-1000.
7. Glotzer, M., A. W. Murray, and M. W. Kirschner. 1991. Cyclin is degraded by the ubiquitin pathway. Nature. 349:132-138.
8. Fang, G. W., H. T. Yu, and M. W. Kirschner. 1998. Direct binding of CDC20 protein family members activates the anaphase-promoting complex in mitosis and G1. Mol. Cell. 2:163-171.
9. Tang, Z., H. Shu, D. Oncel, S. Chen, and H. Yu. 2004. Phosphorylation of Cdc20 by Bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint. Mol. Cell. 16:387-397.
10. Fang, G. W., H. T. Yu, and M. W. Kirschner. 1998. The checkpoint protein MAD2 and the mitotic regulator CDC20 form a ternary complex with the anaphase-promoting complex to control anaphase initiation. Genes Dev. 12:1871-1883.
11. Tang, Z. Y., R. Bharadwaj, B. Li, and H. T. Yu. 2001. Mad2-independent inhibition of APC(Cdc20) by the mitotic checkpoint protein BubR1. Dev. Cell. 1:227-237.
12. Sudakin, V., G. K. T. Chan, and T. J. Yen. 2001. Checkpoint inhibition of the APC/C in Hela Cells is mediated by a complex of BURB1, BUB3, CDC20, and MAD2. J. Cell Biol. 154:925-936.
13. Murray, A. W. 2004. Recycling the cell cycle: cyclins revisited. Cell. 116:221-234.
14. Chen, R. H., A. Shevchenko, M. Mann, and A. W. Murray. 1998. Spindle checkpoint protein Xmad1 recruits Xmad2 to unattached kinetochores. J. Cell Biol. 143:283-295.
15. Chan, G. K., and T. J. Yen. 2003. The mitotic checkpoint: a signaling pathway that allows a single unattached kinetochore to inhibit mitotic exit. In Progress in Cell Cycle Research. L. Meijer, A. Jezequel, and M. Roberge, editors. Springer, New York. 431-439.
16. Reimann, J. D. R., E. Freed, J. Y. Hsu, E. R. Kramer, J. M. Peters, and P. K. Jackson. 2001. Emi1 is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Cell. 105:645-655.
17. Hsu, J. Y., J. D. R. Reimann, C. S. Sorensen, J. Lukas, and P. K. Jackson. 2002. E2F-dependent accumulation of hEmi1 regulates S phase entry by inhibiting APC(Cdh1). Nat. Cell Biol. 4:358-366.
18. Yu, H. T. 2002. Regulation of APC-Cdc20 by the spindle checkpoint. Curr. Opin. Cell Biol. 14:706-714.
19. Elson, E. L., and D. Magde. 1974. Fluorescence correlation spectroscopy. 1. Conceptual basis and theory. Biopolymers. 13:1-27.
20. Magde, D., W. W. Webb, and E. Elson. 1972. Thermodynamic fluctuations in a reacting system - measurement by fluorescence correlation spectroscopy. Phys. Rev. Lett. 29:705-708.
21. Berland, K. M., P. T. C. So, and E. Gratton. 1995. 2-Photon fluorescence correlation spectroscopy - method and application to the intracellular environment. Biophys. J. 68:694-701.
22. Brock, R., M. A. Hink, and T. M. Jovin. 1998. Fluorescence correlation microscopy of cells in the presence of autofluorescence. Biophys. J. 75:2547-2557.
23. Schwille, P., U. Haupts, S. Maiti, and W. W. Webb. 1999. Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation. Biophys. J. 77:2251-2265.
24. Chen, Y., J. D. Muller, Q. Q. Ruan, and E. Gratton. 2002. Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy. Biophys. J. 82:133-144.
25. Kohler, R. H., P. Schwille, W. W. Webb, and M. R. Hanson. 2000. Active protein transport through plastid tubules: velocity quantified by fluorescence correlation spectroscopy. J. Cell Sci. 113:3921-3930.
26. Wang, Z., J. V. Shah, Z. Chen, C. H. Sun, and M. W. Berns. 2004. Fluorescence correlation spectroscopy investigation of a GFP mutant-enhanced cyan fluorescent protein and its tubulin fusion in living cells with two-photon excitation. J. Biomed. Opt. 9:395-403.
27. Larson, D. R., Y. M. Ma, V. M. Vogt, and W. W. Webb. 2003. Direct measurement of Gag-Gag interaction during retrovirus assembly with FRET and fluorescence correlation spectroscopy. J. Cell Biol. 162:1233-1244.
28. Shah, J. V., E. Botvinick, Z. Bonday, F. Furnari, M. Berns, and D. W. Cleveland. 2004. Dynamics of centromere and kinetochore proteins; implications for checkpoint signaling and silencing. Curr. Biol. 14:942-952.
29. Thompson, N. L. 1991. Fluorescence correlation spectroscopy. In Topics in Fluorescence Spectroscopy. J. R. Lakowicz, editor. Plenum, New York. 337-378.
30. Hess, S. T., S. H. Huang, A. A. Heikal, and W. W. Webb. 2002. Biological and chemical applications of fluorescence correlation spectroscopy: a review. Biochemistry. 41:697-705.
31. Chen, Y., J. D. Muller, K. M. Berland, and E. Gratton. 1999. Fluorescence fluctuation spectroscopy. Methods. 19:234-252.
32. Meseth, U., T. Wohland, R. Rigler, and H. Vogel. 1999. Resolution of fluorescence correlation measurements. Biophys. J. 76:1619-1631.
33. Kallio, M. J., V. A. Beardmore, J. Weinstein, and G. J. Gorbsky. 2002. Rapid microtubule-independent dynamics of Cdc20 at kinetochores and centrosomes in mammalian cells. J. Cell Biol. 158:841-847.
34. Howell, B. J., B. Moree, E. M. Farrar, S. Stewart, G. Fang, and E. D. Salmon. 2004. Spindle checkpoint protein dynamics at kinetochores in living cells. Curr. Biol. 14:953-964.
35. Dong, X. Z., K. H. Zavitz, B. J. Thomas, M. Lin, S. Campbell, and S. L. Zipursky. 1997. Control of G1 in the developing Drosophila eye: rca1 regulates Cyclin A. Genes Dev. 11:94-105.
36. Reimann, J. D. R., B. E. Gardner, F. Margottin-Goguet, and P. K. Jackson. 2001. Emi1 regulates the anaphase-promoting complex by a different mechanism than Mad2 proteins. Genes Dev. 15:3278-3285.
37. Pfleger, C. M., and M. W. Kirschner. 2000. The KEN box: an APC recognition signal distinct from the D box targeted by Cdh1. Genes Dev. 14:655-665.
38. Prinz, S., E. S. Hwang, R. Visintin, and A. Amon. 1998. The regulation of Cdc20 proteolysis reveals a role for the APC components Cdc23 and Cdc27 during S phase and early mitosis. Curr. Biol. 8:750-760.
39. Shirayama, M., W. Zachariae, R. Ciosk, and K. Nasmyth. 1998. The polo-like kinase Cdc5p and the WD-repeat protein Cdc2Op/fizzy are regulators and substrates of the anaphase promoting complex in Saccharomyces cerevisiae. EMBO J. 17:1336-1349.
40. Zhang, Y. K., and E. Lees. 2001. Identification of an overlapping binding domain on Cdc20 for Mad2 and anaphase-promoting complex: model for spindle checkpoint regulation. Mol. Cell. Biol. 21:5190-5199.
41. Zachariae, W., and K. Nasmyth. 1999. Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev. 13:2039-2058.
42. den Elzen, N., and J. Pines. 2001. Cyclin a is destroyed in prometaphase and can delay chromosome alignment and anaphase. J. Cell Biol. 153:121-135.
43. Geley, S., E. Kramer, C. Gieffers, J. Gannon, J. M. Peters, and T. Hunt. 2001. Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin a starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint. J. Cell Biol. 153:137-147.
44. Dawson, I. A., S. Roth, and S. Artavanistsakonas. 1995. The Drosophila cell cycle gene fizzy is required for normal degradation of cyclins A and B during mitosis and has homology to the CDC20 gene of Saccharomyces-cerevisiae. J. Cell Biol. 129:725-737.
45. Hames, R. S., S. L. Wattam, H. Yamano, R. Bacchieri, and A. M. Fry. 2001. APC/C-mediated destruction of the centrosomal kinase Nek2A occurs in early mitosis and depends upon a cyclin A-type D-box. EMBO J. 20:7117-7127.