RNA interference takes flight: a new RNAi screen reveals cell cycle regulators in Drosophila cells

Trends in Endocrinology and Metabolism

Volumn 17, Issue 5, 2006, Pages 173-174

  Lents, Nathan H ^a   Baldassare, Joseph J ^b  

^a NEW YORK UNIVERSITY MEDICAL CENTER   (United States)

^b SAINT LOUIS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

SMALL INTERFERING RNA;

ANIMAL CELL; CELL CYCLE; CELL CYCLE ARREST; CELL CYCLE G1 PHASE; CELL CYCLE G2 PHASE; CELL CYCLE PROGRESSION; CELL CYCLE REGULATION; CELL CYCLE S PHASE; CELL GROWTH; CELL PROLIFERATION; CYTOKINESIS; DROSOPHILA; FLUORESCENCE ACTIVATED CELL SORTING; GENETIC SCREENING; GENETIC TRANSFECTION; INSECT CELL; MITOSIS; NONHUMAN; PRIORITY JOURNAL; RNA INTERFERENCE; RNA VECTOR; SHORT SURVEY;

ANIMALS; CELL CYCLE; CELL CYCLE PROTEINS; CELLS, CULTURED; DROSOPHILA; GENE EXPRESSION PROFILING; GENETIC SCREENING; RNA INTERFERENCE; RNA, SMALL INTERFERING;

EID: 33745679153     PISSN: 10432760     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tem.2006.05.003     Document Type: Short Survey

References (18)

1. Hannon G.J. RNA interference. Nature 418 (2002) 244-251
2. Hammond S.M., et al. Post-transcriptional gene silencing by double-stranded RNA. Nat. Rev. Genet. 2 (2001) 110-119
3. Valencia-Sanchez M.A., et al. Control of translation and mRNA degradation by miRNAs and siRNAs. Genes Dev. 20 (2006) 515-524
4. Ketting R.F., et al. Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans. Genes Dev. 15 (2001) 2654-2659
5. Hammond S.M., et al. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature 404 (2000) 293-296
6. Elbashir S.M., et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411 (2001) 494-498
7. Paddison P.J., et al. Cloning of short hairpin RNAs for gene knockdown in mammalian cells. Nat Methods 1 (2004) 163-167
8. Bjorklund M., et al. Identification of pathways regulating cell size and cell-cycle progression by RNAi. Nature 439 (2006) 1009-1013
9. Kim Y.O., et al. A functional genomic screen for cardiogenic genes using RNA interference in developing Drosophila embryos. Proc. Natl. Acad. Sci. U. S. A. 101 (2004) 159-164
10. Echard A., et al. Terminal cytokinesis events uncovered after an RNAi screen. Curr. Biol. 14 (2004) 1685-1693
11. MacKeigan J.P., et al. Sensitized RNAi screen of human kinases and phosphatases identifies new regulators of apoptosis and chemoresistance. Nat. Cell Biol. 7 (2005) 591-600
12. Gwack, Y. et al. (2006) A genome-wide Drosophila RNAi screen identifies DYRK-family kinases as regulators of NFAT. Nature Epub ahead of print [doi:10.1038/nature04631]
13. Nybakken K., et al. A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. Nat. Genet. 37 (2005) 1323-1332
14. Westbrook T.F., et al. A genetic screen for candidate tumor suppressors identifies REST. Cell 121 (2005) 837-848
15. Kolfschoten I.G., et al. A genetic screen identifies PITX1 as a suppressor of RAS activity and tumorigenicity. Cell 121 (2005) 849-858
16. Berns K., et al. A large-scale RNAi screen in human cells identifies new components of the p53 pathway. Nature 428 (2004) 431-437
17. Brummelkamp T., et al. An shRNA barcode screen provides insight into cancer cell vulnerability to MDM2 inhibitors. Nat. Chem. Biol. 2 (2006) 202-206
18. Silva J.M., et al. Second-generation shRNA libraries covering the mouse and human genomes. Nat. Genet. 37 (2005) 1281-1288