메뉴 건너뛰기




Volumn 346, Issue 3, 2006, Pages 707-720

A novel siRNA validation system for functional screening and identification of effective RNAi probes in mammalian cells

Author keywords

AKT1; EGFP; Firefly luciferase; HBsAg; LMP1; MMP 7; p53; pDual; RNAi; siRNA

Indexed keywords

DNA FRAGMENT; ENHANCED GREEN FLUORESCENT PROTEIN; FIREFLY LUCIFERASE; GLUTATHIONE TRANSFERASE; HEPATITIS B SURFACE ANTIBODY; LATENT MEMBRANE PROTEIN 1; MATRILYSIN; PLASMID VECTOR; PROTEIN KINASE B; PROTEIN P53; PROTEIN SERINE THREONINE KINASE; SHORT HAIRPIN RNA; SMALL INTERFERING RNA; SYNTHETIC DNA; VIRUS VECTOR;

EID: 33745200043     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2006.05.164     Document Type: Article
Times cited : (30)

References (57)
  • 1
    • 0032545933 scopus 로고    scopus 로고
    • Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
    • Fire A., Xu S., Montgomery M.K., Kostas S.A., Driver S.E., and Mello C.C. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391 (1998) 806-811
    • (1998) Nature , vol.391 , pp. 806-811
    • Fire, A.1    Xu, S.2    Montgomery, M.K.3    Kostas, S.A.4    Driver, S.E.5    Mello, C.C.6
  • 2
    • 0037686295 scopus 로고    scopus 로고
    • Killing the messenger: short RNAs that silence gene expression
    • Dykxhoorn D.M., Novina C.D., and Sharp P.A. Killing the messenger: short RNAs that silence gene expression. Nat. Rev. Mol. Cell Biol. 4 (2003) 457-467
    • (2003) Nat. Rev. Mol. Cell Biol. , vol.4 , pp. 457-467
    • Dykxhoorn, D.M.1    Novina, C.D.2    Sharp, P.A.3
  • 3
    • 0042831010 scopus 로고    scopus 로고
    • Hairpin RNAs and retrotransposon LTRs effect RNAi and chromatin-based gene silencing
    • Schramke V., and Allshire R. Hairpin RNAs and retrotransposon LTRs effect RNAi and chromatin-based gene silencing. Science 301 (2003) 1069-1074
    • (2003) Science , vol.301 , pp. 1069-1074
    • Schramke, V.1    Allshire, R.2
  • 4
    • 13944266785 scopus 로고    scopus 로고
    • A potential role for RNA interference in controlling the activity of the human LINE-1 retrotransposon
    • Soifer H.S., Zaragoza A., Peyvan M., Behlke M.A., and Rossi J.J. A potential role for RNA interference in controlling the activity of the human LINE-1 retrotransposon. Nucleic Acids Res. 33 (2005) 846-856
    • (2005) Nucleic Acids Res. , vol.33 , pp. 846-856
    • Soifer, H.S.1    Zaragoza, A.2    Peyvan, M.3    Behlke, M.A.4    Rossi, J.J.5
  • 6
    • 0037072661 scopus 로고    scopus 로고
    • Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi
    • Volpe T.A., Kidner C., Hall I.M., Teng G., Grewal S.I., and Martienssen R.A. Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi. Science 297 (2002) 1833-1837
    • (2002) Science , vol.297 , pp. 1833-1837
    • Volpe, T.A.1    Kidner, C.2    Hall, I.M.3    Teng, G.4    Grewal, S.I.5    Martienssen, R.A.6
  • 7
    • 10644237968 scopus 로고    scopus 로고
    • RNAi-mediated pathways in the nucleus
    • Matzke M.A., and Birchler J.A. RNAi-mediated pathways in the nucleus. Nat. Rev. Genet. 6 (2005) 24-35
    • (2005) Nat. Rev. Genet. , vol.6 , pp. 24-35
    • Matzke, M.A.1    Birchler, J.A.2
  • 8
    • 0037448593 scopus 로고    scopus 로고
    • Functional genomics: RNA sets the standard
    • Tuschl T. Functional genomics: RNA sets the standard. Nature 421 (2003) 220-221
    • (2003) Nature , vol.421 , pp. 220-221
    • Tuschl, T.1
  • 11
    • 9144273425 scopus 로고    scopus 로고
    • RNA-interference-based functional genomics in mammalian cells: reverse genetics coming of age
    • Silva J., Chang K., Hannon G.J., and Rivas F.V. RNA-interference-based functional genomics in mammalian cells: reverse genetics coming of age. Oncogene 23 (2004) 8401-8409
    • (2004) Oncogene , vol.23 , pp. 8401-8409
    • Silva, J.1    Chang, K.2    Hannon, G.J.3    Rivas, F.V.4
  • 12
    • 0037107886 scopus 로고    scopus 로고
    • RNAi: gene-silencing in therapeutic intervention
    • Shuey D.J., McCallus D.E., and Giordano T. RNAi: gene-silencing in therapeutic intervention. Drug Discov. Today 7 (2002) 1040-1046
    • (2002) Drug Discov. Today , vol.7 , pp. 1040-1046
    • Shuey, D.J.1    McCallus, D.E.2    Giordano, T.3
  • 13
    • 4644350944 scopus 로고    scopus 로고
    • Unlocking the potential of the human genome with RNA interference
    • Hannon G.J., and Rossi J.J. Unlocking the potential of the human genome with RNA interference. Nature 431 (2004) 371-378
    • (2004) Nature , vol.431 , pp. 371-378
    • Hannon, G.J.1    Rossi, J.J.2
  • 14
    • 0348014774 scopus 로고    scopus 로고
    • Approaches for the sequence-specific knockdown of mRNA
    • Scherer L.J., and Rossi J.J. Approaches for the sequence-specific knockdown of mRNA. Nat. Biotechnol. 21 (2003) 1457-1465
    • (2003) Nat. Biotechnol. , vol.21 , pp. 1457-1465
    • Scherer, L.J.1    Rossi, J.J.2
  • 15
    • 0035942736 scopus 로고    scopus 로고
    • Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
    • Elbashir S.M., Harborth J., Lendeckel W., Yalcin A., Weber K., and Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411 (2001) 494-498
    • (2001) Nature , vol.411 , pp. 494-498
    • Elbashir, S.M.1    Harborth, J.2    Lendeckel, W.3    Yalcin, A.4    Weber, K.5    Tuschl, T.6
  • 16
    • 3242677639 scopus 로고    scopus 로고
    • siRNAs: applications in functional genomics and potential as therapeutics
    • Dorsett Y., and Tuschl T. siRNAs: applications in functional genomics and potential as therapeutics. Nat. Rev. Drug Discov. 3 (2004) 318-329
    • (2004) Nat. Rev. Drug Discov. , vol.3 , pp. 318-329
    • Dorsett, Y.1    Tuschl, T.2
  • 18
    • 0037134020 scopus 로고    scopus 로고
    • system for stable expression of short interfering RNAs in mammalian cells
    • Brummelkamp T.R., Bernards R., and Agami R.A. system for stable expression of short interfering RNAs in mammalian cells. Science 296 (2002) 550-553
    • (2002) Science , vol.296 , pp. 550-553
    • Brummelkamp, T.R.1    Bernards, R.2    Agami, R.A.3
  • 20
    • 0037089589 scopus 로고    scopus 로고
    • Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells
    • Paddison P.J., Caudy A.A., Bernstein E., Hannon G.J., and Conklin D.S. Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev. 16 (2002) 948-958
    • (2002) Genes Dev. , vol.16 , pp. 948-958
    • Paddison, P.J.1    Caudy, A.A.2    Bernstein, E.3    Hannon, G.J.4    Conklin, D.S.5
  • 21
    • 0036251639 scopus 로고    scopus 로고
    • Effective expression of small interfering RNA in human cells
    • Paul C.P., Good P.D., Winer I., and Engelke D.R. Effective expression of small interfering RNA in human cells. Nat. Biotechnol. 20 (2002) 505-508
    • (2002) Nat. Biotechnol. , vol.20 , pp. 505-508
    • Paul, C.P.1    Good, P.D.2    Winer, I.3    Engelke, D.R.4
  • 23
    • 0036245628 scopus 로고    scopus 로고
    • U6 promoter-driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells
    • Miyagishi M., and Taira K. U6 promoter-driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells. Nat. Biotechnol. 20 (2002) 497-500
    • (2002) Nat. Biotechnol. , vol.20 , pp. 497-500
    • Miyagishi, M.1    Taira, K.2
  • 26
    • 0037089135 scopus 로고    scopus 로고
    • Positional effects of short interfering RNAs targeting the human coagulation trigger tissue factor
    • Holen T., Amarzguioui M., Wiiger M.T., Babaie E., and Prydz H. Positional effects of short interfering RNAs targeting the human coagulation trigger tissue factor. Nucleic Acids Res. 30 (2002) 1757-1766
    • (2002) Nucleic Acids Res. , vol.30 , pp. 1757-1766
    • Holen, T.1    Amarzguioui, M.2    Wiiger, M.T.3    Babaie, E.4    Prydz, H.5
  • 27
  • 28
    • 0042161890 scopus 로고    scopus 로고
    • The activity of siRNA in mammalian cells is related to structural target accessibility: a comparison with antisense oligonucleotides
    • Kretschmer-Kazemi Far R., and Sczakiel G. The activity of siRNA in mammalian cells is related to structural target accessibility: a comparison with antisense oligonucleotides. Nucleic Acids Res. 31 (2003) 4417-4424
    • (2003) Nucleic Acids Res. , vol.31 , pp. 4417-4424
    • Kretschmer-Kazemi Far, R.1    Sczakiel, G.2
  • 29
    • 26444437334 scopus 로고    scopus 로고
    • siRNA target site secondary structure predictions using local stable substructures
    • Heale B.S.E., Soifer H.S., Bowers C., and Rossi J.J. siRNA target site secondary structure predictions using local stable substructures. Nucleic Acids Res. 33 (2005) e30
    • (2005) Nucleic Acids Res. , vol.33
    • Heale, B.S.E.1    Soifer, H.S.2    Bowers, C.3    Rossi, J.J.4
  • 30
    • 0142165224 scopus 로고    scopus 로고
    • Functional siRNAs and miRNAs exhibit strand bias
    • Khvorova A., Reynolds A., and Jayasena S.D. Functional siRNAs and miRNAs exhibit strand bias. Cell 115 (2003) 209-216
    • (2003) Cell , vol.115 , pp. 209-216
    • Khvorova, A.1    Reynolds, A.2    Jayasena, S.D.3
  • 32
    • 1942499451 scopus 로고    scopus 로고
    • Improving the efficiency of RNA interference in mammals
    • Mittal V. Improving the efficiency of RNA interference in mammals. Nat. Rev. Genet. 5 (2004) 355-365
    • (2004) Nat. Rev. Genet. , vol.5 , pp. 355-365
    • Mittal, V.1
  • 34
    • 0142092529 scopus 로고    scopus 로고
    • High-throughput selection of effective RNAi probes for gene silencing
    • Kumar R., Conklin D.S., and Mittal V. High-throughput selection of effective RNAi probes for gene silencing. Genome Res. 13 (2003) 2333-2340
    • (2003) Genome Res. , vol.13 , pp. 2333-2340
    • Kumar, R.1    Conklin, D.S.2    Mittal, V.3
  • 36
    • 17244367865 scopus 로고    scopus 로고
    • A rapid and sensitive assay for quantification of siRNA efficiency and specificity
    • Smart N., Scambler P.J., and Riley P.R. A rapid and sensitive assay for quantification of siRNA efficiency and specificity. Biol. Proced. Online 7 (2005) 1-7
    • (2005) Biol. Proced. Online , vol.7 , pp. 1-7
    • Smart, N.1    Scambler, P.J.2    Riley, P.R.3
  • 40
    • 1942509392 scopus 로고    scopus 로고
    • Matrilysin (matrix metalloproteinase-7): a new promising drug target in cancer and inflammation?
    • Wielockx B., Libert C., and Wilson C. Matrilysin (matrix metalloproteinase-7): a new promising drug target in cancer and inflammation?. Cytokine Growth Factor Rev. 15 (2004) 111-115
    • (2004) Cytokine Growth Factor Rev. , vol.15 , pp. 111-115
    • Wielockx, B.1    Libert, C.2    Wilson, C.3
  • 41
  • 42
    • 0032170456 scopus 로고    scopus 로고
    • Antisense to the Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP-1) suppresses LMP-1 and bcl-2 expression and promotes apoptosis in EBV-immortalized B cells
    • Kenney J.L., Guinness M.E., Curiel T., and Lacy J. Antisense to the Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP-1) suppresses LMP-1 and bcl-2 expression and promotes apoptosis in EBV-immortalized B cells. Blood 92 (1998) 1721-1727
    • (1998) Blood , vol.92 , pp. 1721-1727
    • Kenney, J.L.1    Guinness, M.E.2    Curiel, T.3    Lacy, J.4
  • 43
    • 0344630245 scopus 로고    scopus 로고
    • Epstein-Barr virus provides a survival factor to Burkitt's lymphomas
    • Kennedy G., Komano J., and Sugden B. Epstein-Barr virus provides a survival factor to Burkitt's lymphomas. Proc. Natl. Acad. Sci. USA 100 (2003) 14269-14274
    • (2003) Proc. Natl. Acad. Sci. USA , vol.100 , pp. 14269-14274
    • Kennedy, G.1    Komano, J.2    Sugden, B.3
  • 44
    • 0036632368 scopus 로고    scopus 로고
    • The phosphatidylinositol 3-Kinase AKT pathway in human cancer
    • Vivanco I., and Sawyers C.L. The phosphatidylinositol 3-Kinase AKT pathway in human cancer. Nat. Rev. Cancer 2 (2002) 489-501
    • (2002) Nat. Rev. Cancer , vol.2 , pp. 489-501
    • Vivanco, I.1    Sawyers, C.L.2
  • 46
    • 0037452812 scopus 로고    scopus 로고
    • A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA
    • Tiscornia G., Singer O., Ikawa M., and Verma I.M. A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA. Proc. Natl. Acad. Sci. USA 100 (2003) 1844-1848
    • (2003) Proc. Natl. Acad. Sci. USA , vol.100 , pp. 1844-1848
    • Tiscornia, G.1    Singer, O.2    Ikawa, M.3    Verma, I.M.4
  • 47
    • 0242331187 scopus 로고    scopus 로고
    • Inhibition of virus replication by RNA interference
    • Joost Haasnoot P.C., Cupac D., and Berkhout B. Inhibition of virus replication by RNA interference. J. Biomed. Sci. 10 (2003) 607-616
    • (2003) J. Biomed. Sci. , vol.10 , pp. 607-616
    • Joost Haasnoot, P.C.1    Cupac, D.2    Berkhout, B.3
  • 48
    • 0141941682 scopus 로고    scopus 로고
    • Human viruses under attack by small inhibitory RNA
    • Saksela K. Human viruses under attack by small inhibitory RNA. Trends Microbiol. 11 (2003) 345-347
    • (2003) Trends Microbiol. , vol.11 , pp. 345-347
    • Saksela, K.1
  • 49
    • 0037173613 scopus 로고    scopus 로고
    • Short interfering RNA confers intracellular antiviral immunity in human cells
    • Gitlin L., Karelsky S., and Andino R. Short interfering RNA confers intracellular antiviral immunity in human cells. Nature 418 (2002) 430-434
    • (2002) Nature , vol.418 , pp. 430-434
    • Gitlin, L.1    Karelsky, S.2    Andino, R.3
  • 50
    • 0037173610 scopus 로고    scopus 로고
    • Modulation of HIV-1 replication by RNA interference
    • Jacque J.M., Triques K., and Stevenson M. Modulation of HIV-1 replication by RNA interference. Nature 418 (2002) 435-438
    • (2002) Nature , vol.418 , pp. 435-438
    • Jacque, J.M.1    Triques, K.2    Stevenson, M.3
  • 51
    • 0037452625 scopus 로고    scopus 로고
    • Interference of hepatitis C virus RNA replication by short interfering RNAs
    • Kapadia S.B., Brideau-Andersen A., and Chisari F.V. Interference of hepatitis C virus RNA replication by short interfering RNAs. Proc. Natl. Acad. Sci. USA 100 (2003) 2014-2018
    • (2003) Proc. Natl. Acad. Sci. USA , vol.100 , pp. 2014-2018
    • Kapadia, S.B.1    Brideau-Andersen, A.2    Chisari, F.V.3
  • 52
    • 0037418322 scopus 로고    scopus 로고
    • RNA interference of influenza virus production by directly targeting mRNA for degradation and indirectly inhibiting all viral RNA transcription
    • Ge Q., McManus M.T., Nguyen T., Shen C.H., Sharp P.A., Eisen H.N., and Chen J. RNA interference of influenza virus production by directly targeting mRNA for degradation and indirectly inhibiting all viral RNA transcription. Proc. Natl. Acad. Sci. USA 100 (2003) 2718-2723
    • (2003) Proc. Natl. Acad. Sci. USA , vol.100 , pp. 2718-2723
    • Ge, Q.1    McManus, M.T.2    Nguyen, T.3    Shen, C.H.4    Sharp, P.A.5    Eisen, H.N.6    Chen, J.7
  • 53
    • 0036664838 scopus 로고    scopus 로고
    • Inhibition of viral gene expression and replication in mosquito cells by dsRNA-triggered RNA interference
    • Caplen N.J., Zheng Z., Falgout B., and Morgan R.A. Inhibition of viral gene expression and replication in mosquito cells by dsRNA-triggered RNA interference. Mol. Ther. 6 (2002) 243-251
    • (2002) Mol. Ther. , vol.6 , pp. 243-251
    • Caplen, N.J.1    Zheng, Z.2    Falgout, B.3    Morgan, R.A.4
  • 55
    • 0037382795 scopus 로고    scopus 로고
    • Inhibition of hepatitis B virus expression and replication by RNA interference
    • Shlomai A., and Shaul Y. Inhibition of hepatitis B virus expression and replication by RNA interference. Hepatology 37 (2003) 764-770
    • (2003) Hepatology , vol.37 , pp. 764-770
    • Shlomai, A.1    Shaul, Y.2
  • 57
    • 3142654211 scopus 로고    scopus 로고
    • The challenge of emerging and re-emerging infectious diseases
    • Morens D.M., Folkers G.K., and Fauci A.S. The challenge of emerging and re-emerging infectious diseases. Nature 430 (2004) 242-249
    • (2004) Nature , vol.430 , pp. 242-249
    • Morens, D.M.1    Folkers, G.K.2    Fauci, A.S.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.