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This manuscript is notable for its experimental design and data analysis, but most particularly for its conclusion: it is necessary to provide S. cerevisiae with regulatory instructions in addition to mechanisms for efficient xylose fermentation.
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Jin Y.S., Ni H., Laplaza J.M., and Jeffries T.W. Optimal growth and ethanol production from xylose by recombinant Saccharomyces cerevisiae require moderate d-xylulokinase activity. Appl Environ Microbiol 69 (2003) 495-503. This manuscript is notable for its experimental design and data analysis, but most particularly for its conclusion: it is necessary to provide S. cerevisiae with regulatory instructions in addition to mechanisms for efficient xylose fermentation.
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13C profiling to demonstrate the basic underlying differences in regulatory patterns in Pichia stipitis and Saccharomyces cerevisiae on xylose.
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Jin Y.S., Laplaza J.M., and Jeffries T.W. Saccharomyces cerevisiae engineered for xylose metabolism exhibits a respiratory response. Appl Environ Microbiol 70 (2004) 6816-6825. This paper is notable because it points out the need to engineer regulatory mechanisms in addition to the enzymatic machinery.
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Reports a novel and potentially useful way to navigate around the redox imbalance that is generated during growth on xylose.
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M for NADPH increases ethanol production from xylose in recombinant Saccharomyces cerevisiae. Biotechnol Bioeng (2005). Reports a novel and potentially useful way to navigate around the redox imbalance that is generated during growth on xylose.
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A comprehensive comparison of nine different S. cerevisiae strains that have been engineered and selected for xylose utilization.
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