Investigation of limiting metabolic steps in the utilization of xylose by recombinant Saccharomyces cerevisiae using metabolic engineering

Yeast

Volumn 22, Issue 5, 2005, Pages 359-368

  Karhumaa, Kaisa ^a   Hahn Hägerdal, Bärbel ^a   Gorwa Grauslund, Marie Francoise ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Ethanol; Pentose phosphate pathway; Screening strain; Xylose consumption; Xylose isomerase

Indexed keywords

ALCOHOL; ALDEHYDE REDUCTASE; FUNGAL ENZYME; PENTOSE PHOSPHATE; RIBOSEPHOSPHATE ISOMERASE; RIBULOSE 5 PHOSPHATE EPIMERASE; TRANSALDOLASE; TRANSKETOLASE; UNCLASSIFIED DRUG; XYLITOL; XYLOSE; XYLOSE ISOMERASE; XYLULOKINASE;

AEROBIC FERMENTATION; ALCOHOL PRODUCTION; ANAEROBIC FERMENTATION; ARTICLE; CARBOHYDRATE ANALYSIS; CARBOHYDRATE METABOLISM; ENZYME ACTIVITY; FUNGAL STRAIN; FUNGUS GROWTH; GENE DELETION; GENE FUNCTION; GENE OVEREXPRESSION; GENETIC CODE; NONHUMAN; PENTOSE PHOSPHATE CYCLE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; SPECIES CULTIVATION; STRAIN DIFFERENCE; THERMUS THERMOPHILUS;

ALDEHYDE REDUCTASE; ALDOSE-KETOSE ISOMERASES; D-XYLULOSE REDUCTASE; DNA, FUNGAL; FERMENTATION; GENETIC ENGINEERING; INDUSTRIAL MICROBIOLOGY; PENTOSE PHOSPHATE PATHWAY; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; SUGAR ALCOHOL DEHYDROGENASES; XYLOSE; XYLULOSE;

SACCHAROMYCES CEREVISIAE; THERMUS THERMOPHILUS;

EID: 17644373035     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.1216     Document Type: Article

References (35)

1. Becker J, Boles E. 2003. A modified Saccharomyces cerevisiae strain that consumes L-arabinose and produces ethanol. Appl Environ Microbiol 69: 4144-4150.
2. Bergmeyer H. 1974. Methods in Enzymatic Analysis, 2nd edn. New York: Academic Press.
3. Dagert M, Ehrlich SD. 1979. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene 6: 23-28.
4. Eliasson A, Christensson C, Wahlbom CF, Hahn-Hägerdal B. 2000. Anaerobic xylose fermentation by recombinant Saccharomyces cerevisiae carrying XYL1, XYL2, and XKS1 in mineral medium chemostat cultures. Appl Environ Microbiol 66: 3381-3386.
5. Entian KD, Kötter P. 1998. Yeast mutant and plasmid collections. In Yeast Gene Analysis, Brown JPA, Tuite MF (eds). Academic Press: New York; 431-449.
6. Gietz RD, Schiestl RH, Willems AR, Woods RA. 1995. Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure. Yeast 11: 355-360.
7. Gietz RD, Sugino A. 1988. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534.
8. Hahn-Hägerdal B, Wahlbom CF, Gardonyi M, et al. 2001. Metabolic engineering of Saccharomyces cerevisiae for xylose utilization. Adv Biochem Eng Biotechnol 73: 53-84.
9. Hauf J, Zimmermann FK, Muller S. 2000. Simultaneous genomic overexpression of seven glycolytic enzymes in the yeast Saccharomyces cerevisiae. Enzyme Microb Technol 26: 688-698.
10. Ho NW, Chen Z, Brainard AP. 1998. Genetically engineered Saccharomyces yeast capable of effective cofermentation of glucose and xylose. Appl Environ Microbiol 64: 1852-1859.
11. Johansson B, Christensson C, Hobley T, Hahn-Hägerdal B. 2001. Xylulokinase overexpression in two strains of Saccharomyces cerevisiae also expressing xylose reductase and xylitol dehydrogenase and its effect on fermentation of xylose and lignocellulosic hydrolysate. Appl Environ Microbiol 67: 4249-4255.
12. Johansson B, Hahn-Hägerdal B. 2002a. The non-oxidative pentose phosphate pathway controls the fermentation rate of xylulose but not of xylose in Saccharomyces cerevisiae TMB3001. FEMS Yeast Res 2: 277-282.
13. Johansson B, Hahn-Hägerdal B. 2002b. Overproduction of pentose phosphate pathway enzymes using a new CRE-loxP expression vector for repeated genomic integration in Saccharomyces cerevisiae. Yeast 19: 225-231.
14. Kuhn A, van Zyl C, van Tonder A, Prior BA. 1995. Purification and partial characterization of an aldo-keto reductase from Saccharomyces cerevisiae. Appl Environ Microbiol 61: 1580-1585.
15. Kuyper M, Harhangi HR, Stave AK, et al. 2003. High-level functional expression of a fungal xylose isomerase: the key to efficient ethanolic fermentation of xylose by Saccharomyces cerevisiae? FEMS Yeast Res 4: 69-78.
16. Kuyper M, Winkler AA, Van Dijken JP, Pronk JT. 2004. Minimal metabolic engineering of Saccharomyces cerevisiae for efficient anaerobic xylose fermentation: a proof of principle. FEMS Yeast Res 4: 655-664.
17. Lönn A, Träff-Bjerre KL, Cordero Otero RR, van Zyl WH, Hahn-Hägerdal B. 2003. Xylose isomerase activity influences xylose fermentation with recombinant Saccharomyces cerevisiae strains expressing mutated xylA from Thermus thermophilus. Enzyme Microb Technol 32: 567-573.
18. Miosga T, Zimmermann FK. 1996. Cloning and characterization of the first two genes of the non-oxidative part of the Saccharomyces cerevisiae pentose-phosphate pathway. Curr Genet 30: 404-409.
19. Olsson L, Linden T, Hahn-Hägerdal B. 1994. A rapid chromatographic method for the production of preparative amounts of xylulose. Enzyme Microb Technol 16: 388-394.
20. Richard P, Toivari MH, Penttilä M. 1999. Evidence that the gene YLR070c of Saccharomyces cerevisiae encodes a xylitol dehydrogenase. FEBS Lett 457: 135-138.
21. +-xylitol-dehydrogenase from the yeast Pichia stipitis. J Ferment Bioeng 67: 20-24.
22. Rosenberg SM. 2001. Evolving responsively: adaptive mutation. Nature Rev Genet 2: 504-515.
23. Sambrook J, Fritch E, Maniatis T. 1989. Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY.
24. Shamanna DK, Sanderson KE. 1979. Uptake and catabolism of D-xylose in Salmonella typhimurium LT2. J Bacteriol 139 64-70.
25. Smiley KL, Bolen PL. 1982. Demonstration of D-xylose reductase and D-xylitol dehydrogenase in Pachysolen tannophilus. Biotech Lett 4: 607-610.
26. Toivari MH, Aristidou A, Ruohonen L, Penttilä M. 2001. Conversion of xylose to ethanol by recombinant Saccharomyces cerevisiae: importance of xylulokinase (XKS1) and oxygen availability. Metab Eng 3: 236-249.
27. Träff KL, Jönsson LJ, Hahn-Hägerdal B. 2002. Putative xylose and arabinose reductases in Saccharomyces cerevisiae. Yeast 19: 1233-1241.
28. Träff KL, Otero Cordero RR, van Zyl WH, Hahn-Hägerdal B. 2001. Deletion of the GRE3 aldose reductase gene and its influence on xylose metabolism in recombinant strains of Saccharomyces cerevisiae expressing the xylA and XKS1 genes. Appl Environ Microbiol 67: 5668-5674.
29. Träff-Bjerre KL, Jeppsson M, Hahn-Hägerdal B, Gorwa-Grauslund MF. 2004. Endogenous NADPH-dependent aldose reductase activity influences product formation during xylose consumption in recombinant Saccharomyces cerevisiae. Yeast 21: 141-150.
30. Verduyn C, Postma E, Scheffers WA, van Dijken JP. 1992. Effect of benzoic acid on metabolic fluxes in yeasts: a continuous-culture study on the regulation of respiration and alcoholic fermentation. Yeast 8: 501-517.
31. von Sivers M, Zacchi G. 1996. Ethanol from lignocellulosics: a review of the economy. Biores Technol 56: 131-140.
32. Walfridsson M, Anderlund M, Bao X, Hahn-Hägerdal B. 1997. Expression of different levels of enzymes from the Pichia stipitis XYL1and XYL2 genes in Saccharomyces cerevisiae and its effects on product formation during xylose utilization. Appl Microbiol Biotechnol 48: 218-224.
33. Walfridsson M, Bao X, Anderlund M, et al. 1996. Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase. Appl Environ Microbiol 62: 4648-4651.
34. Walfridsson M, Hallborn J, Penttilä M, Keränen S, Hahn-Hägerdal B. 1995. Xylose-metabolizing Saccharomyces cerevisiae strains overexpressing the TKL1 and TAL1 genes encoding the pentose phosphate pathway enzymes transketolase and transaldolase. Appl Environ Microbiol 61: 4184-4190.
35. Yamanaka K. 1969. Inhibition of D-xylose isomerase by pentitols and D-lyxose. Arch Biochem Biophys 131: 502-506.