The extraordinary specificity of xanthine phosphoribosyltransferase from Bacillus subtilis elucidated by reaction kinetics, ligand binding, and crystallography

Biochemistry

Volumn 45, Issue 21, 2006, Pages 6615-6627

  Arent, Susan ^a,d   Kadziola, Anders ^a   Larsen, Sine ^a,b   Neuhard, Jan ^c   Jensen, Kaj Frank ^b  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b EUROPEAN SYNCHROTRON RADIATION FACILITY   (France)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

^d CARLSBERG LABORATORY   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BACTERIA; CARBOXYLATION; CATALYSIS; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; HYDROGEN BONDS; LIGAMENTS; OLIGOMERS; REACTION KINETICS; STABILIZATION;

BACILLUS SUBTILIS; CATALYTIC CARBOXYLATE GROUP; ELECTROSTATIC POTENTIAL; XANTHINE PHOSPHORIBOSYLTRANSFERASE (XPRTASE);

ENZYMES;

ADENINE; BACTERIAL ENZYME; CARBON 14; GUANINE; GUANOSINE PHOSPHATE; HYPOXANTHINE; INOSINE PHOSPHATE; PHOSPHORIBOSYLTRANSFERASE; PURINE; UNCLASSIFIED DRUG; XANTHINE; XANTHINE PHOSPHORIBOSYLTRANSFERASE; XANTHOSINE;

ARTICLE; BACILLUS SUBTILIS; CHEMICAL REACTION KINETICS; CRYSTAL STRUCTURE; CRYSTALLIZATION; ENZYME KINETICS; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE EXPRESSION; ISOTOPE LABELING; LIGAND BINDING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN FOLDING; STRUCTURE ANALYSIS; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; BACILLUS SUBTILIS; BASE SEQUENCE; CRYSTALLOGRAPHY; DNA PRIMERS; HYDROGEN BONDING; HYDROGEN-ION CONCENTRATION; KINETICS; LIGANDS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PENTOSYLTRANSFERASES; SEQUENCE HOMOLOGY, AMINO ACID;

BACILLUS SUBTILIS; POSIBACTERIA;

EID: 33744760238     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi060287y     Document Type: Article

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