Xanthine metabolism in Bacillus subtilis: Characterization of the xpt- pbuX operon and evidence for purine- and nitrogen-controlled expression of genes involved in xanthine salvage and catabolism

Journal of Bacteriology

Volumn 179, Issue 8, 1997, Pages 2540-2550

  Christiansen, Lynge C ^a   Schou, Simon ^b,c   Nygaard, Per ^a   Saxild, Hans H ^a,b  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c NOVO NORDISK A S   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

PERMEASE; PHOSPHORIBOSYLTRANSFERASE; PURINE; XANTHINE;

ARTICLE; BACILLUS SUBTILIS; GENE EXPRESSION REGULATION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PRIORITY JOURNAL; PURINE METABOLISM;

EID: 0030894628     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.8.2540-2550.1997     Document Type: Article

References (44)

1. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Andersen, P. S., D. Frees, R. Fast, and B. Mygind. 1995. Uracil uptake in Escherichia coli K-12: isolation of uraA mutants and cloning of the gene. J. Bacteriol. 177:2008-2013.
3. Atkinson, M. R., and S. H. Fisher. 1991. Identification of genes and gene products whose expression is activated during nitrogen-limited growth in Bacillus subtilis. J. Bacteriol. 173:23-27.
4. Beaman, T. C., A. D. Hitchins, K. Ochi, N. Vasantha, T. Endo, and E. Freese. 1983. Specificity and control of uptake of purines and other compounds in Bacillus subtilis. J. Bacteriol. 156:1107-1117.
5. Birnboim, H. C., and J. Doly. 1979. A rapid alkaline extraction procedure for screening rccombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
6. Brown, K., L., and K. T. Hughes. 1995. The role of anti-sigma factors in gene regulation. Mol. Microbiol. 16:397-404.
7. Burton, K. 1983. Transport of nucleic acid bases into Escherichia coli. J. Gen. Microbiol. 129:3505-3513.
8. Caramori, T., S. Calogero, A. M. Albertini, and A. Galizzi. 1993. Functional analysis of the outB gene of Bacillus subtilis. J. Gen. Microbiol. 139:31-37.
9. Danielsen, S., M. kilstrup, K. Barilla, B. Jochimsen, and J. Neuhard. 1992. Characterization of the Escherichia coli codBA operon encoding cytosine permease and cytosine deaminase. Mol. Microbiol. 6:1335-1344.
10. Diallinas, G., L. Gorfinkiel, H. N. Arst, Jr., G. Cecchetto, and C. Scazzocchio. 1995. Genetic and molecular characterization of a gene encoding a wide specificity purine permease of Aspergillus nidulans reveals a novel family of transporters conserved in procaryotes and eucaryotes. J. Biol. Chem. 270:8610-8622.
11. Ebbole, D. J., and H. Zalkin. 1987. Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide biosynthesis. J. Biol. Chem. 262:8274-8287.
12. Endo, T., B. Uratani, and E. Freese. 1983. Purine salvage pathways of Bacillus stthtilis and effect of guaninc on growth of GMP reductase mutants. J. Bacteriol. 155:169-179.
13. Errington, J. 1986. A general method for fusion of the Escherichia coli lacZ gene to chromosomal genes in Bacillus subtilis. J. Gen. Microbiol. 132:2953-2966.
14. Fisher, S. H. 1993. Utilization of amino acids and other nitrogen-containing compounds, p. 221-228. In A. L. Sonenshein. J. A. Hoch, and R. Losick (ed.). Bacillus subtilis and other gram-positive bacteria: biochemistry, physiology, and molecular genetics. American Society for Microbiology, Washington, D.C.
15. Glaser, P., A. Danchin, F. Kunst, P. Zuber, and M. M. Nakano. 1995. Identification and isolation of a gene required for nitrate assimilation and anaerobic growth of Bacillus subtilis. J. Bacteriol. 177:1112-1115.
16. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J Mol. Biol. 166:557-580.
17. Houlnerg, U., and K. F. Jensen. 1983. Role of hypoxanthine and guanine in regulation of Salmonella typhimurium pur gene expression. J. Bacteriol. 153:837-845.
18. Jensen, K. F., F. Bonekamp, and P. Poulsen. 1986. Attenuation at nucleotide biosynthetic genes and amino acid biosynthetic operons of Escherichia coli. Trends Biochem. Sci. 11:362-365.
19. Kelley, W. N., F. M. Rosenbloom, J. F. Henderson, and J. E. Seegmiller. 1967. Xanthine phosphoribosyltransferase in man: relationship to hypoxanthine-guanine phosphoribosyltransferase. Biochem. Biophys. Res. Commun. 28:340-345.
20. Krenitsky, T. A., S. M. Neil, and R. L. Miller. 1970. Guanine and xanthine phosphoribosyltransfer activities of Lactobacillus casei and Escherichia coli. J. Biol. Chem. 245:2605-2611.
21. Miller, J. H. 1972. Assay of β-gatectosidase, p. 352-355. In J. H. Miller (ed.), Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
22. Miller, R. L., D. L. Adamczyk, J. A. Fyfe, and G. B. Elion. 1974. Xanthine phosphoribosyltransferase from Streptococcus faecalis. Arch. Biochem. Biophys. 165:349-358.
23. Monod, J., J. G. Cohen-Bazire, and M. Cohn. 1951. Sur la biosynthese de la beta-galactosidase (factase) chez Escherichia coli. La specificite de l'induction. Biochim. Biophys. Acta 7:585-599.
24. Mulligan, R. C., and P. Berg. 1981. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Proc. Natl. Acad. Sci. USA 78:2072-2076.
25. Munch-Petersen, A., and B. Mygind. 1983. Transport of nucleic acid precursors, p. 259-305. In A. Munch-Petersen (ed.), Metabolism of nucleotides, nucleosides and nucleobases in microorganisms. Academic Press Ltd., London, United Kingdom.
26. Neuhard, J., and P. Nygaard. 1987. Purines and pyrimidines, p. 445-473. In F. C Neidhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology. Washington, D.C.
27. Nilsson, B., M. Uhlén, S. Josephson, S. Gatenbeck, and L. Phillipson. 1983. An improved positive selection plasmid vector constructed by oligonucleotide mediated mutagenesis. Nucleic Acids Res. 11:8019-8030.
28. Nygaard, P. 1983. Utilization of preformed purine bases and nucleosides. p. 27-93. In A. Munch-Petersen (ed.), Metabolism of nucleotides. nucleosides and nucleobases in microorganisms. Academic Press Ltd., London, United Kingdom.
29. Nygaard, P., P. Duckert, and H. H. Saxild. 1996. Role of adenine deaminase in purine salvage and nitrogen metabolism and characterization of the title gene in Bacillus subtilis. J. Bacteriol. 178:846-853.
30. Rouf, M. A., and R. F. Lomprey. 1968. Degradation of uric acid by certain aerobic bacteria. J. Bacteriol. 96:617-622.
31. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
32. Saxild, H. H., L. N. Andersen, and K. Hammer. 1996. dra-nupC-pdp operon of Bacillus subtilis: nucleotide sequence, induction by deoxyribonucleosides, and transcriptional regulation by the deoR-encoded DeoR repressor protein. J. Bacteriol. 178:424434.
33. Saxild, H. H., J. H. Jacobsen, and P. Nygaard. 1995. Functional analysis of the Bacillus subtilis purT gene encoding formate-dependent glycinamide ribonucleotide transformylase. Microbiology 141:2211-2218.
34. Saxild, H. H., and P. Nygaard. 1987. Genetic and physiological characterization of Bacillus subtilis mutants resistant to purine analogs. J. Bacteriol. 169:2977-2983.
35. Saxild, H. H., and P. Nygaard. Unpublished results.
36. Shimotsu, H., M. I. Kuroda, C. Yanofsky, and D. J. Henner. 1986. Novel form of transcription attenuation regulates expression of the Bacillus subtilis tryptophan operon. J. Bacteriol. 166:461-471.
37. Tinoco, I., Jr., P. N. Borer, B. Dengler, and M. D. Levine. 1973. Improved estimation of secondary structure in rihonucleic acids. Nature 246:40-41.
38. Tuerk, C., P. Gauss, C. Thermes, D. R. Groebe, M. Gayle, N. Guild, G. Stormo, Y. D'Aubenton-Carafa, O. C. Uhlenbeck, I. Tinoco, Jr., E. N. Brody, and L. Gold. 1988. CUUCGG hairpins: extraordinarily stable RNA secondary structures associated with various biochemical processes. Proc. Natl. Acad. Sci. USA 85:1364-1368.
39. Turner, R. J., Y. Lu, and R. L. Switzer. 1994. Regulation of the Bacillus sitbtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism. J. Bacteriol. 176:3708-3722.
40. Vogels, G. D., and C. van der Drift. 1976. Degradation of purines and pyrimidines by microorganisms. Bacteriol. Rev. 40:403-469.
41. von Heijne, G. 1994. Membrane proteins: from sequence to structure. Annu Rev. Biophys. Biomol. Struct. 23:167-192.
42. Watanabe, Y., T. Ohe, and Y. Tsujisaka. 1976. Changes in the metabolic pathways of hypoxanthine in Streptomyces. J. Gen. Appl. Microbiol. 22:13-23.
43. Weng, M., P. L. Nagy, and H. Zalkin. 1995. Identification of the Bacillus subtilis pur operon repressor. Proc. Natl. Acad. Sci. USA 92:7455-7459.
44. Zalkin, H., and D. J. Ebbole. 1988. Organization and regulation of genes encoding biosynthetic enzymes in Bacillus subtilis. J. Biol. Chem. 263:1595-1598.