Catalytic properties of dihydroorotate dehydrogenase from Saccharomyces cerevisiae: Studies on pH, alternate substrates, and inhibitors

Archives of Biochemistry and Biophysics

Volumn 378, Issue 1, 2000, Pages 84-92

  Jordan, Douglas B ^a   Bisaha, John J ^a   Picollelli, Michael A ^a  

^a DUPONT COMPANY   (United States)

Author keywords

Deuterium; Dihydroorotate dehydrogenase; Flavoprotein dehydrogenase; Kinetic isotope effects; Saccharomyces cerevisiae; Substrate specificity

Indexed keywords

DEUTERIUM; DIHYDROOROTATE DEHYDROGENASE; ENZYME INHIBITOR; FLAVOPROTEIN;

ARTICLE; BINDING AFFINITY; CATALYSIS; ENZYME ACTIVE SITE; ENZYME SUBSTRATE; GENE TARGETING; HYDROGENATION; LACTOCOCCUS LACTIS; MEMBRANE BINDING; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SOLUBILITY;

EID: 0034213171     PISSN: 00039861     EISSN: None     Source Type: Journal    
DOI: 10.1006/abbi.2000.1823     Document Type: Article

References (24)

1. Jones M. E. Annu. Rev. Biochem. 49:1980;253-279.
2. Howie C., Suckling C. J., Wood H. C. S. J. Chem. Soc., Perkin Trans. 1:1990;3129-3135.
3. Chen S. F., Perrella F. W., Behrens D. L., Papp L. M. Cancer Res. 52:1992;3521-3527.
4. Knecht W., Loffler M. Biochem. Pharmacol. 56:1998;1259-1264.
5. Batt D. B. Exp. Opin. Ther. Patents. 9:1999;41-54.
6. Nielsen F. S., Andersen P. S., Jensen K. F. J. Biol. Chem. 271:1996;29359-29365.
7. Nielsen F. S., Rowland P., Larsen S., Jensen K. F. Protein Sci. 5:1996;852-856.
8. Rowland P., Nielsen F., Jensen K. F., Larsen S. Structure. 5:1997;239-252.
9. Rowland P., Nielsen F. S., Jensen K. F., Larsen S. Acta Crystallogr. D53:1997;802-804.
10. Bjornberg O., Rowland P., Larsen S., Jensen K. F. Biochemistry. 36:1997;16197-16205.
11. Rowland P., Bjornberg O., Nielsen F. S., Jensen K. F., Larsen S. Protein Sci. 7:1998;1269-1279.
12. Bjornberg O., Gruner A.-C., Roepstorff P., Jensen K. F. Biochemistry. 38:1999;2899-2908.
13. Nagy M., Lacroute F., Thomas D. Proc. Natl. Acad. Sci. USA. 89:1992;8966-8970.
14. Pascal R. A. Jr., Walsh C. T. Biochemistry. 23:1984;2745-2752.
15. Blitz H., Giesler E. Chem. Ber. 46:1913;3410-3425.
16. Faeder E. J., Siegel C. M. Anal. Biochem. 53:1973;332-336.
17. Martin R. G., Ames B. N. J. Biol. Chem. 236:1961;1372-1379.
18. Roy A. Gene. 118:1992;149-150.
19. Bradford M. M. Anal. Biochem. 72:1976;248-254.
20. Guex N., Peitsch M. C. Electrophoresis. 18:1997;2714-2723.
21. Segel, I. H. 1975, Enzyme Kinetics, Wiley, New York.
22. Thompson J. E., Jordan D. B. Anal. Biochem. 256:1998;7-13.
23. Jordan D. B., Bisaha J. J., Picollelli M. A. Ghisla S., Kroneck P., Macheroux P., Sund H. Flavins and Flavoproteins 1999. 1999;611-614 Agency for Scientific Publ. Berlin.
24. Cleland W. W. Methods Enzymol. 87:1982;390-405.